scholarly journals Immunoturbidimetric determination of serum transferrin on a Kone Progress autoanalyser

1989 ◽  
Vol 11 (1) ◽  
pp. 40-41 ◽  
Author(s):  
Luc Cynober ◽  
Jacques Le Boucher ◽  
Jacqueline Giboudeau
Keyword(s):  
Binding Capacity ◽  
Clinical Chemistry ◽  
Total Iron ◽  
Radial Immunodiffusion ◽  
Serum Transferrin ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
French Society ◽  
Iron Binding Capacity

The Kone Progress, a multiparametric discrete analyser, was used to determine serum transferrin with a kit supplied by Kone. Assays recommended by the French Society of Clinical Chemistry were performed in order to assess the suitability of the test. Repeatability was assessed using serum pools with low (L), medium (M) and high (H) concentrations of transferrin. The coeffcients of variation (CV) were 5.4, 3.2 and 2.0% respectively for 30 determinations (within-batch). Reproducibility on 15 consecutive days (between-batch) was also satisfactory (CV for L = 7.3%, M = 6.3% and H = 3.8%). There was no serum-to-serum contamination. Results correlated closely with those obtained using radial immunodiffusion (RID) (r = 0.942) and total iron-binding capacity (r = 0.954)for 90 determinations.Transferrin measurement by immunoturbidimetry on the Kone Progress emerges as a well-suited, rapid and inexpensive alternative to other time-consuming (RID) and sophisticated (laser immunonephelemeter) techniques.

scholarly journals Fully automated measurement of total iron-binding capacity in serum

1997 ◽  
Vol 43 (12) ◽  
pp. 2413-2417 ◽  
Author(s):  
Hachiro Yamanishi ◽  
Shigeki Kimura ◽  
Shigeru Iyama ◽  
Yoshihisa Yamaguchi ◽  
Takehiko Yanagihara
Keyword(s):  
Binding Capacity ◽  
Colorimetric Method ◽  
Total Iron ◽  
Second Step ◽  
Serum Transferrin ◽  
Acidic Ph ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Automated Measurement ◽  
Iron Binding Capacity

Abstract We established a method for fully automated measurement of total iron-binding capacity (TIBC) in serum without separation of the unbound excess iron after saturating serum transferrin. After saturation of serum transferrin with an excess amount of iron (first step), the unbound iron was eliminated by formation of a complex with ferrozine, which was used as a chromogenic reagent (second step). For the TIBC assay, iron dissociated from transferrin by shifting the pH to acidic was reacted with ferrozine, and the increase in the absorbance at 570 nm was measured (third step). Because the iron used as a calibrator, which was added to saturate transferrin, reacted completely with ferrozine in the second step (elimination of unbound iron), the change in the absorbance to generate a calibration factor could not be monitored in the third step. To solve this problem, we used N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid (HEDTA) to complex with the iron added to saturate transferrin in the second step. This made it possible to form an iron–ferrozine complex at acidic pH because iron was dissociated from HEDTA at acidic pH. The within-run CVs of this method were 0.66–2.43% at 17.7–77.0 μmol/L, and the day-to-day CVs were 1.06–1.57% at 29.9–60.4 μmol/L (n = 10). The correlation between the values obtained with this method (y) and those from the direct TIBC assay, which involved removal of unbound iron by ion-exchange resin (x), was: y = 0.963x + 0.29 μmol/L (r = 0.973, Sy|x = 2.83, n = 59), and with the TIBC values calculated from the serum iron concentrations and the unbound iron-binding capacities measured by a direct colorimetric method (x) was: y = 1.01x − 1.06 μmol/L (r = 0.994, Sy|x = 1.66, n = 51).

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