Development of the Opto-Mechanical Scanning System for Biological Imaging Applications

2014 ◽  
Vol 6 (1) ◽  
pp. 103-104
Author(s):  
Jong-In Youn
2021 ◽  
Vol 2140 (1) ◽  
pp. 012032
Author(s):  
V L Khmelev ◽  
A F Fominykh

Abstract This article observe a using of active infrared beam location as roadway surface quality control. Changes in the spatial structure of the emitted IR radiation by surfaces within the capture scene allow creating a depth map of this scene. An optical camera makes it possible to use classical computer vision methods for stitching a depth map. For testing the possibility of using this approach, we made statistical studies on a multiple sample of distance measurements. Here we explain two experimental schemes with a programmable mechanical scanning system. The first one, we had determined the distance, which the image is capture accurately. The second, we measure the planar resolution, a minimum size of the defect that recognize by the infrared beam location system.


Author(s):  
Yelizaveta Meleshko ◽  
◽  
Dmytro Bakin ◽  

The goal of this work was to create a method for sectoral rasterization for images on video devices with mechanical scanning that can be used for advertising purposes. Preparing an image for display on a machine with a mechanical sector scan requires the creation of software for transferring a classic rectangular raster to a sector raster. When implementing software for an embedded computing system of mechanical image scanning for advertising purposes, the task was to reproduce a raster image using concentrically located circles, which are divided into sectors. The ambiguity of the transition from a square to a sectorial raster lies in the variable area of the sectors as they move away from the center of the image. According to this fact, there are several sectors for each of the pixels near the central zone of the image, and several pixels for one sector to the periphery of the image. There is not possible to increase the resolution of a sector image due to hardware limitations, so a new method of sector rasterization for an image on a mechanically scanned video device was developed. The developed method combines algorithms that make it possible to transform a raster image into a sectorial raster using linear interpolation depending on the distance of the sector from the center of the image. Attention was also paid to assessing the time of the next complete revolution when starting the mechanical scanning system, which made it possible to reduce the time for obtaining a stable image from the moment the device was turned on. So, as the result of the research, the method of sectorial image rasterization was developed, as well as the algorithms that allow converting a raster image into a sectorial image were developed. Вased on the developed algorithms, the software product for an embedded image reproduction system and the software product for real-time video transmission over Wi-Fi communication with the conversion of a rectangular raster into a sector raster were created. By combining algorithms for converting a square raster to a sector one, the quality of image reproduction on a mechanically scanned video device was improved.


1975 ◽  
Author(s):  
Reginald C. Eggleton ◽  
Kenneth W. Johnston

Micromachines ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 432 ◽  
Author(s):  
Wang ◽  
Gu ◽  
Tsui

The paper presents a novel fully integrated MEMS-based non-resonating operated 2D mechanical scanning system using a 1D push-pull actuator. Details of the design, fabrication and tests performed are presented. The current design utilizes an integrated electrostatic push-pull actuator and a SU-8 rib waveguide with a large core cross section (4 μm in height and 20 μm in width) in broadband single mode operation (λ = 0.4 μm to 0.65 μm). We have successfully demonstrated a 2D scanning motion using non- resonating operation with 201Hz in vertical direction and 20 Hz in horizontal direction. This non-resonating scanner system has achieved a field of view (FOV) of 0.019 to 0.072 radians in vertical and horizontal directions, with the advantage of overcoming its frequency shift caused by fabrication uncertainties. In addition, we observed two fundamental resonances at 201 and 536 Hz in the vertical and horizontal directions with corresponding displacements of 130 and 19 μm, or 0.072 and 0.0105 radian field of view operating at a +150 V input. A gradient index (GRIN) lens is placed at the end of the waveguide to focus the diverging beam output from the waveguide and a 20 μm beam diameter is observed at the focal plane. The transmission efficiency of the waveguide is slightly low (~10%) and slight tensile residual stress can be observed at the cantilever portion of the waveguide due to inherent imperfections in the fabrication process.


Author(s):  
Steve Lindaas ◽  
Chris Jacobsen ◽  
Alex Kalinovsky ◽  
Malcolm Howells

Soft x-ray microscopy offers an approach to transmission imaging of wet, micron-thick biological objects at a resolution superior to that of optical microscopes and with less specimen preparation/manipulation than electron microscopes. Gabor holography has unique characteristics which make it particularly well suited for certain investigations: it requires no prefocussing, it is compatible with flash x-ray sources, and it is able to use the whole footprint of multimode sources. Our method serves to refine this technique in anticipation of the development of suitable flash sources (such as x-ray lasers) and to develop cryo capabilities with which to reduce specimen damage. Our primary emphasis has been on biological imaging so we use x-rays in the water window (between the Oxygen-K and Carbon-K absorption edges) with which we record holograms in vacuum or in air.The hologram is recorded on a high resolution recording medium; our work employs the photoresist poly(methylmethacrylate) (PMMA). Following resist “development” (solvent etching), a surface relief pattern is produced which an atomic force microscope is aptly suited to image.


Author(s):  
K. N. Colonna ◽  
G. Oliphant

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.


Author(s):  
C.J. Stuart ◽  
B.E. Viani ◽  
J. Walker ◽  
T.H. Levesque

Many techniques of imaging used to characterize petroleum reservoir rocks are applied to dehydrated specimens. In order to directly study behavior of fines in reservoir rock at conditions similar to those found in-situ these materials need to be characterized in a fluid saturated state.Standard light microscopy can be used on wet specimens but depth of field and focus cannot be obtained; by using the Tandem Scanning Confocal Microscope (TSM) images can be produced from thin focused layers with high contrast and resolution. Optical sectioning and extended focus images are then produced with the microscope. The TSM uses reflected light, bulk specimens, and wet samples as opposed to thin section analysis used in standard light microscopy. The TSM also has additional advantages: the high scan speed, the ability to use a variety of light sources to produce real color images, and the simple, small size scanning system. The TSM has frame rates in excess of normal TV rates with many more lines of resolution. This is accomplished by incorporating a method of parallel image scanning and detection. The parallel scanning in the TSM is accomplished by means of multiple apertures in a disk which is positioned in the intermediate image plane of the objective lens. Thousands of apertures are distributed in an annulus, so that as the disk is spun, the specimen is illuminated simultaneously by a large number of scanning beams with uniform illumination. The high frame speeds greatly simplify the task of image recording since any of the normally used devices such as photographic cameras, normal or low light TV cameras, VCR or optical disks can be used without modification. Any frame store device compatible with a standard TV camera may be used to digitize TSM images.


Author(s):  
Judith M. Brock ◽  
Max T. Otten

A knowledge of the distribution of chemical elements in a specimen is often highly useful. In materials science specimens features such as grain boundaries and precipitates generally force a certain order on mental distribution, so that a single profile away from the boundary or precipitate gives a full description of all relevant data. No such simplicity can be assumed in life science specimens, where elements can occur various combinations and in different concentrations in tissue. In the latter case a two-dimensional elemental-distribution image is required to describe the material adequately. X-ray mapping provides such of the distribution of elements.The big disadvantage of x-ray mapping hitherto has been one requirement: the transmission electron microscope must have the scanning function. In cases where the STEM functionality – to record scanning images using a variety of STEM detectors – is not used, but only x-ray mapping is intended, a significant investment must still be made in the scanning system: electronics that drive the beam, detectors for generating the scanning images, and monitors for displaying and recording the images.


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