Electron-Microscopic Studies on the Maturation of Secretory Cells in the Mouse Harderian Gland

1991 ◽  
Vol 140 (4) ◽  
pp. 304-312 ◽  
Author(s):  
K. Shirama ◽  
M. Hokano
Keyword(s):  
Harderian Gland ◽  
Secretory Cells ◽  
Electron Microscopic ◽  
Microscopic Studies

Correlative transmission and high-resolution scanning electron microscopic studies on pituitary cells

1990 ◽  
Vol 48 (3) ◽  
pp. 20-21
Author(s):  
S. Tai
Keyword(s):  
Cell Types ◽  
Depth Of Field ◽  
Secretory Cells ◽  
Specific Cell ◽  
Pituitary Cells ◽  
Electron Microscopic ◽  
3 Dimensional ◽  
Microscopic Studies ◽  
Structure And Activity ◽  
Intracellular Structure

Extensive cytological and histological research, correlated with physiological experimental analysis, have been done on the anterior pituitaries of many different vertebrates which have provided the knowledge to create the concept that specific cell types synthesize, store and release their specific hormones. These hormones are stored in or associated with granules. Nevertheless, there are still many doubts - that need further studies, specially on the ultrastructure and physiology of these endocrine cells during the process of synthesis, transport and secretion, whereas some new methods may provide the information about the intracellular structure and activity in detail.In the present work, ultrastructural study of the hormone-secretory cells of chicken pituitaries have been done by using TEM as well as HR-SEM, to correlate the informations obtained from 2-dimensional TEM micrography with the 3-dimensional SEM topographic images, which have a continous surface with larger depth of field that - offers the adventage to interpretate some intracellular structures which were not possible to see using TEM.

Zymogen Secreting Cells in the Hepatopancreatic Duct of Pagurus Bernhardus (Linnaeus, 1758) and Clibanarius Longitarsus (De Haan, 1849) (Anomura)

Crustaceana ◽  
1995 ◽  
Vol 68 (5) ◽  
pp. 616-628
Author(s):  
C. Manjulatha ◽  
D. Erri Babu
Keyword(s):  
Connective Tissue ◽  
Secretory Activity ◽  
Secretory Cells ◽  
Main Duct ◽  
Zymogen Granules ◽  
Electron Microscopic ◽  
Food Material ◽  
Acidic Polysaccharide ◽  
Microscopic Studies ◽  
Pagurus Bernhardus

AbstractHistological and histochemical observations reveal that there are two clusters of acidic polysaccharide secreting glands at the opening of the mouth into the oesophagus in Pagurus bernhardus and Clibanarius longitarsus. Below these there are structurally similar glands in the connective tissue of the oesophagus. The epithelial cells lining the hepatopancreatic main duct show secretory activity and they secrete acidophilic granular secretions, which are chemically similar to vertebrate pancreatic zymogen granules. Electron microscopic studies reveal the presence of secretory cells in the hepatopancreatic duct and the synthesis of zymogens within these cells. The time of release of these granular secretions synchronizes with the release of the food material into the midgut.

scholarly journals Karyometric and electron microscopic studies of dark and light cells of the adrenal cortex in mammals

2018 ◽  
Vol 7 (3) ◽  
pp. 61-67 ◽  
Author(s):  
A. G. Ul'yanov ◽  
P. M. Torgun
Keyword(s):  
Electron Microscopy ◽  
Adrenal Cortex ◽  
Lipid Droplets ◽  
Adrenal Glands ◽  
Ovis Aries ◽  
Secretory Cells ◽  
Electron Microscopic ◽  
Dark Cells ◽  
Microscopic Studies ◽  
Light Cells

The purpose of this study is a comparative study of dark and light cells of cortical parenchyma of the adrenal glands in mammals using light and electron microscopy. Material and methods. Cariometric and electron microscopic studies of the adrenal glands of 9 mammalian species have been carried out: Vulpes vulpes (fox), Vulpes lagopus (arctic fox), Canis familiaris (dog), Мartes zibellina (sable), Mustela vison (mink), Enhydra lutris (sea ape); Castor fiber (river beaver); Callorhinus ursinus (fur seal); Ovis aries (sheep). Serial paraffin sections were stained with hematoxylin and eosin, iron hematoxylin, Heidenheim azan, the PAS-reaction was used, the color of the trichrome-PAS and the tetrachromium-PAS. RNA was detected by the reaction of Brashe and gallocyanin by Einarsson. For the detection of lipids, frozen sections were stained with Sudan III + IV, Black Sudan. The number of dark and light cells in the cortex of the adrenal mink in different seasons was determined. On electron micrographs, the amount of lipid droplets and mitochondria in the fascicle of the adrenal cortex was counted. Results. In the cortex of the adrenal glands of mammals, dark and light cells are present. Dark cells, as far as accumulation of sudanophilic substances are converted into light cells, which with further accumulation of lipids undergo destructive changes. Also found are the dark dying cells of the adrenal cortex, characterized by densification of the cytoplasm, pycnosis of nuclei, accumulation of yellow-brown pigment. Dark cells have larger nuclei, give more intense reaction to RNA and ketosteroids and are more active secretory cells compared to light adrenocorticocytes. Electron microscopy in dark cells reveals a large number of mitochondria and a significant amount of lipid droplets are present. Light cells contain many lipid inclusions and few mitochondria.

Fine Structural Changes of the Porcine Oviductal Epithelium During Late and Term Pregnancy

1976 ◽  
Vol 34 ◽  
pp. 150-151
Author(s):  
R. K. Nayak ◽  
E. N. Albert
Keyword(s):  
Estrous Cycle ◽  
Early Pregnancy ◽  
Structural Changes ◽  
Ultrastructural Changes ◽  
Secretory Cells ◽  
Corpora Lutea ◽  
Term Pregnancy ◽  
Electron Microscopic ◽  
Uterine Tube ◽  
Microscopic Studies

The characteristic ultrastructural changes in the ciliated and secretory cells of the tubal epithelium of adult gilts during the estrous cycle have been described (1). Earlier light microscopic studies revealed that pregnancy inhibited the cyclic alterations in the tubes of the sow and the epithelium appeared to remain in the state which was found in the second week after ovulation, when the corpora lutea were full grown (2). Nayak and Zimmerman have presented limited electron microscopic studies of the porcine tubal epithelium during early pregnancy (3) . To the best of our knowledge, no electron microscopic studies of the porcine uterine tube epithelium during late and term pregnancy have been reported.

Light and Electron Microscopic Studies of the Seminal Vesicular Glands of the African Straw-coloured Fruit Bat, Eidolon helvum

2020 ◽  
Author(s):  
C.N. Abiaezute ◽  
I.C. Nwaogu ◽  
I.R. Obidike ◽  
U.M. Igwebuike
Keyword(s):  
Abiotic Factors ◽  
Secretory Cells ◽  
Basal Cells ◽  
Reproductive Structure ◽  
Electron Microscopic ◽  
Dense Granules ◽  
Fruit Bat ◽  
Microscopic Studies ◽  
Eidolon Helvum ◽  
Near Threatened

Background: Secretions from the seminal vesicular gland enhance the survival and reproductive functions of the spermatozoa. The order Chiroptera has evolved great diversity in their reproductive structure influenced by location, abiotic factors and availability of food. Eidolon helvum is a near threatened tropical African fruit bat and little is known of its reproductive biology. The aim of this study was to highlight the structure, histochemistry and ultrastructure of the seminal vesicular glands of Eidolon helvum. Methods: The paired seminal vesicular glands of 16 adult male Eidolon helvum were obtained in May during the early rainy season in tropical Nigeria. The glands were evaluated grossly, histological and ultrastructurally.Result: The spirally coiled tubular organs were divided into numerous lobules of tubular-alveolar glandular acini lined by simple cuboidal epithelium made up of basal cells and mono or bi-nucleated cuboidal secretory cells. PAS positive secretions projected from the apical surfaces. Principal secretory cells contained numerous rough endoplasmic reticulum, mitochondria, Golgi complexes, electron lucid secretory vesicles, electron dense granules and lysosomes. The unique merocrine and apocrine secretions contributed to the formation of vaginal plugs.

scholarly journals Light and electron microscopic studies of the Harderian gland in Bilgorajska goose (Anser anser)

2015 ◽  
Vol 66 (3) ◽  
pp. 249-257
Author(s):  
Joanna Klećkowska-Nawrot ◽  
Aleksandra Chęć ◽  
Karolina Goździewska-Harłajczuk ◽  
Renata Nowaczyk ◽  
Karolina Barszcz
Keyword(s):  
Harderian Gland ◽  
Anser Anser ◽  
Electron Microscopic ◽  
Microscopic Studies

Control of steroid synthesis in human fetal adrenals in monolayer culture

1978 ◽  
Vol 56 (6) ◽  
pp. 577-584 ◽  
Author(s):  
Andrew C. Baird ◽  
Gilles Brisson ◽  
Kenneth W. Kan ◽  
W. C. Duguid ◽  
Samuel Solomon
Keyword(s):  
Monolayer Culture ◽  
Secretory Cells ◽  
Electron Microscopic ◽  
Steroid Synthesis ◽  
Adrenal Cells ◽  
Endogenous Production ◽  
Microscopic Studies ◽  
Lag Period ◽  
Cyclic Phosphate ◽  
Stimulation Of

The actions of adrenocorticotrophin 1-39 (ACTH), N6, O2-dibutyryl adenosine-3′, 5′-cyclic phosphate (dbcAMP), and human chorionic gonadotrophin (hCG) on the sulfation of DHA and the synthesis of cortisol by human fetal adrenal cells in monolayer culture have been described. Electron microscopic studies indicate that the cells present in our cultures at the end of 5 days are intact and have many of the characteristics of secretory cells. Sulfation of DHA was strongly inhibited by 11-deoxycorticosterone, testosterone, androsterone, and dihydrotestosterone. Very small effects were observed with progesterone and corticosterone and insignificant effects with estrone, estradiol, and estriol. Androstenedione as well as 17α-hydroxylated corticosteroids showed no inhibition. When used as substrate, C21 Δ4-3-ketosteroids were good precursors of cortisol with 11-deoxycortisol being the best one followed by 17α-hydroxyprogesterone and progesterone. The conversion of progesterone (4 μg/2 ml) to cortisol was fivefold greater than under conditions where no substrate was present (endogenous production). ACTH in the concentration of 5 × 10−4 U/ml added twice daily to the cell cultures for 8 days increased eightfold the sulfation of DHA and increased cortisol synthesis from progesterone 35-fold in 5 days. The addition of dbcAMP (5 × 10−5 M) or hCG used at 2.0 or 0.2 U/ml did not stimulate the sulfation of DHA. A lag period of at least 36 h is required before the stimulation of DHA sulfation or cortisol synthesis from progesterone can be observed. dbcAMP can stimulate the conversion of progesterone, 17α-hydroxyprogesterone, and 11-deoxycortisol to cortisol by the adrenal cells. These data suggest that 11β- and 21 -hydroxylation are stimulated by dbcAMP but 17α-hydroxylation is not altered.

scholarly journals Scanning Electron Microscopic Studies on Uterus of Jaffarabadi Buffalo during Follicular and Luteal Phases of Estrous Cycle

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Vishnudeo Kumar ◽  
K. N. Vyas ◽  
P. H. Tank ◽  
Anil Sharma ◽  
S. H. Talekar
Keyword(s):  
Luteal Phase ◽  
Electron Microscopic Observation ◽  
Hexagonal Structure ◽  
Follicular Phase ◽  
Secretory Cells ◽  
Ciliated Cells ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Scanning Electron

The study was conducted on uterus of 20 adult Jaffrabadi buffaloes. Scanning electron microscopic observation showed that the surface of endometrium was folded. Lining epithelium of horn and body consist of ciliated and nonciliated cells. During follicular phase cells were flat, formed hexagonal structure with numerous microvilli, whereas, cells were narrow and polygonal in shape during luteal phase. More number of well-developed ciliated cells were found in follicular phase as compared to luteal phase. Numerous secretory blabs were observed on surface of secretory cells during luteal phase. The folds in the cervix were narrow and deep. The lining epithelium of cervix had ciliated and nonciliated cells. The cilia were more in cranial part than the caudal part of the cervix and present in the form of bunch, which were overlapping the secretory cells. Ciliated cells were more in number during follicular phase. Cervical glands were distributed on the cervical mucosa and ciliated cells were present around the openings of these glands.

Light and Electron Microscopic Studies of theGerbillus tarabuli(Thomas, 1902) Harderian Gland

2013 ◽  
Vol 30 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Ouanassa Saadi-Brenkia ◽  
Nadia Haniche ◽  
Mounira Bendjelloul
Keyword(s):  
Harderian Gland ◽  
Electron Microscopic ◽  
Microscopic Studies
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