Saprobic Dothideomycetes in Thailand: Phaeoseptum hydei sp. nov., a new terrestrial ascomycete in Phaeoseptaceae

Phytotaxa ◽  
2020 ◽  
Vol 449 (2) ◽  
pp. 149-163
Author(s):  
DHANUSHKA N. WANASINGHE ◽  
PETER E. MORTIMER ◽  
CHANOKNED SENWANNA ◽  
RATCHADAWAN CHEEWANGKOON
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
University Campus ◽  
Translation Elongation ◽  
Delonix Regia ◽  
The Family ◽  
Elongation Factor 1 Alpha ◽  
Vertical Septum ◽  
Elongation Factor 1

During a survey of saprobic microfungi in Thailand, a dothideomycetous fungus was found on a dead twig of Delonix regia, on the Chiang Mai University campus. This fungus is characterized by fully immersed ascomata under a small blackened pseudoclypeus, pseudoparenchymatous peridium, cellular pseudoparaphyses, cylindrical-clavate asci with a distinct pedicel, overlapping 3–4-seriate, pale to dark brown, broadly fusoid, 7–9-transversally septate ascospores with a vertical septum in nearly all median cells. Multigene phylogenetic analyses, using partial sequences from the 28S nrRNA gene (LSU), 18S nrRNA gene (SSU), internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon and the translation elongation factor 1-alpha region (TEF) demonstrated a monophyletic affiliation of the new strain, accommodating the species of Phaeoseptum in the family Phaeoseptaceae. With further morphological and phylogenetic investigations, we justify the new fungus as a novel species, Phaeoseptum hydei in Phaeoseptaceae. Detailed descriptions and illustrations are provided for Phaeoseptum hydei and this novel species compared with the remaining species found in the genus. An updated checklist of microfungi recorded on Delonix regia from around the world is also provided.

scholarly journals Neptunomyces aureus gen. et sp. nov. (Didymosphaeriaceae, Pleosporales) isolated from algae in Ria de Aveiro, Portugal

MycoKeys ◽  
2019 ◽  
Vol 60 ◽  
pp. 31-44 ◽  
Author(s):  
Micael F. M. Gonçalves ◽  
Tânia F. L. Vicente ◽  
Ana C. Esteves ◽  
Artur Alves
Keyword(s):  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Its Region ◽  
Physiological Data ◽  
New Taxon ◽  
Translation Elongation ◽  
Ria De Aveiro ◽  
The Family ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

A collection of fungi was isolated from macroalgae of the genera Gracilaria, Enteromorpha and Ulva in the estuary Ria de Aveiro in Portugal. These isolates were characterized through a multilocus phylogeny based on ITS region of the ribosomal DNA, beta-tubulin (tub2) and translation elongation factor 1 alpha (tef1-α) sequences, in conjunction with morphological and physiological data. These analyses showed that the isolates represented an unknown fungus for which a new genus, Neptunomycesgen. nov. and a new species, Neptunomyces aureussp. nov. are proposed. Phylogenetic analyses supported the affiliation of this new taxon to the family Didymosphaeriaceae.

scholarly journals Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera

2020 ◽  
Vol 70 (7) ◽  
pp. 4321-4328
Author(s):  
Cristina Torcato ◽  
Micael F. M. Gonçalves ◽  
Edgar Rodríguez-Gálvez ◽  
Artur Alves
Keyword(s):  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
The Other ◽  
Translation Elongation Factor ◽  
Sequence Analyses ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef-1α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus Clonostachys. Single- and multi-locus gene phylogenies confirmed the isolates as representing members of the genus Clonostachys, more closely related to species in the subgenus Bionectria. In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species Clonostachys pseudochroleuca and the other one distinct from all known species of the genus Clonostachys. These isolates are recognized as representing a novel species species for which the name Clonostachys viticola is proposed.

scholarly journals Molecular characterization and phylogenetic analyses of Lophodermella needle pathogens (Rhytismataceae) on Pinus species in the USA and Europe

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11435
Author(s):  
Jessa P. Ata ◽  
Kelly S. Burns ◽  
Suzanne Marchetti ◽  
Isabel A. Munck ◽  
Ludwig Beenken ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Phenotypic Characters ◽  
Elongation Factor 1 Alpha ◽  
The Usa ◽  
Phylogenetic Resolution ◽  
Elongation Factor 1

Increasing prevalence of conifer needle pathogens globally have prompted further studies on pathogen identification and a better understanding of phylogenetic relationships among needle pathogens. Several Lophodermella species can be aggressive pathogens causing needle cast in natural pine forests in the USA and Europe. However, their relationships with other Rhytismataceae species have historically been based on similarities of only limited phenotypic characters. Currently, no molecular studies have been completed to elucidate their relationships with other Lophodermella needle pathogens. This study collected and sequenced three gene loci, namely: internal transcribed spacer, large ribosomal subunit, and translation elongation factor 1-alpha, from five Lophodermella needle pathogens from North America (L. arcuata, L. concolor, L. montivaga) and Europe (L. conjuncta and L. sulcigena) to distinguish phylogeny within Rhytismatacaeae, including Lophophacidium dooksii. Phylogenetic analyses of the three loci revealed that all but L. conjuncta that were sampled in this study consistently clustered in a well-supported clade within Rhytismataceae. The multi-gene phylogeny also confirmed consistent nesting of L. dooksii, a needle pathogen of Pinus strobus, within the clade. Potential synapomorphic characters such as ascomata position and ascospore shape for the distinct clade were also explored. Further, a rhytismataceous species on P. flexilis that was morphologically identified as L. arcuata was found to be unique based on the sequences at the three loci. This study suggests a potential wider range of host species within the genus and the need for genetic characterization of other Lophodermella and Lophophacidium species to provide a higher phylogenetic resolution.

scholarly journals Ramichloridium endophyticum sp. nov., a novel species of endophytic fungus from Potamogeton pectinatus

2020 ◽  
Vol 70 (5) ◽  
pp. 3433-3439
Author(s):  
Hua Zheng ◽  
Zefen Yu ◽  
Jianping Xu ◽  
Rafael F. Castañeda-Ruiz ◽  
Min Qiao
Keyword(s):  
Novel Species ◽  
Elongation Factor ◽  
Internal Transcribed Spacers ◽  
Potamogeton Pectinatus ◽  
Full Description ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pr China ◽  
Alpha Gene ◽  
Elongation Factor 1

During a survey of endophytic fungi in aquatic plants collected from Tibet, PR China, a novel species, Ramichloridium endophyticum, was isolated from Potamogeton pectinatus. This novel species differs from other species of the genus Ramichloridium by its finely verrucose, obovoid, ellipsoidal–obovoid and occasionally subglobose conidia. Phylogenetic analysis of the combined sequences of the internal transcribed spacers (ITS) and the translation elongation factor 1-alpha gene (tef1-α) confirmed that the isolated strain represents a member of the genus Ramichloridium. A full description, illustrations and a phylogenetic tree showing the position of R. endophyticum are provided.

Neopestalotiopsis cavernicola sp. nov. from Gem Cave in Yunnan Province, China

Phytotaxa ◽  
2021 ◽  
Vol 512 (1) ◽  
Author(s):  
XIANGFU LIU ◽  
SAOWALUCK TIBPROMMA ◽  
FAN ZHANG ◽  
JIANCHU XU ◽  
K.W.T. CHETHANA ◽  
...  
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Full Description ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Cave Habitat ◽  
Elongation Factor 1 ◽  
A New Species

In this paper we describe a new species of cave fungus belonging to Sporocadaceae (Amphisphaeriales), collected from Gem Cave, Fumin County, Yunnan Province, China. Initial morphological observations confirmed that our fungal collection is a pestalotioid species. Phylogenetic analyses of combined internal transcribed spacer (ITS), β-tubulin (TUB) and translation elongation factor 1-alpha (TEF1α) gene sequence dataset confirmed that our fungus forms an independent branch within Neopestalotiopsis. Thus, we describe our fungus as a new species of Neopestalotiopsis based on both morphology and multigene phylogeny. This is the first-ever report of Neopestalotiopsis from a cave habitat. A full description, micrographs and a phylogenetic tree showing the placement of the new species are provided.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

scholarly journals Neopestalotiopsis Species Associated with Flower Diseases of Macadamia integrifolia in Australia

2021 ◽  
Vol 7 (9) ◽  
pp. 771
Author(s):  
Kandeeparoopan Prasannath ◽  
Roger G. Shivas ◽  
Victor J. Galea ◽  
Olufemi A. Akinsanmi
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Macadamia Integrifolia ◽  
Eastern Australia ◽  
Elongation Factor 1 Alpha ◽  
Molecular Phylogenetic ◽  
Flower Disease ◽  
Elongation Factor 1

Macadamia (Macadamia integrifolia) is native to eastern Australia and produces an edible nut that is extensively cultivated in commercial orchards in several countries. Little is known about the diversity of fungi associated with diseases of macadamia inflorescences. A survey of fungi associated with the dry flower disease of macadamia detected several isolates of Neopestalotiopsis (Pestalotiopsidaceae, Sordariomycetes). Five new species of Neopestalotiopsis were identified based on molecular phylogenetic analyses of concatenated gene sequences of the internal transcribed spacer (ITS), β-tubulin (TUB), and the translation elongation factor 1-alpha (TEF1α). The new species are named Neopestalotiopsis drenthii, N. maddoxii, N. olumideae, N. vheenae, and N. zakeelii, and are described by molecular, morphological, and cultural characteristics. The ecology of the isolates and their pathogenic, saprophytic, or commensal ability were not determined.

scholarly journals Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

MycoKeys ◽  
2021 ◽  
Vol 80 ◽  
pp. 77-90
Author(s):  
Xianhong Wang ◽  
Yashuang Guo ◽  
Yamin Du ◽  
Ziling Yang ◽  
Xinzhong Huang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Elongation Factor 1

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

Cladosporium omanense, a new endophytic species from Zygophyllum coccineum in Oman

Phytotaxa ◽  
2019 ◽  
Vol 388 (1) ◽  
pp. 145 ◽  
Author(s):  
BOSHRA A. HALO ◽  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
RASHID A. AL-YAHYAI ◽  
ABDULLAH M. AL-SADI
Keyword(s):  
Sequence Data ◽  
Novel Species ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Cladosporium Sphaerospermum ◽  
Elongation Factor 1 Alpha ◽  
Desert Habitat ◽  
Elongation Factor 1 ◽  
Partial Translation

The genus Cladosporium is cosmopolitan in distribution and can be isolated from various sources including plants as endophytes. A strain of Cladosporium was isolated from living leaves of Zygophyllum coccineum in a desert habitat in Oman. Phylogenetic analysis based on the combined internal transcribed spacer (ITS), partial translation elongation factor 1-alpha (TEF) and partial actin (ACT) sequence data shows that this is a novel species, which groups with Cladosporium sphaerospermum complex. The new species is introduced as Cladosporium omanense and is described, illustrated and compared with other known species in the genus. Cladosporium omanense differs from its phylogenetically related species C. endophyticum by its fast growing colonies, longer macronematous and micronematous conidiophores, thickened-walled conidia with conspicuously protuberant shrivelling surface ornamentations.

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