Integrative taxonomy of Mexican cycads: biogeography, morphology and DNA barcoding corroborate a new sympatric species in Ceratozamia (Zamiaceae)

Phytotaxa ◽  
2016 ◽  
Vol 268 (1) ◽  
pp. 25 ◽  
Author(s):  
LILÍ MARTÍNEZ-DOMÍNGUEZ ◽  
FERNANDO NICOLALDE-MOREJÓN ◽  
FRANCISCO VERGARA-SILVA ◽  
DENNIS W. STEVENSON
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Integrative Taxonomy ◽  
Los Tuxtlas ◽  
A New Species

A new species, from the montane region of Los Tuxtlas (state of Veracruz, Mexico), Ceratozamia subroseophylla, is proposed in accordance with the integrative taxonomy framework and through application of the taxonomic circle rationale. Our identification of this new species is the result of a thorough re-evaluation of four formerly described Ceratozamia species, namely: (1) C. miqueliana, with which it overlaps geographically; (2) C. mexicana; (3) C. robusta, which has also been previously seen as morphologically similar to C. miqueliana; and (4) C. brevifrons, which was at the center of a controversy concerning the morphological and geographic limits when compared to C. mexicana. Even though there are morphometric affinities of C. subroseophylla with this set of species, there are apomorphies in qualitative morphological characters and diagnostic nucleotidic variants established through character-based DNA barcoding to allow the recognition of this new species. The implications that our integrative taxonomy-based assessment of systematic diversity in Ceratozamia species from Veracruz have for evaluating the overall taxonomic status of the genus are discussed.

scholarly journals Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2)

2020 ◽  
Author(s):  
Claudia Isabel Navarro-Rodríguez ◽  
Alejandro Valdez-Mondragón
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Species Delimitation ◽  
Sister Group ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Taxonomic Approach ◽  
Morphological Differences ◽  
Unique Species ◽  
A New Species

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.

scholarly journals A small world: Uncovering hidden diversity in Frullania – a new species from Aotearoa-New Zealand

2013 ◽  
Vol 58 (2) ◽  
pp. 437-447 ◽  
Author(s):  
Matt von Konrat ◽  
Peter de Lange ◽  
Juan Larraín ◽  
Jörn Hentschel ◽  
Benjamin Carter ◽  
...  
Keyword(s):  
New Species ◽  
New Zealand ◽  
Taxonomic Status ◽  
Small World ◽  
Morphological Characters ◽  
Population Sampling ◽  
Aotearoa New Zealand ◽  
Species Complexes ◽  
Prior Investigation ◽  
A New Species

Abstract Frullania is a large and taxonomically complex genus. Here a new Frullania, F. toropuku von Konrat, de Lange & Larraín, sp. nov. is described from New Zealand. Frullania toropuku is placed in F. subg. Microfrullania. The new species is readily recognised by a combination of morphological characters associated with branching, the perianth, sexuality, and sporophyte, which distinguish it from all other New Zealand and regional species of Frullania. However, morphologically F. toropuku most closely resembles the widespread F. rostrata, which might well be regarded as a Southern Hemisphere equivalent of the Holarctic F. tamarisci species-complex in terms of its cryptic diversity. A combination of morphological characters associated with branching, the perianth, sexuality, and sporophyte distinguish F. toropuku from all other New Zealand and regional species of Frullania. A comparison is made between F. toropuku and morphologically allied species of botanical regions outside the New Zealand region and an artificial key is provided. In a prior investigation, maximum parsimony and maximum likelihood analyses of nuclear ribosomal ITS2 and plastidic trnL-trnF sequences from purported related species confirms its independent taxonomic status and corroborates its placement within F. subg. Microfrullania. The ongoing studies of Frullania species-complexes reveal the urgent need for more species-level phylogenies with extensive population sampling to approximate the actual diversity of Frullania, and to elucidate speciation processes and distribution range formation.

scholarly journals A new species of the genus Centruroides Marx (Scorpiones, Buthidae) from western Michoacán State, México using molecular and morphological evidence

ZooKeys ◽  
2019 ◽  
Vol 859 ◽  
pp. 31-48
Author(s):  
Ana F. Quijano-Ravell ◽  
Luis F. de Armas ◽  
Oscar F. Francke ◽  
Javier Ponce-Saavedra
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Valid Species ◽  
Morphological Evidence ◽  
Molecular Evidence ◽  
Mountain Range ◽  
A New Species ◽  
Gene Mrna

A new species of scorpion belonging to the genus Centruroides Marx, 1890 is described from the Coalcomán mountain range, western Michoacán State, Mexico. Its general aspect resembles Centruroidesruana Quijano-Ravell & Ponce-Saavedra, 2016, and C.infamatus (C. L. Koch, 1844), but it is a smaller species having lower pectinal tooth counts; also, males of C.ruana have the pedipalp chelae slightly thicker, whereas C.infamatus has a subaculear tubercle nearer to the base of the aculeus. Another species with similar aspect is Centruroidesornatus Pocock, 1902; however, a preliminary molecular analysis of the mitochondrial gene mRNA 16S showed genetic divergence (measured as p-distance) near to 10% between these species, and lower differences between the new species with respect to C.infamatus (4.63%) and C.ruana (5.07%). The molecular evidence together with the morphological characters (integrative taxonomy) are sufficient for recognizing the Coalcomán population as a separate and valid species.

Effectiveness of DNA barcoding markers in the description of a new and unusual calyptrate species of Myrcianthes (Myrtaceae)

Phytotaxa ◽  
2016 ◽  
Vol 284 (3) ◽  
pp. 203
Author(s):  
CARLOS PARRA-O. ◽  
A. F. BOHÓRQUEZ-OSORIO
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Morphological Characters ◽  
Unusual Combination ◽  
A New Species

A new species, Myrcianthes roncesvallensis is described and illustrated from Andean forests of Tolima, Colombia. Due to the unusual combination of morphological characters in this new species never been found previously in a Colombian Myrtaceae, such as having a closed calyx, dichasial inflorescence and an eugenioid embryo, three DNA barcoding markers (rbcL, matK and ITS) were used to confirm the genus in which this species should be described. Taxonomic affinities of the new species within the genus are discussed.

Taxonomic notes on Papilio ocypete Fabricius, 1776 and Papilio helle Cramer, 1779 with description of two new similar species from South America (Lepidoptera: Nymphalidae: Satyrinae)

Zootaxa ◽  
2018 ◽  
Vol 4425 (1) ◽  
pp. 115 ◽  
Author(s):  
MOHAMED BENMESBAH ◽  
THAMARA ZACCA ◽  
MIRNA M CASAGRANDE ◽  
OLAF H H MIELKE ◽  
GERARDO LAMAS ◽  
...  
Keyword(s):  
New Species ◽  
South America ◽  
Dna Sequence ◽  
Male Genitalia ◽  
Taxonomic Status ◽  
Sympatric Species ◽  
Similar Species ◽  
Complex Species ◽  
Species Groups ◽  
A New Species

The discovery of a new species of satyrine butterfly, Magneuptychia louisammour Benmesbah & Zacca, sp. n., that is phenotypically similar to a sympatric species widely known as Magneuptychia ocypete (Fabricius, 1776), led to a review of the taxonomic status of M. ocypete. A neotype for Papilio ocypete Fabricius, 1776 is designated and its redescription is provided. Neonympha sabina C. Felder & R. Felder, 1867 and Euptychia helle var. olivacea Aurivillius, 1929 are treated as junior subjective synonyms of P. ocypete and a lectotype is designated for each name. A neotype for the preoccupied name Papilio helle Cramer, 1779, and its replacement name Magneuptychia fugitiva Lamas, [1997], is also designated, and a redescription of this name and discussion of its taxonomic status are provided. We describe an additional phenotypically similar species, Magneuptychia kamel Benmesbah & Zacca, sp. n. from the western Amazon and raise the name Magneuptychia opima sheba Brévignon & Benmesbah, 2012 to species rank (stat. rev.) on the basis of morphological and DNA sequence evidence. We also discuss the difficulties regarding taxonomic and geographical delimitation in these complex species groups. Observations on Euptychiina behaviour are also provided. Finally, based on external characters and male genitalia, a proposed preliminary arrangement of two species groups is proposed, including other species of Magneuptychia Forster, 1964 and Cissia Doubleday, 1848. 

scholarly journals The first molecular insight into the genus Turanium Baeckmann, 1922 (Coleoptera: Cerambycidae: Callidiini) with a description of a new species from Middle Asia

2021 ◽  
Vol 79 ◽  
pp. 465-484
Author(s):  
Lech Karpiński ◽  
Wojciech T. Szczepański ◽  
Radosław Plewa ◽  
Lech Kruszelnicki ◽  
Katarzyna Koszela ◽  
...  
Keyword(s):  
New Species ◽  
Strong Support ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Central Asian ◽  
Ecological Associations ◽  
Coi Sequences ◽  
A New Species ◽  
Insight Into ◽  
Key Characters

This paper sheds the first light on the phylogeny of the Central Asian genus Turanium Baeckmann, 1922. By applying an integrative taxonomy approach, we revealed and described a new species from Kyrgyzstan—Turanium losi Karpiński, Plewa & Hilszczański sp. nov. Distinguishing characters from closely related Turanium pilosum (Reitter, 1891) are presented and their ecological associations are discussed. The key characters, including the male terminalia, were examined by means of scanning electron microscopy. High-quality stacked photographs of the habitus of the specimens are presented for both species and their geographical distributions are mapped. While the new species shows stable morphological characters that allow its differentiation from T. pilosum and the COI genetic distance between them is approx. 3%, the different species delimitation methods gave discordant results. Although the new species remained unrecognized for so long, it seems that these cerambycids are common in the region and both can be considered potentially invasive as they are apparently highly polyphagous. It has also been documented that they occur sympatrically in Kyrgyzstan. Both the Bayesian and maximum likelihood analyses of COI sequences confirmed the monophyly of the genus Turanium with strong support (PP 1 and BS 90, respectively). Moreover, the recently revealed polyphyly of the tribe Callidiini was supported by our analyses and, consequently, the discussion on the establishment of a new tribe Ropalopini is raised. This study further corroborates the effectiveness of DNA barcoding as a tool in detecting new species and provides some of the first sequences for Central Asian cerambycids, which remain almost completely unknown in terms of molecular studies.

Description of a new species of the genus Maua Distant (Hemiptera, Cicadidae) from China

Zootaxa ◽  
2021 ◽  
Vol 4949 (3) ◽  
pp. 582-588
Author(s):  
JIALI WANG ◽  
JOANNES P. DUFFELS ◽  
CONG WEI
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Intraspecific Variation ◽  
Male Genitalia ◽  
Morphological Characters ◽  
Coi Gene ◽  
Morphological Observation ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

A new species, Maua squeala sp. nov., is described from China. This species is similar to M. affinis Distant, 1905 and M. palawanensis Duffels, 2009, but can be distinguished by the shorter and more slender body of the new species, the lateral fasciae on the mesonotum and the shape of the male genitalia. The intraspecific variation of this species is discussed based on morphological observation combined with sequences of partial mitochondrial COI gene (DNA barcoding) of individuals exhibiting different morphological characters. 

A new troglobiotic Protaphorura (Collembola, Hexapoda) from the Siberia, Russia

Zootaxa ◽  
2017 ◽  
Vol 4350 (1) ◽  
pp. 185 ◽  
Author(s):  
ANDREA PARIMUCHOVÁ ◽  
ĽUBOMÍR KOVÁČ ◽  
MARTINA ŽUROVCOVÁ ◽  
OĽGA IVANOVNA KADEBSKAYA
Keyword(s):  
New Species ◽  
Body Size ◽  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Marker Gene ◽  
Taxonomic Status ◽  
Cytochrome Oxidase I ◽  
Karst Cave ◽  
Irkutsk Region ◽  
A New Species

A new species of Protaphorura Absolon, 1901 (Collembola, Onychiuridae), P. cykini sp.nov., is described from a cold karst cave in the Irkutsk region, Siberia, Russia. It is an obligate cave species with the largest body size (4.3–5.6 mm) of all congeners. The species is further characteristic by the pseudocellar formula as 3(2)2/022/33343, high number of vesicles in postantennal organ (65–71) and subapical organite protected with two papillae. A partial sequence of cytochrome oxidase I (COI DNA barcoding marker) gene is used to verify the taxonomic status of the new species and the barcode sequence is compared with other congeners available in GenBank database. Distribution and diversity of cave Collembola of Siberia is discussed. 

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