Perfusion-Decellularized Larynx as a Natural 3D Scaffold in a Rabbit Model

ORL ◽  
2021 ◽  
pp. 1-8
Author(s):  
Jun-Ook Park ◽  
Hee-Young Park ◽  
Sung-Chan Shin ◽  
Dong-Hyun Lee ◽  
Byung-Joo Lee
Keyword(s):  
Histological Analysis ◽  
Rabbit Model ◽  
Control Group ◽  
3D Scaffold ◽  
3 Dimensional ◽  
Intact Cartilage ◽  
Total Dna ◽  
The Mean ◽  
Cellular Components ◽  
Dna Amounts

<b><i>Introduction:</i></b> Decellularized larynges could be used as scaffolds to regenerate the larynx. The purpose of this study was to establish a perfusion decellularization protocol to produce a 3-dimensional whole laryngeal extracellular matrix (ECM) scaffold in a rabbit model. <b><i>Methods:</i></b> The larynges of 20 rabbits assigned to the study group were harvested and decellularized using a perfusion decellularization protocol, while the larynges of 10 rabbits in the control group were harvested and untreated. Macroscopic and microscopic morphological analyses, a molecular analysis, a cellular content analysis, and scanning electron microscopy were performed. <b><i>Results:</i></b> A histological analysis showed the absence of cellular components, the presence of the ECM, and an intact cartilage structure filled with chondrocytes. The mean total DNA amounts of the native larynx, decellularized larynx, and decellularized cartilage-free larynx were 1,826.40, 434.70, and 41.40 μg/µL, respectively; those for the decellularized larynx and decellularized cartilage-free larynx were significantly lower (<i>p</i> &#x3c; 0.001 and <i>p</i> &#x3c; 0.001, respectively). The total amount of DNA in the decellularized sample was significantly lower compared to that in the native sample, at 57.2% in cartilage (<i>p</i> &#x3c; 0.001), 2.4% in the thyroid gland (<i>p</i> &#x3c; 0.001), 2.7% in muscle (<i>p</i> &#x3c; 0.001), 1.6% in vessels (<i>p</i> &#x3c; 0.001), and 4.8% in the vocal cords (<i>p</i> &#x3c; 0.001). <b><i>Conclusion:</i></b> Our perfusion decellularization protocol is feasible and reproducible to produce a 3-dimensional whole laryngeal ECM scaffold in a rabbit.

scholarly journals Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Nelson Bergonse Neto ◽  
Lianna Ferrari Jorge ◽  
Julio C. Francisco ◽  
Bruna Olandoski Erbano ◽  
Barbara Evelin Gonçalves Barboza ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Histological Analysis ◽  
Rabbit Model ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Surgical Tracheostomy ◽  
Porcine Small Intestinal Submucosa ◽  
Tracheal Tissue ◽  
Small Intestinal ◽  
Intestinal Submucosa

Background. Surgical correction of tracheal defects is a complex procedure when the gold standard treatment with primary end-to-end anastomosis is not possible. An alternative treatment may be the use of porcine small intestinal submucosa (SIS). It has been used as graft material for bioengineering applications and to promote tissue regeneration. The aim of this study was to evaluate whether SIS grafts improved tracheal tissue regeneration in a rabbit model of experimental tracheostomy. Methods. Sixteen rabbits were randomized into two groups. Animals in the control group underwent only surgical tracheostomy, while animals in the SIS group underwent surgical tracheostomy with an SIS graft covering the defect. We examined tissues at the site of tracheostomy 60 days after surgery using histological analysis with hematoxylin and eosin (H&E) staining and analyzed the perimeter and area of the defect with Image-Pro® PLUS 4.5 (Media Cybernetics). Results. The average perimeter and area of the defects were smaller by 15.3% (p=0.034) and 21.8% (p=0.151), respectively, in the SIS group than in the control group. Histological analysis revealed immature cartilage, pseudostratified ciliated epithelium, and connective tissue in 54.5% (p=0.018) of the SIS group, while no cartilaginous regeneration was observed in the control group. Conclusions. Although tracheal SIS engraftment could not prevent stenosis in a rabbit model of tracheal injury, it produced some remarkable changes, efficiently facilitating neovascularization, reepithelialization, and neoformation of immature cartilage.

scholarly journals The Effect of Subconjunctival Bevacizumab on Angiogenesis in Rabbit Model

2020 ◽  
Vol 55 (4) ◽  
pp. 290
Author(s):  
Nurwasis Nurwasis ◽  
Diana Yuliawati ◽  
Evelyn Komaratih ◽  
Heriyawati Heriyawati
Keyword(s):  
Blood Vessels ◽  
Healing Process ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Histopathological Analysis ◽  
Subconjunctival Injection ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

The wound healing process is major cause of glaucoma surgery failure and enhances the incapability of controlling the IOP. This aim of study was to evaluate the angiogenesis effect of Bevacizumab injection on the amount and density of blood vessels in the rabbit model after trabeculectomy. It was a true experimental study using 16 eyes of 16 New Zealand White Rabbit (Oryctolagus cuniculus) that divided into two groups. The control group was treated with 0.05 ml of Balanced Saline Solution (BSS). The experimental group was treated with subconjunctival injection of 1.25 mg Bevacizumab in 0.05 BSS. All rabbit was sacrificed, and the eye was enucleated. Thus, the bleb area was dissected after 14 days. Histopathological analysis was performed to evaluate the amount and density of blood vessels. The mean amount of blood vessels in the control group was 22.63 ± 11.02, and the experimental group was 14,75 ± 4.92 (p=0.043). The mean of blood vessel density in the control group was 19.10 ± 1.69 %, and the experimental group was 16.53 ± 2.90 % (p=0.029)%. There was a statistically significant difference between the two groups (p<0.05). Subconjunctival bevacizumab in the rabbit model reduces the amount and density of blood vessels compared with subconjunctival BSS injection.

scholarly journals Preoperative Complete Patellofemoral Dislocation in Extension Predicts an Inferior Clinical Outcome After Medial Patellofemoral Ligament Reconstruction in Patients With Recurrent Patellar Dislocation

2020 ◽  
Vol 8 (7) ◽  
pp. 232596712093898
Author(s):  
ZhiJun Zhang ◽  
GuanYang Song ◽  
QianKun Ni ◽  
Tong Zheng ◽  
Yanwei Cao ◽  
...  
Keyword(s):  
Patellar Dislocation ◽  
Trochlear Dysplasia ◽  
Tibial Tubercle ◽  
Control Group ◽  
3 Dimensional ◽  
Patellar Maltracking ◽  
Patient Reported ◽  
The Mean ◽  
Redislocation Rate

Background: Habitual patellar dislocation in extension (HPD-E) is a distinctive subtype of recurrent patellar dislocation (RPD); HPD-E represents the most severe type of patellar maltracking in RPD. It has been reported that the presence of preoperative patellar maltracking is associated with a worse clinical outcome after medial patellofemoral ligament (MPFL) reconstruction (MPFL-R). Purpose: To describe the radiological characteristics of HPD-E and to compare clinical outcomes after MPFL-R among patients with and without preoperative HPD-E. Study Design: Cohort study; Level of evidence, 3. Methods: From January 2012 to December 2015, a total of 230 consecutive patients (246 knees) with RPD were treated with MPFL-R alone or combined with tibial tubercle osteotomy. Among them, 28 patients diagnosed with HPD-E by preoperative 3-dimensional computed tomography (CT; HPD-E group) were matched in a 1:1 fashion to 28 control participants who did not show HPD-E (control group). Routine radiography and CT were performed to evaluate patellar height, trochlear dysplasia, tibial tubercle–trochlear groove distance, and torsional deformities. The mean patellar laxity index and lateral patellar translation assessed with stress radiography were measured preoperatively and postoperatively to quantify MPFL laxity. At minimum 2-year follow-up, patient-reported outcomes (Kujala, Lysholm, and Tegner scores), patellar maltracking, and redislocation rates were compared between the HPD-E and control groups. Results: The radiological characteristics of the HPD-E group were as follows: 89% (25/28) of patients had severe trochlear dysplasia (Dejour type B or D), and the mean femoral anteversion angle was 35.5° ± 4.7°. At the final follow-up, the HPD-E group had a significantly lower Kujala score (76.2 vs 84.5, respectively; P = .001), Lysholm score (75.4 vs 86.6, respectively; P < .001), and Tegner score (4.1 vs 5.8, respectively; P = .021) compared with the control group. The postoperative patellar laxity index (43% vs 19%, respectively; P < .001) and redislocation rate (25% vs 0%, respectively; P = .01) were significantly higher in the HPD-E group than in the control group. Conclusion: Preoperative 3-dimensional CT is a reliable method of identfying patients with HPD-E. Treatment of HPD-E by MPFL-R alone or combined with tibial tubercle osteotomy resulted in a higher redislocation rate, more severe MPFL residual laxity, and lower patient-reported outcome scores compared with patients without HPD-E who underwent MPFL-R.

scholarly journals In vivo evaluation of a novel nanocomposite porous 3D scaffold in a rabbit model: histological analysis

2017 ◽  
Vol Volume 12 ◽  
pp. 8587-8598 ◽  
Author(s):  
Saffanah Mahmood ◽  
Abdul Razak Intan-Shameha ◽  
Mustafa Ghaji ◽  
Loqman Mohamad Yusof ◽  
Zaid Mahmood ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Rabbit Model ◽  
3D Scaffold ◽  
In Vivo Evaluation

Statins are not associated with short-term improved aneurysm healing in a rabbit model of unruptured aneurysms

2016 ◽  
Vol 9 (2) ◽  
pp. 200-203 ◽  
Author(s):  
Waleed Brinjikji ◽  
Ding Yong Hong ◽  
Daying Dai ◽  
Dana J Schroeder ◽  
David F Kallmes ◽  
...  
Keyword(s):  
Coil Embolization ◽  
Rabbit Model ◽  
Control Group ◽  
Mesenchymal Progenitor Cells ◽  
Unruptured Aneurysms ◽  
Unruptured Intracranial Aneurysms ◽  
Platinum Coil ◽  
Aneurysm Occlusion ◽  
The Mean ◽  
And Control

BackgroundOwing to their anti-inflammatory effects and ability to stimulate production of extracellular matrix and chemotactic migration of mesenchymal progenitor cells, statins could potentially improve aneurysm healing after endovascular treatment.ObjectiveTo test the hypothesis that systemic administration of simvastatin would improve aneurysm healing in a rabbit model of unruptured intracranial aneurysms.MethodsExperimental aneurysms were created in female rabbits and were embolized with platinum coils. Six rabbits served as controls and six rabbits received oral administration of simvastatin. Digital subtraction angiography was used to evaluate stability after embolization. Subjects were euthanized 4 weeks after coil embolization. Histologic samples were examined with a grading system (0–12) based on neck and dome features. Aneurysm occlusion data were compared using a Student t test.ResultsNo significant differences in the mean aneurysm size were found between groups. No coil compaction occurred in either group. All aneurysms in both the statin and control groups showed stable occlusion. There were no significant differences in the histologic grade of occlusion in either group (statin group 2.6±0.8 vs control group 2.7±3.2, p=0.94).ConclusionsSystemic statin administration after platinum coil embolization of unruptured aneurysms in a rabbit model does not improve aneurysm occlusion rates at 4 weeks.

scholarly journals Cranial hyperthermia resulting from decreased parotid gland salivation induced by ischemic degeneration of glossopharyngeal network in subarachnoid hemorrhage

2021 ◽  
Vol 8 (3) ◽  
pp. 150-154
Author(s):  
Metehan Eseoğlu ◽  
İlhan Yılmaz ◽  
Osman Tanrıverdi ◽  
Nazan Aydın ◽  
Cengiz Öztürk ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Rabbit Model ◽  
Salivary Secretion ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Parotid Glands ◽  
Mean Values ◽  
Parasympathetic Nerves ◽  
Saliva Secretion ◽  
The Mean

Objective: We speculated that subarachnoid hemorrhage (SAH) induces ischemic lesions in the cranial parasympathetic nerves, which may decrease saliva secretion and lead to hyperthermia. We tested this hypothesis by examining histologic features of parotid glands and glossopharyngeal nerves (GPNs) in a rabbit model of SAH. Material and Methods: Rabbits (n = 25) were divided into control (n = 5), sham (n = 5), and SAH (n = 15) groups. Animals in the sham and SAH groups were examined over a 3-week period before sacrifice. Salivation score (SC) was determined by measuring the mean wetted area of an orally inserted cotton ball. Sections of parotid glands and intracranial and intraparotideal branches of the GPNs were stained with hematoxylin and eosin and SAH-induced damage was analyzed by terminal deoxynucleotidyl transferase dUTP nick end labeling of apoptotic cells. Specimens were stereologically examined to determine saliva-filled total vesicle volume (TVV) per cubic meter; vasospasm index (VSI) based on wall/lumen ratio of parotid glands arteries, and degenerated neuron density (DND) of glossopharyngeal ganglia. Results: The mean oral temperature was 36.9°C. In the control group, mean values were as follows: SC, 46±8 mm2; DND, 19±4/mm3; VSI, 1.065±0.049; and TVV, (780±1187) × 106/µm3. In the sham group, mean values were as follows: SC, 31±6 mm2; DND, 98±23/mm3; VSI, 1.67±0.32; and TVV, (617±110) × 106/µm3. In the low hyperthermia SAH group, mean values were as follows: SC, 16±5 mm2; DND, 1520±261/mm3; VSI, 2.12±0.21, and TVV, (314±98) × 106/µm3. In the high hypothermia SAH group, mean values were as follows: SC, 9±2 mm2; DND, 3210±912/mm3; VSI, 3.18±0.30; and TVV, (432±99) × 106/µm3. Conclusions: Decreased salivary secretion due to secretory gland atrophy originated from ischemia-induced GPN network degeneration at the brainstem, which may be responsible for cranial hyperthermia following SAH.

scholarly journals The Effect of Subconjunctival Bevacizumab on Angiogenesis in Rabbit Model

2021 ◽  
Vol 55 (4) ◽  
pp. 290
Author(s):  
Nurwasis Nurwasis ◽  
Diana Yuliawati ◽  
Evelyn Komaratih ◽  
Heriyawati Heriyawati
Keyword(s):  
Blood Vessels ◽  
Healing Process ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Histopathological Analysis ◽  
Subconjunctival Injection ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

The wound healing process is major cause of glaucoma surgery failure and enhances the incapability of controlling the IOP. This aim of study was to evaluate the angiogenesis effect of Bevacizumab injection on the amount and density of blood vessels in the rabbit model after trabeculectomy. It was a true experimental study using 16 eyes of 16 New Zealand White Rabbit (Oryctolagus cuniculus) that divided into two groups. The control group was treated with 0.05 ml of Balanced Saline Solution (BSS). The experimental group was treated with subconjunctival injection of 1.25 mg Bevacizumab in 0.05 BSS. All rabbit was sacrificed, and the eye was enucleated. Thus, the bleb area was dissected after 14 days. Histopathological analysis was performed to evaluate the amount and density of blood vessels. The mean amount of blood vessels in the control group was 22.63 ± 11.02, and the experimental group was 14,75 ± 4.92 (p=0.043). The mean of blood vessel density in the control group was 19.10 ± 1.69 %, and the experimental group was 16.53 ± 2.90 % (p=0.029)%. There was a statistically significant difference between the two groups (p<0.05). Subconjunctival bevacizumab in the rabbit model reduces the amount and density of blood vessels compared with subconjunctival BSS injection.

Protective effects of anionic nanoliposomes on hyperlipidemia and atherosclerosis progression in a rabbit model

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
A.A Momtazi-Borojeni ◽  
E Abdollahi ◽  
M.R Jaafari ◽  
M Banach ◽  
A.A Sahebkar
Keyword(s):  
White Male ◽  
Rabbit Model ◽  
Plasma Lipoproteins ◽  
Control Group ◽  
Chow Diet ◽  
Protective Effects ◽  
Lipid Film ◽  
Atherosclerosis Progression ◽  
The Mean ◽  
And Control

Abstract Background and purpose Anionic nanoliposomes can regulate lipid metabolism by interacting with plasma lipoproteins. Here, we aimed to investigate the protective effects of anionic nanoliposomes against hyperlipidemia and atherosclerosis in the rabbit fed a high-fat diet (HFD) recapitulating human hyperlipidemia and atherosclerosis. Methods To prepare anionic nanoliposome formulations [containing hydrogenated soy phosphatidylcholine (HSPC) and 1,2-distearoyl-sn-glycero-3- phosphoglycerol (DSPG)] and [containing 1,2-Dimyristoyl-sn-glycero-3-phosphorylcholine (DMPC) and 1,2-Dimyristoyl-sn-glycero-3-phosphorylcholine (DMPG)], lipid film hydration method followed by extrusion. Physical properties of the prepared liposome formulations, including particle size (diameter, nm), polydispersity index (PDI), and surface charge, were determined using dynamic light scattering (DLS) technique. New Zealand white male rabbits (4–6 months old, weight 2±0.15 kg) were fed on a regimen chow diet containing 15% fat and 0.5% cholesterol for 8 weeks. To evaluate the protective effects of anionic nanoliposomes, treatment was primed at week 4 on animals equally divided into three groups, including rabbits receiving HSPC/DSPG formulation (DSPG group) and DMPG/DMPC formulation (DMPG group) at the dose of 100 mM/kg via intravenous administration for 4 weeks with a weekly interval, as well as control group receiving saline buffer at a same administration route. Results Anionic nanoliposomal formulations were found to significantly regulate plasma levels of lipid parameters in the rabbit model of atherosclerosis by reducing TC (by 58±9%; 95% CI: 46–70% [HSPC/DSPG] and 37 5%; 95% I: 31–44% [DMPG/DMPC]), LDL-C (by 64 6%: 95% CI: 56–72% [HSPC/DSPG] and 53±10%; 95% CI: 37–67% [DMPC/DMPG], TG (by 62±3%; 95% CI: 58–67% [HSPC/DSPG] and 58±2%; 95% CI: 55–61% [DMPC/DMPG], and elevating HDL-C (by 67±28%; 95% CI: 32–102% [HSPC/DSPG] and 35±19%; 95% CI: 11–60% [DMPC/DMPG]), when compared to the control group at the end of study. Lesion severity in treated rabbits was found to be significantly (p&lt;0.05) lower compared to control rabbits, for which medians (interquartile range) of plaque grades in DSPG, DMPG and control groups were 1 [1–2.5], 2 [1.5–2.5], and 4 [3–4], respectively. Of note, the mean±SD of plaque size in DSPG (54±24 μm; 95% CI: 24–83 μm) and DMPG (92±24 μm; 95% CI: 62–121 μm) groups were shown to be significantly (p&lt;0.05) smaller (−63±17% and 36±16%, respectively) than those in control group (144±28 μm, 95% CI: 109–179 μm). The mean±SD of IMT in DSPG (0.54±0.02 fold; 95% CI: 0.3–0.8 fold) and DMPG (0.96±0.1 fold; 95% CI: 0.8–1.1 fold) groups were shown to be significantly (p&lt;0.05) smaller (−55±17% and −20±4%, respectively) than those in control group (1.2±0.2 fold; 95% CI: 0.9–1.4 fold) (Figure). Conclusion Aninonic nanoliposomes containing [HSPC/DSPG] and [DMPC/DMPG] could significantly protect against hyperlipidemia and atherosclerosis plaque progression in rabbit model. Funding Acknowledgement Type of funding source: None

Vitamin D Treatment in Patients with Hashimoto’s Thyroiditis may Decrease the Development of Hypothyroidism

2016 ◽  
Vol 86 (1-2) ◽  
pp. 9-17 ◽  
Author(s):  
Bekir Ucan ◽  
Mustafa Sahin ◽  
Muyesser Sayki Arslan ◽  
Nujen Colak Bozkurt ◽  
Muhammed Kizilgul ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Hashimoto’S Thyroiditis ◽  
Healthy Control Group ◽  
Hashimoto's Thyroiditis ◽  
Control Group ◽  
Endocrine Society ◽  
Thyroid Autoantibody ◽  
Healthy Control ◽  
The Mean

Abstract.The relationship between Hashimoto’s thyroiditis and vitamin D has been demonstrated in several studies. The aim of the present study was to evaluate vitamin D concentrations in patients with Hashimoto’s thyroiditis, the effect of vitamin D therapy on the course of disease, and to determine changes in thyroid autoantibody status and cardiovascular risk after vitamin D therapy. We included 75 patients with Hashimoto’s thyroiditis and 43 healthy individuals. Vitamin D deficiency is defined as a 25-hydroxy vitamin D (25(OH)D3) concentration less than 20ng/mL. Vitamin D deficient patients were given 50.000 units of 25(OH)D3 weekly for eight weeks in accordance with the Endocrine Society guidelines. All evaluations were repeated after 2 months of treatment. Patients with Hashimoto’s thyroiditis had significantly lower vitamin D concentrations compared with the controls (9.37±0.69 ng/mL vs 11.95±1.01 ng/mL, p < 0.05, respectively). Thyroid autoantibodies were significantly decreased by vitamin D replacement treatment in patients with euthyroid Hashimoto’s thyroiditis. Also, HDL cholesterol concentrations improved in the euthyroid Hashimoto group after treatment. The mean free thyroxine (fT4) concentrations were 0.89±0.02 ng/dL in patients with Hashimoto’s thyroiditis and 1.07±0.03 ng/dL in the healthy control group (p < 0.001). The mean thyroid volumes were 7.71±0.44 mL in patients with Hashimoto’s thyroiditis and 5.46±0.63 mL in the healthy control group (p < 0.01). Vitamin D deficiency is frequent in Hashimoto’s thyroiditis and treatment of patients with this condition with Vitamin D may slow down the course of development of hypothyroidism and also decrease cardiovascular risks in these patients. Vitamin D measurement and replacement may be critical in these patients.

Mental Health Among Adolescents from Returned Portuguese Immigrant Families

2010 ◽  
Vol 69 (3) ◽  
pp. 131-139 ◽  
Author(s):  
Félix Neto
Keyword(s):  
Mental Health ◽  
Health Outcomes ◽  
Mental Health Problems ◽  
Immigrant Families ◽  
Health Problems ◽  
Mental Health Outcomes ◽  
Control Group ◽  
Older Adolescents ◽  
The Mean ◽  
Portuguese Adolescents

This study investigated mental health problems and their predictors among adolescents from returned immigrant families. The sample consisted of 360 returned adolescents (mean age = 16.8 years; SD = 1.9). The mean duration of a sojourn in Portugal for the sample was 8.2 years (SD = 4.5). A control group of 217 Portuguese youths were also included in the study. Adolescents from immigrant families reported mental health levels similar to those of Portuguese adolescents who have never migrated. Girls showed more mental health problems than boys. Younger adolescents showed fewer mental health problems than older adolescents. Adaptation variables contributed to mental health outcomes even after acculturation variables were accounted for. Implications of the study for counselors are discussed.

Sign in / Sign up

Export Citation Format

Share Document