Immunofluorescent and Morphologic Studies on Swinepox

1966 ◽  
Vol 3 (5) ◽  
pp. 556-564 ◽  
Author(s):  
N. F. Cheville
Keyword(s):  
Electron Microscopy ◽  
Infected Cell ◽  
Cell Cultures ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Skin Lesions ◽  
Histologic Examination ◽  
Virus Particles ◽  
Naturally Occurring ◽  
Virus Isolates

Five virus isolates were obtained from pox-like lesions in swine. The respective skin lesions, infected cell cultures, virus particles and experimentally infected young swine were identical to that of swinepox by histologic examination, immunofluorescence and electron microscopy. The fluorescent antibody test provided a rapid and accurate means of diagnosis of naturally occurring lesions.

Diagnosis of Ovine Paramyxovirus (Parainfluenza-type 3) Infection in Sheep Using Negative Contrast Electron Microscopy and Ultramicrotomy of Infected Cell Cultures

2008 ◽  
Vol 14 (S2) ◽  
pp. 1544-1545
Author(s):  
CE Hearne ◽  
JL Cavender ◽  
DL Arellano ◽  
DL Montgomery
Keyword(s):  
Electron Microscopy ◽  
New Mexico ◽  
Infected Cell ◽  
Cell Cultures ◽  
Negative Contrast ◽  
Type 3

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

scholarly journals Procedure for Fluorescent-Antibody Staining of Virus-Infected Cell Cultures in Plastic Plates

1976 ◽  
Vol 3 (5) ◽  
pp. 537-540
Author(s):  
Alfred R. Pursell ◽  
John R. Cole
Keyword(s):  
Infected Cell ◽  
Cell Cultures ◽  
Fluorescent Antibody ◽  
Virus Infected Cell ◽  
Antibody Staining ◽  
Fluorescent Antibody Staining

Acetone fixation and fluorescent-antibody staining of virus-infected cell cultures were performed in plastic plates. Proper addition of acetone as a fixative did not alter the plastic.

scholarly journals Mumps Virus-persistently Infected Cell Cultures Release Defective Interfering Virus Particles

1982 ◽  
Vol 63 (2) ◽  
pp. 499-503 ◽  
Author(s):  
O. G. Andzhaparidze ◽  
Yu. S. Boriskin ◽  
N. N. Bogomolova ◽  
I. D. Drynov
Keyword(s):  
Infected Cell ◽  
Cell Cultures ◽  
Mumps Virus ◽  
Persistently Infected ◽  
Virus Particles

Same-Day Diagnostic Electron Microscopy of Infected Cell Cultures by a Modified Protocol for Conventional and Microwave Processing

2008 ◽  
Vol 14 (S2) ◽  
pp. 1548-1549 ◽  
Author(s):  
MG Metcalfe ◽  
D Rollin ◽  
CD Humphrey
Keyword(s):  
Electron Microscopy ◽  
New Mexico ◽  
Infected Cell ◽  
Cell Cultures ◽  
Microwave Processing

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

scholarly journals Comparison of Immunohistochemistry, Electron Microscopy, and Direct Fluorescent Antibody Test for the Detection of Bovine Coronavirus

1998 ◽  
Vol 10 (2) ◽  
pp. 152-157 ◽  
Author(s):  
Arshud M. Dar ◽  
Sanjay Kapil ◽  
Sagar M. Goyal
Keyword(s):  
Electron Microscopy ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Ease Of Use ◽  
Enzyme Linked Immunosorbent Assay ◽  
Moderate Degree ◽  
Bovine Coronavirus ◽  
Adult Cattle ◽  
Suitable Alternative ◽  
Direct Fluorescent Antibody

Bovine coronavirus (BCV) is 1 of the major causes of calf diarrhea and has also been implicated in respiratory infections of young calves and winter dysentery of adult cattle. Currently, transmission electron microscopy (TEM), direct fluorescent antibody (DFA), and enzyme-linked immunosorbent assay (ELISA) techniques are considered standard methods for the diagnosis of BCV infection. However, these techniques are not useful if fresh tissues and intestinal contents are not available for examination. The detection of viral antigens in formalin-fixed, paraffin-embedded tissues using immunohistochemistry (IHC) is a suitable alternative. In the present study, 166 tissue specimens were tested by IHC for the presence of BCV. These tissues were from animals whose feces were positive for rotavirus and/or coronavirus by TEM. Some of these samples were also tested by DFA. Thus, TEM, DFA, and IHC were compared for the detection of BCV. There was 56% agreement among the 3 methods (overall kappa = 0.368). When IHC was compared with TEM, 78% agreement was observed (kappa = 0.475). Similarly, IHC and DFA had 64% agreement (kappa = 0.277). These kappa values indicate a moderate degree of agreement between IHC and TEM; agreement between IHC and DFA was fair. The results of this study indicate that IHC may be a suitable adjunct for the detection of BCV because of its simplicity, ease of use, and relatively close correlation with TEM results.

scholarly journals Chlamydia-induced septic polyarthritis in a dog : case report

1999 ◽  
Vol 70 (1) ◽  
Author(s):  
N. Lambrechts ◽  
J. Picard ◽  
R.C. Tustin
Keyword(s):  
Fluorescent Antibody ◽  
Systemic Disease ◽  
Antibody Test ◽  
Joint Fluid ◽  
Polymerase Chain Reaction Test ◽  
Appendicular Skeleton ◽  
Antibody Staining ◽  
Naturally Occurring ◽  
Polymerase Chain ◽  
Chain Reaction Test

A systemic disease associated with pyrexia, lymphadenopathy, and arthropathy of several joints of the appendicular skeleton in a dog is described. Chlamydia-like organisms were detected on light-microscopic examination of a smear made from joint fluid aspirated from one of the affected joints. A group-specific lipopolysaccharide antigen shared by all Chlamydia spp. was demonstrated by direct fluorescent antibody staining of joint fluid, which also proved positive for chlamydia by means of the relevant polymerase chain reaction test. An indirect fluorescent antibody test on serumwas also positive, although the complement fixation test was negative. Attempts to grow the organism from joint aspirates in the yolk sac of embryonating hens' eggs and on appropriate tissue cultures, however, failed. Chlamydia spp. are considered to have played an aetiological role in this case, making it the first substantiated case of naturally-occurring arthropathy in a dog due to chlamydiosis. The origin of the infection could not be traced.

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