Abstract 3779: Multiomic single cell analysis of normal human bone marrow identifies a unique stem and progenitor population that expands in AML

2020 ◽  
Author(s):  
Asiri Ediriwickrema ◽  
Sreejith Ramakrishnan ◽  
Margaret Nakamoto ◽  
Smita Ghanekar ◽  
Bogdan Luca ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Analysis ◽  
Normal Human ◽  
Normal Human Bone Marrow

FRACTIONATION OF NORMAL HUMAN BONE MARROW ON ALBUMIN GRADIENTS

1974 ◽  
Vol 52 (5) ◽  
pp. 767-778 ◽  
Author(s):  
KA Rickard ◽  
L Dunleavy ◽  
R Brown ◽  
H Kronenberg
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Normal Human Bone Marrow

The effect of vanocomycin and teicoplanin on normal human bone marrow progenitor cells

1992 ◽  
Vol 30 (4) ◽  
pp. 559-560
Author(s):  
R. DE BOCK ◽  
D. VAN BOCKSTAELE ◽  
H. SNOECK ◽  
F. LARDON ◽  
M. PEETERMANS
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Progenitor Cells ◽  
Bone Marrow Progenitor ◽  
Normal Human ◽  
Normal Human Bone Marrow

Activators and inhibitors of fibrinolysis in normal human bone marrow

1995 ◽  
Vol 6 (2) ◽  
pp. 167
Author(s):  
Nicola A. McWilliam ◽  
Nuala A. Booth ◽  
Linda A. Robbie ◽  
Bruce Bennett
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Normal Human Bone Marrow

scholarly journals Fractionation of subsets of BFU-E from normal human bone marrow: responsiveness to erythropoietin, human placental-conditioned medium, or granulocyte-macrophage colony-stimulating factor

Blood ◽  
1988 ◽  
Vol 71 (3) ◽  
pp. 758-765 ◽  
Author(s):  
G Kannourakis ◽  
GR Johnson
Keyword(s):  
Bone Marrow ◽  
Conditioned Medium ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Colony Stimulating Factor ◽  
Normal Human ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor ◽  
Normal Human Bone Marrow

Abstract Normal human bone marrow mononuclear cells were fractionated by differential adherence, immunomagnetic separation, and fluorescence- activated cell sorting (FACS). The resultant fractionated cells were cultured in semisolid medium to monitor the presence of BFU-E, Mix-CFC, and nonerythroid CFC. Two populations of cells were recovered on the basis of binding by the monoclonal antibody (MoAb) RFB-1. One of these populations contained BFU-E that were stimulated only by erythropoietin (Epo), whereas the second population contained BFU-E responsive to Epo, Epo and recombinant human granulocyte-macrophage colony-stimulating factor (rHGM-CSF), or Epo and human placental-conditioned medium (HPCM). Prior enrichment of clonogenic cells by removal of adherent and Leu-M3+ve, Leu-4+ve, Leu-7+ve, B1+ve, WEMG1+ve, and Glycophorin A+ve cells, followed by FACS fractionation on the basis of RFB-1 binding, consistently resulted in recoveries of BFU-E, Mix-CFC, and nonerythroid CFC of greater than 100% (up to 800%). These procedures also resulted in enrichment of up to 200-fold and frequencies of 1:6 for BFU-E, 1:5 for CFC, and 1:130 for Mix-CFC.

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