Higher Expression of miR-182 in Cytology Specimens of High-Grade Urothelial Cell Carcinoma: A Potential Diagnostic Marker

Objective: MicroRNAs (miRs) are short noncoding RNA molecules that posttranscriptionally modulate protein expression. There are distinct miR alterations characterizing urothelial cell carcinoma (UCC) of the urinary bladder. Study Design: In this study, we investigate the possibility of using miR as a noninvasive marker in the screening of UCC. The total RNA was extracted from 75 cytology specimens including bladder or renal washings and voided urines. Cases comprise UCC (21 high grade and 6 low grade), 25 normal controls and 23 cases with a history of UCC but negative at the time of testing (negative with a positive history). The expressions of miR-96, miR-182, miR-183, miR-200c, miR-21, miR-141 and miR-30b were determined using quantitative TaqMan real-time PCR. Results and Conclusion: This study shows that the level of miR-182 is higher in cytology specimens from high-grade UCC patients as compared to normal controls. Measuring miR-182 may provide a potential alternative or adjunct approach for screening high-grade UCC.

Download Full-text

ANALYSIS OF THE EXPRESSION OF FAS/CD95 AND HSP70 IN LOW AND HIGH GRADE UROTHELIAL CELL CARCINOMA OF THE BLADDER

Folia Medica Indonesiana ◽

10.20473/fmi.v53i4.7154 ◽

2017 ◽

Vol 53 (4) ◽

pp. 247

Author(s):

Heryanto Heryanto ◽

Etty Hary Kusumastuti ◽

Anny Setijo Rahaju

Keyword(s):

Cell Carcinoma ◽

Transitional Cell ◽

Urothelial Cell ◽

Hsp70 Expression ◽

Low Grade ◽

High Grade ◽

Urothelial Cell Carcinoma ◽

Cross Sectional ◽

Carcinoma Of The Bladder ◽

Spearman Test

Urothelial Cell Carcinoma (UCC), also called transitional cell carcinoma of the bladder, is the most common malignancy in urinary tract. The prognosis of this disease is highly dependent on the histological grading at diagnosis. Fas/CD95 has a role in apoptotic process, whereas HSP70 has an antiapoptotic role. This study aimed to analyze the expression of Fas/CD95 and HSP70 in low grade and high grade urothelial cell carcinoma in the bladder. This was an analytic observational study with cross sectional approach. Population and sample were paraffin blocks of urothelial cell carcinoma in the Laboratory of Anatomic Pathology, Dr. Soetomo Hospital, Surabaya in the period of January 2011-December 2016. Each grading of urothelial cell carcinoma was randomly sampled. Immunohistochemystry with Fas/CD95 and HSP70 were performed. Expression of Fas/CD95 and HSP70 were assessed semiquantitatively. Expression of Fas/CD95 and HSP70 were analyzed using Mann-Whitney test and Spearman test. The results showed there was significant different in expression of Fas/CD95 and HSP70 in low and high grades in urothelial cell carcinoma. There was no significant correlation between the expression of Fas/CD95 and HSP70 in urothelial cell carcinoma. As a conclusion, the role of CD95 and HSP70 expression can be useful as marker for the diagnosis, especially in the determination of the grade of differentiation.

Download Full-text

Reactivation of BK Polyomavirus in Urine Cytology Is Not Associated with Urothelial Cell Carcinoma

Viruses ◽

10.3390/v12121412 ◽

2020 ◽

Vol 12 (12) ◽

pp. 1412

Author(s):

Faisal Klufah ◽

Ghalib Mobaraki ◽

Axel zur Hausen ◽

Iryna V. Samarska

Keyword(s):

Cell Carcinoma ◽

Urine Cytology ◽

Urothelial Cell ◽

The Other ◽

Urothelial Cell Carcinoma ◽

Tissue Samples ◽

Bk Polyomavirus ◽

Ffpe Tissues ◽

Immunosuppressed Patients ◽

History Of

BK polyomavirus (BKPyV) has been associated with some high-grade and special urothelial cell carcinoma (UCC) subtypes in immunosuppressed patients. Here, we evaluated the relationship of BKPyV-positive urine cytology specimens (UCS) with UCC. A large single-institution database was retrospectively searched for UCS positive for decoy cells, suggesting BKPyV infection. These were tested for the presence of BKPyV by PCR and immunohistochemistry (IHC) in urine sediments and formalin-fixed paraffin-embedded (FFPE) tissue samples of UCC. Decoy cells were reported in 30 patients out of the database with 22.867 UCS. Of these 30 patients, 16 (53.3%) had no history of UCC. Six patients out of these 16 had a history of transplantation, 4 had a history of severe chronic medical conditions, and 6 had no chronic disease. The other fourteen patients were diagnosed with either in situ or invasive UCC of the urinary bladder (14/30; 46.6%) prior to the detection of decoy cells in the urine. Nine of these UCC patients received intravesical treatment (BCG or mitomycin) after the first presentation with UCC. However, the clinical data on the treatment of the other five UCC patients was lacking. IHC identified BKPyV-positivity in the urine samples of non-UCC and UCC patients, while no BKPyV positivity was found in FFPE tissues of primary UCCs and metastases. In addition, BKPyV-PCR results revealed the presence of BKPyV DNA in the urine of the UCC cases, yet none in the UCC tissues itself. These data strongly indicate that BKPyV reactivation is not restricted to immunosuppression. It can be found in UCS of the immunocompetent patients and may be related to the intravesical BCG or mitomycin treatment of the UCC patients.

Download Full-text

Case 12.12

Mayo Clinic Body MRI Case Review ◽

10.1093/med/9780199915705.003.0310 ◽

2014 ◽

pp. 587-587

Author(s):

Christine U. Lee ◽

James F. Glockner

Keyword(s):

Cell Carcinoma ◽

Urothelial Cell ◽

Corpus Spongiosum ◽

Urothelial Cell Carcinoma ◽

Corpora Cavernosa ◽

History Of ◽

The Right ◽

T2 Weighted Images

54-year-old man with a history of a prior cystectomy for bladder urothelial cell carcinoma Axial FSE T2-weighted images (Figure 12.12.1) reveal multiple hypointense masses in both corpora cavernosa (more prominent in the left than in the right) and in the base of the corpus spongiosum....

Download Full-text

Impact of Gene Polymorphisms in GAS5 on Urothelial Cell Carcinoma Development and Clinical Characteristics

Diagnostics ◽

10.3390/diagnostics10050260 ◽

2020 ◽

Vol 10 (5) ◽

pp. 260 ◽

Cited By ~ 1

Author(s):

Wei-Chun Weng ◽

Chih-Jung Chen ◽

Pei-Ni Chen ◽

Shian-Shiang Wang ◽

Ming-Ju Hsieh ◽

...

Keyword(s):

Cell Carcinoma ◽

Tumor Size ◽

Noncoding Rna ◽

Cohort Analysis ◽

Critical Role ◽

Growth Arrest ◽

Urothelial Cell ◽

Rank Test ◽

Urothelial Cell Carcinoma ◽

Female Patients

Urothelial cell carcinoma (UCC) is the commonest malignant tumor of the urinary tract and the second most common kidney cancer malignancy. Growth arrest-specific 5 (GAS5), a long noncoding RNA, is encoded by the GAS5 gene and plays a critical role in cellular growth arrest and apoptosis. In the current study, two single nucleotide polymorphisms (SNPs) in the GAS5 gene, rs145204276 and rs55829688, were selected to investigate correlations between these single SNPs and susceptibility to UCC. A total of 430 UCC cases and 860 ethnically matched healthy controls were included. SNP rs145204276 and SNP rs55829688 were determined using a TaqMan genotyping assay. Logistic regression models demonstrated that female patients with UCC carrying the rs145204276 GAS5 Ins/Del or Del/Del genotype had a 3.037-fold higher risk of larger tumor status (95% confidence interval 1.259–7.324) than did rs145204276 wild type (Ins/Ins) carriers (p = 0.011). The Cancer Genome Atlas validation cohort analysis demonstrated that the expression of GAS5 in female patients with bladder urothelial carcinoma (BLCA) with larger tumor size was much lower than that in patients with a smaller tumor size (p = 0.041). Kaplan-Meier curve analysis and the log–rank test revealed that female patients with BLCA and lower GAS5 expression had poorer overall survival than those with higher GAS5 expression. In conclusion, genetic variations in GAS5 rs145204276 may serve as a critical predictor of the clinical status of female patients with UCC.

Download Full-text