New insights of low-temperature plasma effects on seeds germination of Platycodon grandiflorum

2021 ◽  
pp. 1-13
Author(s):  
Panpan Wang ◽  
Fuyuan Chen ◽  
Yulan Huang ◽  
Hunan Sun ◽  
Min Fan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Germination Rate ◽  
Gene Expression Profiles ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
Platycodon Grandiflorum ◽  
Beneficial Effects

Abstract Low temperature plasma (LTP) exerted beneficial effects on seed germination and crop growth. With application of LTP on seed germination of Platycodon grandiflorum, germination indexes and physiological parameters and transcriptome profile were investigated in this experiment. The results showed the seed germination rate and germinating potential of Platycodon grandiflorum in LTP increased by 24.7% and 30.7% comparing to CK respectively (p < 0.05). The SOD and CAT activity of the seed sprouts increased by 26.78% and 12.41% comparing to CK. Transcriptome results showed that 14342 up-regulated and 4366 down-regulated different expressing genes (DEGs) at LTP and CK. A considerable number of DEGs related to the plant hormone signal transduction, peroxisome, oxidative phosphorylation, starch and surose in response to low temperature plasma were identified in this study. The transcriptomic gene expression profiles present a valuable genomic tool to the molecular mechanisms of Platycodon grandiflorum seed germination underlying low temperature plasma. which can provide theoretical basis and practical guidance for cultivation and planting of Platycodon grandiflorum.

scholarly journals Transcriptomic Modification in the Cerebral Cortex following Noninvasive Brain Stimulation: RNA-Sequencing Approach

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Ben Holmes ◽  
Seung Ho Jung ◽  
Jing Lu ◽  
Jessica A. Wagner ◽  
Liudmilla Rubbi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cerebral Cortex ◽  
Rna Sequencing ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Current Intensity ◽  
Beneficial Effects ◽  
Group A ◽  
The Impact

Transcranial direct current stimulation (tDCS) has been shown to modulate neuroplasticity. Beneficial effects are observed in patients with psychiatric disorders and enhancement of brain performance in healthy individuals has been observed following tDCS. However, few studies have attempted to elucidate the underlying molecular mechanisms of tDCS in the brain. This study was conducted to assess the impact of tDCS on gene expression within the rat cerebral cortex. Anodal tDCS was applied at 3 different intensities followed by RNA-sequencing and analysis. In each current intensity, approximately 1,000 genes demonstrated statistically significant differences compared to the sham group. A variety of functional pathways, biological processes, and molecular categories were found to be modified by tDCS. The impact of tDCS on gene expression was dependent on current intensity. Results show that inflammatory pathways, antidepressant-related pathways (GTP signaling, calcium ion binding, and transmembrane/signal peptide pathways), and receptor signaling pathways (serotonergic, adrenergic, GABAergic, dopaminergic, and glutamate) were most affected. Of the gene expression profiles induced by tDCS, some changes were observed across multiple current intensities while other changes were unique to a single stimulation intensity. This study demonstrates that tDCS can modify the expression profile of various genes in the cerebral cortex and that these tDCS-induced alterations are dependent on the current intensity applied.

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