Peptide Nucleic Acid–Fluorescence In Situ Hybridization for Detection of Staphylococci From Endophthalmitis Isolates: A Proof-of-Concept Study

Nimesh Patel; Darlene Miller; Nidhi Relhan; Harry W. Flynn

doi:10.1167/iovs.17-21535

Peptide Nucleic Acid–Fluorescence In Situ Hybridization for Detection of Staphylococci From Endophthalmitis Isolates: A Proof-of-Concept Study

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.17-21535 ◽

2017 ◽

Vol 58 (10) ◽

pp. 4307 ◽

Cited By ~ 2

Author(s):

Nimesh Patel ◽

Darlene Miller ◽

Nidhi Relhan ◽

Harry W. Flynn

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Proof Of Concept ◽

Concept Study

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Development and application of Peptide Nucleic Acid Fluorescence in situ Hybridization for the specific detection of Listeria monocytogenes

Food Microbiology ◽

10.1016/j.fm.2018.12.009 ◽

2019 ◽

Vol 80 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Rui Rocha ◽

José M. Sousa ◽

Laura Cerqueira ◽

Maria J. Vieira ◽

Carina Almeida ◽

...

Keyword(s):

In Situ Hybridization ◽

Listeria Monocytogenes ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Specific Detection

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Peer Review #2 of "Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method (v0.1)"

10.7287/peerj.780v0.1/reviews/2 ◽

2015 ◽

Author(s):

AL Guerrero-Barrera

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Bacterial Vaginosis ◽

Fluorescence In Situ Hybridization ◽

Peer Review ◽

Peptide Nucleic Acid ◽

Hybridization Method

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Detection of Microorganisms by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid

Peptide Nucleic Acids - Methods in Molecular Biology ◽

10.1007/978-1-0716-0243-0_13 ◽

2020 ◽

pp. 217-230

Author(s):

Ricardo Oliveira ◽

Carina Almeida ◽

Nuno F. Azevedo

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid

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Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and h

Experimental Dermatology ◽

10.1111/exd.13338 ◽

2017 ◽

Vol 26 (10) ◽

pp. 943-945 ◽

Cited By ~ 1

Author(s):

Hans Christian Ring ◽

Peter Theut Riis ◽

Lene Bay ◽

Klaus Kallenbach ◽

Thomas Bjarnsholt ◽

...

Keyword(s):

In Situ Hybridization ◽

Comparative Analysis ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Bacterial Aggregates

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Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Lactobacillus spp. in milk samples

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2012.09.024 ◽

2013 ◽

Vol 162 (1) ◽

pp. 64-70 ◽

Cited By ~ 19

Author(s):

Antonio Machado ◽

Carina Almeida ◽

Ana Carvalho ◽

Filip Boyen ◽

Freddy Haesebrouck ◽

...

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Hybridization Method ◽

Peptide Nucleic Acid Probe ◽

Milk Samples ◽

Lactobacillus Spp

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Rapid Identification of Staphylococcus aureus in Blood Cultures by a Combination of Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes and Flow Cytometry

Journal of Clinical Microbiology ◽

10.1128/jcm.43.9.4855-4857.2005 ◽

2005 ◽

Vol 43 (9) ◽

pp. 4855-4857 ◽

Cited By ~ 42

Author(s):

H. Hartmann ◽

H. Stender ◽

A. Schafer ◽

I. B. Autenrieth ◽

V. A. J. Kempf

Keyword(s):

Staphylococcus Aureus ◽

Flow Cytometry ◽

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Rapid Identification ◽

Blood Cultures ◽

Nucleic Acid Probes

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Multicenter Evaluation of a New Shortened Peptide Nucleic Acid Fluorescence In Situ Hybridization Procedure for Species Identification of Select Gram-Negative Bacilli from Blood Cultures

Journal of Clinical Microbiology ◽

10.1128/jcm.00166-10 ◽

2010 ◽

Vol 48 (6) ◽

pp. 2268-2270 ◽

Cited By ~ 21

Author(s):

M. Morgan ◽

E. Marlowe ◽

P. Della-Latta ◽

H. Salimnia ◽

S. Novak-Weekley ◽

...

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Species Identification ◽

Peptide Nucleic Acid ◽

Blood Cultures ◽

Gram Negative ◽

Hybridization Procedure

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Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria

PLoS ONE ◽

10.1371/journal.pone.0196522 ◽

2018 ◽

Vol 13 (5) ◽

pp. e0196522 ◽

Cited By ~ 9

Author(s):

Rui Rocha ◽

Carina Almeida ◽

Nuno F. Azevedo

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Pna Fish

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Optimization of a peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria and disclosure of a formamide effect

Journal of Biotechnology ◽

10.1016/j.jbiotec.2014.06.023 ◽

2014 ◽

Vol 187 ◽

pp. 16-24 ◽

Cited By ~ 19

Author(s):

Rita S. Santos ◽

Nuno Guimarães ◽

Pedro Madureira ◽

Nuno F. Azevedo

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Peptide Nucleic Acid ◽

Fish Method ◽

Pna Fish

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Assessment of Impact of Peptide Nucleic Acid Fluorescence In Situ Hybridization for Rapid Identification of Coagulase-Negative Staphylococci in the Absence of Antimicrobial Stewardship Intervention

Journal of Clinical Microbiology ◽

10.1128/jcm.02461-10 ◽

2011 ◽

Vol 49 (4) ◽

pp. 1581-1582 ◽

Cited By ~ 76

Author(s):

C. Holtzman ◽

D. Whitney ◽

T. Barlam ◽

N. S. Miller

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence In Situ Hybridization ◽

Antimicrobial Stewardship ◽

Peptide Nucleic Acid ◽

Rapid Identification ◽

Coagulase Negative Staphylococci

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