scholarly journals Orange/Red Fluorescence of Active Caries by Retrospective Quantitative Light-Induced Fluorescence Image Analysis

2016 ◽  
Vol 50 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Grace Felix Gomez ◽  
George J. Eckert ◽  
Andrea Ferreira Zandona
Keyword(s):  
Image Analysis ◽  
Mixed Model ◽  
Assessment System ◽  
Caries Detection ◽  
Percentage Increase ◽  
Caries Activity ◽  
Red Fluorescence ◽  
Mixed Model Anova ◽  
Retrospective Clinical Study ◽  
Final Observation

This retrospective clinical study determined the association of caries activity and orange/red fluorescence on quantitative light-induced fluorescence (QLF) images of surfaces that progressed to cavitation, as determined by clinical visual examination. A random sample of QLF images from 565 children (5-13 years) previously enrolled in a longitudinal study was selected. Buccal, lingual and occlusal surface images obtained after professional brushing at baseline and every 4 months over a 4-year period were analyzed for red fluorescence. Surfaces that progressed (n = 224) to cavitation according to the International Caries Detection and Assessment System (ICDAS 0/1/2/3/4 to 5/6 or filling), and surfaces that did not progress (n = 486) were included. QA2 image analysis software outputs the percentage increase of the red/green components as ΔR and area of ΔR (areaΔR) at different thresholds. Mixed-model ANOVA was used to compare progressive and nonprogressive surfaces to account for correlations of red fluorescence (ΔR and areaΔR) between surfaces within a subject. The first analysis used the first observation for each surface or the first available visit if the surface was unerupted (baseline), while the second analysis used the last observation prior to cavitation for surfaces that progressed and the last observation for surfaces that did not progress (final). There was a significant (p < 0.05) association between red fluorescence and progression to cavitation at thresholds ΔR0, ΔR10, ΔR20, ΔR60, ΔR70, ΔR80, ΔR90 and ΔRmax at baseline and for ΔR0 and ΔR10 at the final observation. Quantification of orange/red fluorescence may help to identify lesions that progress to cavitation. Future studies identifying microbiological factors causing orange/ red fluorescence and its caries activity are indicated.

scholarly journals Influence of Examiner Experience on Clinical Performance of Visual Inspection in Detecting and Assessing the Activity Status of Caries Lesions

2013 ◽  
Vol 38 (6) ◽  
pp. 583-590 ◽  
Author(s):  
T Gimenez ◽  
DG Bittar ◽  
C Piovesan ◽  
CAB Guglielmi ◽  
KY Fujimoto ◽  
...  
Keyword(s):  
Clinical Features ◽  
Visual Inspection ◽  
Dental Treatment ◽  
Pediatric Dentistry ◽  
Dental Students ◽  
Assessment System ◽  
Caries Detection ◽  
Caries Activity ◽  
Caries Lesions ◽  
Different Levels

SUMMARYOur hypothesis was that a method of caries activity evaluation based on the clinical features of the lesions would be less time consuming but more influenced by the examiner's experience than the scoring system used in association with the International Caries Detection and Assessment System (ICDAS). Thus, the aim of this study was to evaluate the performance of three groups of examiners with different levels of experience using two different methods to assess the activity status of caries lesions by visual inspection. A cross-sectional study in a dental office setting was performed selecting 18 children, aged three to eight years, who had sought dental treatment at a dental school. Examinations to detect caries lesions were performed using visual inspection by six examiners with different levels of experience: two undergraduate dental students, two specialists in pediatric dentistry, and two graduate students. The examiners used ICDAS and two different methods to assess caries activity: using an additional score system or considering the examination of clinical features. Two benchmark examiners examined the children in a joint session, and their consensus was considered to be the reference standard. The sensitivity, specificity, and reproducibility were calculated for different thresholds: all, cavitated, and active caries lesions. Multilevel analyses were performed to compare the different methods and examiners. No differences were observed among the examiners, either in detecting all lesions and cavitated lesions or regarding the activity assessment. The methods of assessing activity status performed similarly, but the time spent on examinations was shorter for the method evaluating clinical features. In conclusion, the experience of examiners does not significantly influence the performance of visual inspection, and both methods of assessing activity status result in similar diagnostic accuracy.

scholarly journals Porphyrin and Pentosidine Involvement in the Red Fluorescence of Enamel and Dentin Caries

2016 ◽  
Vol 5 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Amel Slimani ◽  
Fares Nouioua ◽  
Ivan Panayotov ◽  
Nicolas Giraudeau ◽  
Kitamura Chiaki ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fluorescence Spectroscopy ◽  
Single Photon ◽  
Early Stage ◽  
Assessment System ◽  
Caries Detection ◽  
Color Analysis ◽  
Red Fluorescence ◽  
Micro Raman Spectroscopy ◽  
Dentin Caries

ABSTRACT Proper treatment of dental caries demands detection of carious lesions at an early stage and a minimal invasive cavity preparation to preserve the maximum tooth structure. Various devices use fluorescence for caries detection via recording the red fluorescence generated by dentin caries under illumination. The aim of this study was to evaluate the porphyrin and pentosidine involvement in the red fluorescence observed in enamel and dentin caries when illuminated with the Soprolife® camera (Sopro, Acteon Group, La Ciotat, France) and Vistacam® camera (Dürr Dental AG, Bietigheim-Bissingen, Germany). Three techniques were used: single photon fluorescence spectroscopy, micro-Raman spectroscopy, and color analysis with ImajeJ software. Cross-sections of human teeth, scored from 0–6 with the International Caries Detection and Assessment System (ICDAS), were examined by fluorescence microscopy. Teeth spectra of each ICDAS score were compared with those of protoporphyrin IX (PpIX), porphyrin I, and pentosidine solutions. A specific confocal Raman microscopy analysis was realized and a Red–Green–Blue model analysis of Soprolife® images was performed using ImageJ software to compare the color variations on ICDAS score 1 and 2. Fluorescence spectroscopy and micro-Raman spectroscopy revealed the presence of PpIX in carious enamel and dentin. The clinical relevance of this experimentation was that the increased knowledge of the fluorescence aids for caries detection could improve the preventive approach, thus reducing the operative one. How to cite this article Slimani A, Nouioua F, Panayotov I, Giraudeau N, Chiaki K, Shinji Y, Cloitre T, Levallois B, Gergely C, Cuisinier F, Tassery H. Porphyrin and Pentosidine Involvements in the Red Fluorescence of Enamel and Dentin Caries. Int J Experiment Dent Sci 2016;5(1):1-10.

scholarly journals Dental caries among Finnish teenagers participating in physical activity and diet intervention: association with anthropometrics and behavioural factors

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mirja Methuen ◽  
Sofia Kauppinen ◽  
Anna Liisa Suominen ◽  
Aino-Maija Eloranta ◽  
Juuso Väistö ◽  
...  
Keyword(s):  
Physical Activity ◽  
Dental Caries ◽  
Assessment System ◽  
Carbohydrate Intake ◽  
Caries Detection ◽  
Caries Experience ◽  
Food Record ◽  
Fat Percentage ◽  
Teenage Girls ◽  
Behavioural Factors

Abstract Background An association between childhood anthropometric measurements and dental caries is conflicting. The prevalence and severity of dental caries and its association with anthropometric and behavioural factors, were investigated among Finnish teenagers. Methods The study sample comprised 202 15–17-year-old participants in the Physical Activity and Nutrition in Children (PANIC) Study. Dental caries findings were recorded using International Caries Detection and Assessment System (ICDAS) criteria, including activity estimation; numbers of decayed teeth (DT) and decayed, missing and filled teeth (DMFT) were recorded. Body weight, height and waist circumference were measured and respective body mass index (BMI) was calculated. Body fat percentage was assessed by dual-energy X-ray absorptiometry. Health-related behaviours and consumption of food and drinks were assessed using questionnaires, and intake of nutrients using a 4-day food record. Results Mean DMFT for all the participants was 2.4 (SD = 2.9), DT 0.6 (SD = 1.3), and 36% had DMFT = 0. No difference between genders was observed. In bivariate analyses, use of sugar-sweetened beverages (SSB) three times or less per week and not having used snuff associated significantly, whereas higher carbohydrate intake (E%), toothbrushing less often than twice a day and higher caries experience at baseline almost significantly with DT > 0. In adjusted regression analyses, frequent use of SSB and higher carbohydrate intake increased the odds for DT > 0. Additionally, higher carbohydrate intake (E%) and infrequent tooth brushing significantly associated with a higher number of DT. Conclusion Caries prevalence is still low and similar in Finnish teenage girls and boys. Behavioural factors are, but anthropometric factors are not associated with dental caries.

scholarly journals 109 Phytate supplementation has minimal impact on mineral digestibility in horses

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 89-90
Author(s):  
Katy Brinkley-Bissinger ◽  
Laura M Cersosimo ◽  
Kathleen E Sullivan ◽  
Shannon E Livingston ◽  
Jill M Bobel ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Mixed Model ◽  
Divalent Cations ◽  
Basal Diet ◽  
Minimal Impact ◽  
Inductively Coupled ◽  
Mixed Model Anova ◽  
Microbial Phytase ◽  
And Performance ◽  
Inorganic Mineral

Abstract Phosphorus in equine rations is supplied by inorganic mineral fortification or naturally-occurring P in forages and grains. Up to 70% of P in plant material is bound to phytate. In monogastrics like poultry and swine, phytate can reduce absorption of P and divalent cations, but the extent that this occurs in horses is unknown. This study tested the hypothesis that phytate decreases mineral digestibility in horses. Six mature Quarter Horse geldings (mean ± SE, 586 ± 19 kg, 10 ± 1.5 y) were randomly assigned to two treatments applied in a cross-over design: IP6 (Ca-Mg-phytate isolated from rice bran fed at 15 mg phytic acid/kg BW) or CON (equivalent Ca, Mg and P from inorganic minerals to match intake from IP6). The level of phytate added represented an amount present in grain-rich rations typically fed to broodmares, growing horses and performance athletes. Supplements were added to a basal diet (1.75% BW, DM basis) consisting of 75% timothy hay and 25% roughage-based concentrate. Each 14-d period had an 11-d treatment adaptation followed by a 3-d total fecal collection. After acid digestion, P was determined colorimetrically and other minerals were determined by inductively coupled plasma spectrometry. Data were analyzed using mixed model ANOVA. Intakes of Ca, P, Mg, Zn, Cu and Fe were similar between treatments (140, 72, 40, 1.05, 0.24, and 1.78 mg/kg BW respectively). Apparent P digestibility (18.8 and 17%, SEM 1.9; P = 0.41) and estimates of true P digestibility (32.8 and 30.8%, SEM 1.9; P = 0.39) were similar between CON and IP6. Apparent digestibilities of other minerals were also not affected by IP6 supplementation. Findings suggest horses have sufficient microbial phytase activity in the gastrointestinal tract to mitigate impacts of dietary phytate. Higher levels or different forms of phytate and marginal mineral intake may yield different results.

scholarly journals Self-assembling Peptide P11-4 and Fluoride for Regenerating Enamel

2017 ◽  
Vol 97 (2) ◽  
pp. 148-154 ◽  
Author(s):  
M. Alkilzy ◽  
A. Tarabaih ◽  
R.M. Santamaria ◽  
C.H. Splieth
Keyword(s):  
Odds Ratio ◽  
Test Group ◽  
Assessment System ◽  
Laser Fluorescence ◽  
Fluoride Varnish ◽  
Caries Detection ◽  
Control Group ◽  
Analog Scale ◽  
Self Assembling ◽  
Carious Lesions

Regenerative medicine-based approaches for caries treatment focus on biomimetic remineralization of initial carious lesions as a minimal invasive therapy. In vitro, self-assembling peptide P11-4 enhances remineralization of early carious lesions. To investigate the safety and clinical efficacy of P11-4 for treatment of initial caries, a randomized controlled single-blind study was conducted on children aged >5 y with visible active early caries on erupting permanent molars. Subjects were randomized to either the test group (P11-4 + fluoride varnish) or control group (fluoride varnish alone). Caries were assessed at baseline and at 3 and 6 mo posttreatment per laser fluorescence, a visual analog scale, the International Caries Detection and Assessment System, and Nyvad caries activity criteria. Intention-to-treat analyses were performed, and safety and clinical feasibility of the treatment approaches were assessed. Compared with the control group, the test group showed clinically and statistically significant improvement in all outcomes at 3 and 6 mo. The laser fluorescence readings (odds ratio = 3.5, P = 0.015) and visual analog scale scores (odds ratio = 7.9, P < .0001) were significantly lower for the test group, and they showed regression in the International Caries Detection and Assessment System caries index (odds ratio = 5.1, P = 0.018) and conversion from active to inactive lesions according to Nyvad criteria (odds ratio = 12.2, P < 0.0001). No adverse events occurred. The biomimetic mineralization facilitated by P11-4 in combination with fluoride application is a simple, safe, and effective noninvasive treatment for early carious lesions that is superior to the presently used gold standard of fluoride alone. By regenerating enamel tissue and preventing lesion progression, this novel approach could change clinical dental practice from a restorative to a therapeutic approach. This could avoid additional loss of healthy hard tissue during invasive restorative treatments, potentially enabling longer tooth life and thereby lowering long-term health costs ( ClinicalTrials.gov NCT02724592).

scholarly journals Multidisciplinary Evaluation of Pacifier Removal on Oro-Dentofacial Structures: A Controlled Clinical Trial

2021 ◽  
Vol 9 ◽  
Author(s):  
Kelly Guedes de Oliveira Scudine ◽  
Camila Nobre de Freitas ◽  
Kizzy Silva Germano Nascimento de Moraes ◽  
Silvana Bommarito ◽  
Rosana de Fátima Possobon ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Mixed Model ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Functional Changes ◽  
Mixed Model Anova ◽  
And Function ◽  
Clinical Trials Registry ◽  
Facial Anthropometry ◽  
Over Time

It is well recognized that pacifier habit leads to occlusal and orofacial functional changes in children. However, the effects of the interruption of prolonged pacifier habit on the development of the dento-facial complex has not yet been fully characterized. Thus, the aim of this study was to investigate the influence of pacifier removal on aspects of oro-dentofacial morphology and function in preschool children. For that, a pacifier group (n = 28) and a control group (n = 32) of 4-year-old children with and without pacifier habit, respectively, were followed up by a group of dentists and speech therapists at baseline, 6 and 12 months after habit removal. Bite force and lip pressure were assessed using digital systems, and the evaluation of breathing and speech functions was performed using validated protocols, together with the measurements of dental casts and facial anthropometry. The Two-way mixed model ANOVA was used in data analysis. After 12 months, a decrease in malocclusion frequency was observed in pacifier group. Additionally, a change over time was observed in facial, intermolar and palate depth measurements, as well in bite and lip forces and speech function scores, increasing in both groups (p &lt; 0.01). The upper and lower intercanine widths and breathing scores differed between groups at baseline and changed over time reducing the differences. The presence of speech distortions was more frequent in the pacifier group at baseline and decreased over time (p &lt; 0.05). The interruption of pacifier habit improved the maxillary and mandibular intercanine widths, as well as the breathing and speech functions, overcoming the oro-dentofacial changes found.Trial Registration: This clinical trial was registered in the Brazilian Clinical Trials Registry (ReBEC; http://www.ensaiosclinicos.gov.br/), protocol no. RBR-728MJ2.

Sign in / Sign up

Export Citation Format

Share Document