Long-term Preservation of Cone Photoreceptors and Restoration of Cone Function by Gene Therapy in the Guanylate Cyclase-1 Knockout (GC1KO) Mouse

The retinal degeneration 11 (rd11) mouse is a new animal model with rapid photoreceptor degeneration. The long-term efficacy of gene therapy has a direct relationship with the onset of photoreceptor degeneration or apoptosis, whereas the degeneration or apoptosis patterns of photoreceptors are still unclear in rd11 mice. The distribution patterns of cone function-related L- and S-opsin were examined by immunofluorescence staining, and the apoptosis was performed by TUNEL assay in rd11 mice. The expression pattern of L-opsin or S-opsin in rd11 retina at postnatal day (P) 14 was similar to the pattern observed in wildtype retina. With increasing age, the expression of L-opsin and S-opsin, especially S-opsin, decreased significantly in rd11 mice. The degeneration of L-opsin began around the optic nerve and expanded to the periphery of the retina, from the ventral/nasal to dorsal/temporal retina, whereas the expression of S-opsin gradually decreased from the dorsal/temporal to ventral/nasal retina. Apoptotic signal appeared at P14 and was strongest at P28 of rd11 mice. The key genes associated with apoptosis confirmed those changes. These indicated that the degeneration and apoptosis of cone photoreceptors began at P14 of rd11 mice, which was a key point for gene therapy.

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229. AAV-Mediated Expression of Secreted Human Ciliary Neurotropic Factor (hCNTF) Leads to Long-Term Preservation of Cone Photoreceptors in an Autosomal Recessive Model of Retinitis Pigmentosa

Molecular Therapy ◽

10.1016/s1525-0016(16)34564-6 ◽

2013 ◽

Vol 21 ◽

pp. S88-S89

Keyword(s):

Retinitis Pigmentosa ◽

Autosomal Recessive ◽

Recessive Model ◽

Cone Photoreceptors ◽

Neurotropic Factor ◽

Long Term Preservation ◽

Autosomal Recessive Model

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Long-term preservation of retinal function in the RCS rat model of retinitis pigmentosa following lentivirus-mediated gene therapy

Gene Therapy ◽

10.1038/sj.gt.3302460 ◽

2005 ◽

Vol 12 (8) ◽

pp. 694-701 ◽

Cited By ~ 78

Author(s):

M Tschernutter ◽

F C Schlichtenbrede ◽

S Howe ◽

K S Balaggan ◽

P M Munro ◽

...

Keyword(s):

Gene Therapy ◽

Retinitis Pigmentosa ◽

Rat Model ◽

Retinal Function ◽

Rcs Rat ◽

Long Term Preservation

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Practical use of a long term preservation method in the silkworm,Bombyx mori

10.1603/ice.2016.111717 ◽

2016 ◽

Author(s):

Tsuguru Fujii

Keyword(s):

Bombyx Mori ◽

Preservation Method ◽

Long Term Preservation ◽

Silkworm Bombyx Mori

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Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽

10.25276/2227-8281-1-65-69 ◽

2020 ◽

pp. 65-69

Author(s):

T.E. Lipatkina ◽

◽

Е.V. Karlova ◽

A.V. Zolotarev ◽

◽

...

Keyword(s):

Macular Edema ◽

Primary Open Angle Glaucoma ◽

Antihypertensive Drugs ◽

Open Angle Glaucoma ◽

Vascular Wall ◽

Open Angle ◽

Retinal Edema ◽

Long Term Preservation ◽

Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

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