scholarly journals Caffeine Consumption Influences Lidocaine Action via Pain-Related Voltage-Gated Sodium Channels: An In Vivo Animal Study

2022 ◽  
Vol 2022 ◽  
pp. 1-8
Author(s):  
Reham Alfaraj ◽  
Zainab Alabdulsalam ◽  
Zahrah Alfaraj ◽  
Hawraa Alsunni ◽  
Hussain Alhawaj ◽  
...  
Keyword(s):  
Sodium Channels ◽  
Dorsal Root ◽  
Caffeine Intake ◽  
Control Group ◽  
Intraplantar Injection ◽  
Caffeine Consumption ◽  
Voltage Gated ◽  
Thermal Pain ◽  
Voltage Gated Sodium Channels ◽  
Pressure Sensation

Several factors might influence the duration and efficiency of local anesthesia. This study investigates the effect of habitual caffeine intake on lidocaine action and explores the potential involvement of voltage-gated sodium channels in the interaction effect. Wistar rats were divided into four groups: (i) control (Ctrl), (ii) lidocaine intraplantar injection (LIDO), (iii) habitual caffeine intake (CAF), and (iv) lidocaine intraplantar injection and habitual caffeine intake (LIDO + CAF). Behavioral assessments, consisting of a paw pressure test for mechanical pressure sensation and a paw withdrawal latency test for thermal pain sensation, were performed at 0, 30, 60, and 90 minutes following lidocaine injection and after 10, 11, and 12 weeks of CAF intake. Pressure sensation was significantly reduced in the LIDO + CAF group compared with the control group. Moreover, the LIDO + CAF group exhibited reduced sensation compared to LIDO alone group. The LIDO + CAF combination exerted a synergistic effect at 30 and 60 minutes compared with the control. This synergistic effect was noted at 60 minutes (11 weeks of CAF intake) and at 30 minutes (12 weeks of CAF intake) compared with LIDO alone. Augmented thermal pain-relieving effects were observed in the LIDO + CAF group at all weeks compared to the control group and at 10 weeks compared to LIDO alone group. The molecular analysis of dorsal root ganglia suggested that CAF upregulated the mRNA expression of the Nav1.3, Nav1.7, and Nav1.8 sodium channel subtypes. Chronic caffeine consumption potentiates the local anesthetic action of lidocaine in an experimental animal model through mechanisms that involve the upregulation of voltage-gated sodium channels in the dorsal root ganglia.

scholarly journals Are Voltage-Gated Sodium Channels on the Dorsal Root Ganglion Involved in the Development of Neuropathic Pain?

2011 ◽  
Vol 7 ◽  
pp. 1744-8069-7-16 ◽  
Author(s):  
Wei Wang ◽  
Jianguo Gu ◽  
Yun-Qing Li ◽  
Yuan-Xiang Tao
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Sodium Channels ◽  
Dorsal Root ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels

Interleukin-10 down-regulates voltage gated sodium channels in rat dorsal root ganglion neurons

2013 ◽  
Vol 247 ◽  
pp. 466-475 ◽  
Author(s):  
Kai-Feng Shen ◽  
He-Quan Zhu ◽  
Xu-Hong Wei ◽  
Jun Wang ◽  
Yong-Yong Li ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Sodium Channels ◽  
Dorsal Root ◽  
Interleukin 10 ◽  
Dorsal Root Ganglion Neurons ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels ◽  
Ganglion Neurons

scholarly journals Effect of Methylcobalamin on Voltage-Gated Sodium Channels (Vgscs ) Expression in Neuropathic Painanimal Model

2021 ◽  
Vol 14 (02) ◽  
pp. 1033-1038
Author(s):  
Endang Mutiawati ◽  
K.R.T. Lucas Meliala ◽  
Ginus Partadiredja ◽  
Dhirgo Adji ◽  
Raden Wasito
Keyword(s):  
Neuropathic Pain ◽  
Sodium Channels ◽  
Mechanical Allodynia ◽  
Spinal Nerve ◽  
Control Group ◽  
Pain Model ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels ◽  
Neuropathic Pain Model ◽  
First Onset

The objective of this study wasto assess the effect of methylcobalaminonmechanical allodynia and the voltage-gated sodium channels (VGSCs) expression of injured nerves in spinal nerve ligation-induced neuropathic pain model in animals.Three different doses of methylcobalamin were administrated intramuscularly into neuropathic pain rat model, twice a week for 14 weeks. The effect of methylcobalamin on neuropathic pain was assessed using mechanical allodynia (using the von Frey filaments) while its effect on VGSC expression was assessed using immunohistochemistry. ANOVA and independent t-test were employed to compared the effect of methylcobalamin on mechanical allodynia between groups.The size of von Frey filament that induced the first onset of mechanical allodynia was smaller in control group compared to 50µg methylcobalamin group (p=0.013) and methylcobalamin 100µg group (p=0.019). There is a dose–response relationship between methylcobalamin dose and the average duration of mechanical allodynia (43.8, 38.2, 30.6 and 29.6 days for control, 50µg, 100µg, and 150µg methylcobalamin group, respectively) with a significant different observed between control and 150µg methylcobalamin group only (p=0.027). Nerve tissues from all animals within control group expressed VGSC while all nerve tissues from both 100µg, and 150µg methylcobalamin, had no VGCS expression. In conclusion, methylcobalamin is potentially shorten the duration of mechanical allodynia and increase pain threshold in neuropathic pain animal model. These effects might associate with reduction of VGSC expression on the injured neurons.

scholarly journals Exon 11 skipping ofSCN10Acoding for voltage-gated sodium channels in dorsal root ganglia

Channels ◽  
2014 ◽  
Vol 8 (3) ◽  
pp. 210-215 ◽  
Author(s):  
Jana Schirmeyer ◽  
Karol Szafranski ◽  
Enrico Leipold ◽  
Christian Mawrin ◽  
Matthias Platzer ◽  
...  
Keyword(s):  
Sodium Channels ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels ◽  
Exon 11
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