scholarly journals Effect of long-term consumption of oxidized polyunsaturated fatty acids on atherosclerotic lesion in LDL receptor knockout mice

2020 ◽  
Author(s):  
Marina Sayuri Nogueira
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Knockout Mice ◽  
Ldl Receptor ◽  
Atherosclerotic Lesion ◽  
Ldl Receptor Knockout Mice

Heme Oxygenase-1 Inhibits Atherosclerotic Lesion Formation in LDL-Receptor Knockout Mice

2001 ◽  
Vol 88 (5) ◽  
pp. 506-512 ◽  
Author(s):  
Kazunobu Ishikawa ◽  
Daisuke Sugawara ◽  
Xu-ping Wang ◽  
Kazunori Suzuki ◽  
Hiroyuki Itabe ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Knockout Mice ◽  
Ldl Receptor ◽  
Atherosclerotic Lesion ◽  
Heme Oxygenase 1 ◽  
Lesion Formation ◽  
Ldl Receptor Knockout Mice ◽  
Atherosclerotic Lesion Formation

scholarly journals Hematopoietic upstream stimulating factor 1 deficiency is associated with increased atherosclerosis susceptibility in LDL receptor knockout mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Menno Hoekstra ◽  
Baoyan Ren ◽  
Pirkka-Pekka Laurila ◽  
Reeni B. Hildebrand ◽  
Jarkko Soronen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Knockout Mice ◽  
Ldl Receptor ◽  
Atherosclerotic Lesion ◽  
Functional Expression ◽  
Upstream Stimulatory Factor ◽  
Wild Type Littermate ◽  
Brown Adipose ◽  
Total Body ◽  
Ldl Receptor Knockout Mice

AbstractTotal body upstream stimulatory factor 1 (USF1) deficiency in mice is associated with brown adipose tissue activation and a marked protection against the development of obesity and atherosclerotic lesions. Functional expression of USF1 has also been detected in monocytes and monocyte-derived macrophages. In the current study we therefore tested whether selective hematopoietic USF1 deficiency can also beneficially impact the development of atherosclerosis. For this purpose, LDL receptor knockout mice were transplanted with bone marrow from USF1 knockout mice or their wild-type littermate controls and subsequently fed a Western-type diet for 20 weeks to stimulate atherosclerotic lesion development. Strikingly, absence of USF1 function in bone marrow-derived cells was associated with exacerbated blood leukocyte (+ 100%; P < 0.01) and peritoneal leukocyte (+ 50%; P < 0.05) lipid loading and an increased atherosclerosis susceptibility (+ 31%; P < 0.05). These effects could be attributed to aggravated hyperlipidemia, i.e. higher plasma free cholesterol (+ 33%; P < 0.001) and cholesteryl esters (+ 39%; P < 0.001), and the development of hepatosteatosis. In conclusion, we have shown that hematopoietic USF1 deficiency is associated with an increased atherosclerosis susceptibility in LDL receptor knockout mice. These findings argue against a contribution of macrophage-specific USF1 deficiency to the previously described beneficial effect of total body USF1 deficiency on atherosclerosis susceptibility in mice.

scholarly journals Dietary Fruit and Vegetable Supplementation Suppresses Diet-induced Atherosclerosis in LDL Receptor Knockout Mice (OR24-07-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Weimin Guo ◽  
Sharon Kim ◽  
Dayong Wu ◽  
Lijun Li ◽  
Michael Thomas ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Knockout Mice ◽  
Agricultural Research ◽  
Ldl Receptor ◽  
Atherosclerotic Lesion ◽  
Plasma Lipid ◽  
Aortic Lesion ◽  
Freeze Dried ◽  
Underlying Mechanisms ◽  
Ldl Receptor Knockout Mice

Abstract Objectives Epidemiological studies have shown that consumption of fruits and vegetables (F&V) is inversely associated with incidence of cardiovascular disease (CVD). However, the evidence for causality and underlying mechanisms is lacking. Our objective was to determine if increased consumption of F&V could prevent atherosclerosis and its underlying mechanisms. Methods A unique blend of the most commonly consumed 24 F&V was freeze-dried into a powder and mixed into diets. Thirty six 4-week old male LDL receptor knockout mice were randomly assigned to one of 3 diet groups (12/group): low fat (LF, 10 kcal% fat), high-fat (27 kcal% fat) with 0% F&V (HF), and HF plus 15% F&V diet (HF + FV, equivalent to 8–9 servings for humans). After 20 weeks, mice were euthanized and blood, aorta, and liver tissue were collected. Aortic atherosclerotic lesion, hepatic steatosis, plasma lipid profile and pro-inflammatory cytokine levels were measured. Results No significant differences were found in body weight among the 3 groups. Mice fed HF diet had larger aortic atherosclerotic lesion and hepatic steatosis area than mice fed LF diet by 6.5 and 1.9 fold, respectively (p < 0.001). HF + FV group had 80% less aortic lesion and hepatic steatosis than HF group (p < 0.001). Mice fed HF diet had significantly higher plasma TG and LDL and lower HDL levels than mice fed LF diet, and this dyslipidemia was prevented by F&V supplementation. Further, HF + FV group had lower plasma TNFα levels compared to HF0 group (p < 0.05). Spearman correlation analysis showed that aortic atherosclerotic lesion and hepatic steatosis area were negatively correlated with plasma HDL (p < 0.001) and significantly and positively correlated with TNFα, and the ratios of LDL/HDL, TG/HDL, and non HDL/HDL. Conclusions Our results demonstrate a causal role of high intake of F&V in preventing HF-induced atherosclerosis and hepatic steatosis, which may be mediated through improved dyslipidemia and reduced inflammation. Funding Sources U.S. Department of Agriculture – Agricultural Research Service. Supporting Tables, Images and/or Graphs

scholarly journals Upregulation of hepatic LDL transport by n-3 fatty acids in LDL receptor knockout mice

2002 ◽  
Vol 43 (5) ◽  
pp. 772-784 ◽  
Author(s):  
Chandna Vasandani ◽  
Abdallah I. Kafrouni ◽  
Antonella Caronna ◽  
Yuriy Bashmakov ◽  
Michael Gotthardt ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Knockout Mice ◽  
Ldl Receptor ◽  
Ldl Transport ◽  
Ldl Receptor Knockout Mice

IL-8 receptor antagonist SB 265610 reduces atherosclerotic lesion areas in LDL receptor knockout mice

2000 ◽  
Vol 151 (1) ◽  
pp. 196-197 ◽  
Author(s):  
G.M. Benson ◽  
D. Grimsditch ◽  
M. Vidgeon-Hart ◽  
P. Groot ◽  
K. Suckling ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Knockout Mice ◽  
Ldl Receptor ◽  
Atherosclerotic Lesion ◽  
Ldl Receptor Knockout Mice
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