The effects of acute tryptophan depletion on affective behaviour and cognition in Brown Norway and Sprague Dawley rats

2008 ◽  
Vol 24 (4) ◽  
pp. 605-614 ◽  
Author(s):  
LAW Jans ◽  
GAH Korte-Bouws ◽  
SM Korte ◽  
A. Blokland
Keyword(s):  
Brown Norway ◽  
Acute Tryptophan Depletion ◽  
Tryptophan Depletion ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Inhalation of welding fumes reduced sperm counts and high fat diet reduced testosterone levels; differential effects in Sprague Dawley and Brown Norway rats

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Astrid Skovmand ◽  
Aaron Erdely ◽  
James M. Antonini ◽  
Timothy R. Nurkiewicz ◽  
Mohammad Shoeb ◽  
...  
Keyword(s):  
Systemic Inflammation ◽  
High Fat Diet ◽  
Serum Testosterone ◽  
Brown Norway ◽  
Welding Fumes ◽  
Sperm Production ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Norway Rats

Abstract Background Previous studies have shown that inhalation of welding fumes may induce pulmonary and systemic inflammation and organ accumulation of metal, to which spermatogenesis and endocrine function may be sensitive. Also obesity may induce low-grade systemic inflammation. This study aimed to investigate the effects on sperm production of inhaled metal nanoparticles from stainless steel welding, and the potential exacerbation by intake of a high fat diet. Both the inbred Brown Norway and the outbred Sprague Dawley rat strains were included to study the influence of strain on the detection of toxicity. Rats were fed regular or high fat (HF) diet for 24 weeks and were exposed to 20 mg/m3 of gas metal arc-stainless steel (GMA-SS) welding fumes or filtered air for 3 h/day, 4 days/week for 5 weeks, during weeks 7–12. Outcomes were assessed upon termination of exposure (week 12) and after recovery (week 24). Results At week 12, the GMA-SS exposure induced pulmonary inflammation in both strains, without consistent changes in markers of systemic inflammation (CRP, MCP-1, IL-6 and TNFα). GMA-SS exposure lowered daily sperm production compared to air controls in Sprague Dawley rats, but only in GMA-SS Brown Norway rats also fed the HF diet. Overall, HF diet rats had lower serum testosterone levels compared to rats on regular diet. Metal content in the testes was assessed in a limited number of samples in Brown Norway rats, but no increase was obsedrved. At week 24, bronchoalveolar lavage cell counts had returned to background levels for GMA-SS exposed Sprague Dawley rats but remained elevated in Brown Norway rats. GMA-SS did not affect daily sperm production statistically significantly at this time point, but testicular weights were lowered in GMA-SS Sprague Dawley rats. Serum testosterone remained lowered in Sprague Dawley rats fed the HF diet. Conclusion Exposure to GMA-SS welding fumes lowered sperm production in two strains of rats, whereas high fat diet lowered serum testosterone. The effect on sperm counts was likely not mediated by inflammation or lowered testosterone levels. The studied reproductive outcomes seemed more prone to disruption in the Sprague Dawley compared to the Brown Norway strain.

20-HETE Contributes to Myogenic Activation of Skeletal Muscle Resistance Arteries in Brown Norway and Sprague-Dawley Rats

2001 ◽  
Vol 8 (1) ◽  
pp. 45-55
Author(s):  
JEFFERSON C. FRISBEE ◽  
RICHARD J. ROMAN ◽  
JOHN R. FALCK ◽  
U. MURALI KRISHNA ◽  
JULIAN H. LOMBARD
Keyword(s):  
Skeletal Muscle ◽  
Brown Norway ◽  
Sprague Dawley ◽  
Resistance Arteries ◽  
Sprague Dawley Rats

scholarly journals Fluoxetine augments ventilatory CO2 sensitivity in Brown Norway but not Sprague Dawley rats

2013 ◽  
Vol 186 (2) ◽  
pp. 221-228 ◽  
Author(s):  
Matthew R. Hodges ◽  
Ashley E. Echert ◽  
Madeleine M. Puissant ◽  
Gary C. Mouradian
Keyword(s):  
Brown Norway ◽  
Sprague Dawley ◽  
Co2 Sensitivity ◽  
Sprague Dawley Rats

scholarly journals Pulmonary effects of inhalation of spark-generated silver nanoparticles in Brown-Norway and Sprague–Dawley rats

2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Joanna Seiffert ◽  
Alison Buckley ◽  
Bey Leo ◽  
Nicholas G. Martin ◽  
Jie Zhu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Brown Norway ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

20-HETE Contributes to Myogenic Activation of Skeletal Muscle Resistance Arteries in Brown Norway and Sprague–Dawley Rats

2001 ◽  
Vol 8 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Jefferson C Frisbee ◽  
Richard J Roman ◽  
John R Falck ◽  
U Murali Krishna ◽  
Julian H Lombard
Keyword(s):  
Skeletal Muscle ◽  
Brown Norway ◽  
Sprague Dawley ◽  
Resistance Arteries ◽  
Sprague Dawley Rats

scholarly journals Ventilatory behavior and carotid body morphology of Brown Norway and Sprague Dawley rats

2011 ◽  
Vol 178 (2) ◽  
pp. 250-255 ◽  
Author(s):  
Lucas M. Donovan ◽  
Sam Chai ◽  
Carl B. Gillombardo ◽  
Steven N. Emancipator ◽  
Kingman P. Strohl
Keyword(s):  
Carotid Body ◽  
Brown Norway ◽  
Sprague Dawley ◽  
Body Morphology ◽  
Sprague Dawley Rats

Abstract 247: Gender-specific Angiogenesis Suppression By Btg2 May Be Mediated Through Suppressed Renin Expression

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Timothy J Stodola ◽  
Daniela Didier ◽  
Michael Flister ◽  
Jozef Lazar ◽  
Andrew Greene
Keyword(s):  
Luciferase Activity ◽  
Proximal Promoter ◽  
Brown Norway ◽  
Sprague Dawley ◽  
Hek 293 ◽  
Congenic Lines ◽  
Sprague Dawley Rats ◽  
Males And Females

Angiogenesis is the formation of new microvessels from existing vascular beds. AngII, a downstream product of renin, has been shown to be essential mediator of skeletal muscle angiogenesis in Dahl Salt Sensitive (SS) and Sprague Dawley rats, C57BL/6 mice, and human endothelial cells in vitro. Using partial chromosome introgression from the Brown Norway (BN) rat into the SS rat we have created two congenic lines with small (<300 Kbp) BN substitutions that differ by 23 Kbp. The congenic regions define an angiogenesis locus that contains one gene, Btg2 . Sanger sequencing revealed no sequence variants in exons of Btg2 between the BN and SS strains. Angiogenesis was measured using an in vivo electrical stimulation model of one hindlimb, with the contralateral leg acting as the control, in males and females of both new congenic strains. Males from Btg2 BN have angiogenesis (TA=20.0±4.5% increase in vessel density in stimulated leg relative to unstimulated leg, EDL=15.8±5.8%,) while Btg2 SS males did not have angiogenesis (TA=5.1±2.2%, EDL=4.4±2.7%). Females of both strains had angiogenesis: Btg2 BN (TA=15.8±4.3%, EDL=3.4%), Btg2 SS (TA=14.5±2.0%, EDL=14.6±2.5%). Stimulation significantly increased Btg2 expression in both males and females. Btg2 SS males had a significantly greater increase in Btg2 mRNA in the stimulated muscle relative to unstimulated than Btg2 BN males (5.0±0.9 versus 2.1± 0.3), but there was no difference in stimulated females (Btg2 BN 2.5±0.2, Btg2 SS 3.2±1.5). To test the hypothesis that Btg2 impacted renin expression, we cloned the renin proximal promoter into a vector to drive luciferase, and co-transfected HEK-293 cells with this and vector(s) expressing Btg2 or Hoxb9 and Btg2 . Normalized luciferase activity was 4.52±0.30 (arbitrary units) with an empty vector control and suppressed with the Btg2 vector to 1.45±0.07. Co-expression of Btg2 and Hoxb9 lead to a further reduction in luciferase activity to 0.40±0.04. These data suggest the elevated Btg2 expression observed in Btg2 SS strain may be acting to suppress renin expression through renin proximal promoter transcription factor Hoxb9 , leading to an inhibited angiogenic response.

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