Toxicity of pharmaceutical wastewater on male reproductive system of Mus musculus

2007 ◽  
Vol 23 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Dayong Zhao ◽  
Chengjun Zhu ◽  
Shilei Sun ◽  
Hongfeng Yu ◽  
Li Zhang ◽  
...  
Keyword(s):  
Germ Cell ◽  
Mus Musculus ◽  
Reproductive System ◽  
Treated Group ◽  
Male Reproductive System ◽  
Spermatogenic Cells ◽  
Pharmaceutical Wastewater ◽  
Flow Cytometric ◽  
Industrial Health ◽  
Histopathological Studies

This study reports on the toxic effects of 35-days intragastric perfusion of pharmaceutical wastewater on the male reproductive system of Mus musculus. Flow cytometric analyses and staining with fluorescein diacetate (FDA) and propidium iodide (PI) were used to assess the toxicity on spermatogenic cells. Significant depletions in the relative percentages of elongated spermatid (HC), diploid spermatogonia (2C), and S-phase cells were observed. These alterations in different germ cell populations were reflected in the various germ cell ratios. The ratios of 1C : 4C and HC : 2C showed a significant decline after pharmaceutical wastewater treatment, while the 4C : 2C and 1C : 2C ratios increased significantly. FDA and PI staining displayed reduced viability of spermatogenic cells in wastewater treated group. Statistically significant percentages of sperm abnormalities showed the genotoxic potential of this pharmaceutical wastewater. Testicular histopathological studies of treated animals revealed expansion of interstitial space and reduction in the number and size of Leydig cells. Thus, the present study has established the toxicity of pharmaceutical wastewater on the reproductive biology of male mice. Toxicology and Industrial Health 2007; 23: 47—54.

Observations on the development of the male reproductive system in Gyrodactylus gasterostei Gläser, 1974 (Monogenea, Gyrodactylidae)

Parasitology ◽  
1985 ◽  
Vol 91 (3) ◽  
pp. 519-529 ◽  
Author(s):  
P. D. Harris
Keyword(s):  
Germ Cell ◽  
Sexual Reproduction ◽  
Reproductive System ◽  
Chromosome Complement ◽  
Mature Male ◽  
Male Reproductive System ◽  
Cell Divisions ◽  
Parasitic Flatworm ◽  
First Time

SUMMARYThe development of the male reproductive system in Gyrodactylus gasterostei has been followed using parasites of known age maintained on isolated hosts. The penis develops shortly after the parasite has given birth for the first time (at an age of 24–30 h at 13°C) and the first active spermatozoa appear after 40–50 h. Spermatogenesis occurs more rapidly in G. gasterostei than in any other parasitic flatworm (including those from warm-blooded hosts) in which it has been measured, and the onset of male maturity is further hastened by a reduction in the number of pre-spermatogenic germ cell divisions. Spermatogonia have a diploid chromosome complement of 12, and spermatocytes undergo meiosis to produce haploid spermatozoa. No evidence of aneuploidy in spermatozoa was obtained. Although the development of haploid spermatozoa suggests that sexual reproduction can occur, production of embryos by isolated flukes which lack a mature male system indicates that other means of reproduction may also be employed.

Protective role of caffeic acid on lambda cyhalothrin-induced changes in sperm characteristics and testicular oxidative damage in rats

2011 ◽  
Vol 28 (7) ◽  
pp. 639-647 ◽  
Author(s):  
Fatma Ben Abdallah ◽  
Hamadi Fetoui ◽  
Nassira Zribi ◽  
Feiza Fakhfakh ◽  
Leila Keskes
Keyword(s):  
Oxidative Damage ◽  
Caffeic Acid ◽  
Reproductive System ◽  
Male Fertility ◽  
Treated Group ◽  
Protective Role ◽  
Male Reproductive System ◽  
Male Rats ◽  
Lambda Cyhalothrin

The synthetic pyrethroids are expected to cause deleterious effects on most of the organs and especially on the male reproductive system. The current study was performed to assess the adverse effect of lambda cyhalothrin (LC) on reproductive organs and fertility in male rats and to evaluate the protective role of caffeic acid phenethyl ester (CAPE) in alleviating the detrimental effect of LC on male fertility. A total of 48 male rats were divided into 4 groups (12 rats each): control group received distilled water ad libitum and 1 ml of vehicle solution given intraperitoneally (i.p.); CAPE-treated group received a single i.p. dose of CAPE (10 μmol kg−1 day−1); LC-treated group received 668 ppm of LC through drinking water; and CAPE + LC-treated group received an i.p. injection of CAPE (10 μmol kg−1 day−1) 12 h before the LC administration. The experiment was conducted for 10 consecutive weeks. LC caused a significant increase in testicular malondialdehyde, catalase, superoxide dismutase, glutathione- S-transferase activities, and sperm abnormalities and a significant reduction in testicular glutathione concentration, sperm count, sperm motility, and a live sperm percentage. Conversely, treatment with CAPE improved the reduction in the sperm characteristics, LC-induced oxidative damage of testes and the testicular histopathological alterations. Results indicate that LC exerts significant harmful effects on the male reproductive system and that CAPE reduced the deleterious effects of LC on male fertility.

scholarly journals In vivo analysis of Bisphenol A induced dose-dependent adverse effects in cauda epididymis of mice

2018 ◽  
Vol 11 (3) ◽  
pp. 209-216
Author(s):  
Sanman Samova ◽  
Hetal Doctor ◽  
Ramtej Verma
Keyword(s):  
Bisphenol A ◽  
Reproductive System ◽  
Treated Group ◽  
Mitochondrial Enzymes ◽  
Enzymatic Antioxidants ◽  
Limited Information ◽  
In Vivo Analysis ◽  
Histopathological Studies ◽  
Dose Dependent

Abstract Bisphenol A is widely used as a material for the production of epoxy resins and polycarbonate plastics. It contaminates various food stuffs by getting leached out from their container lining. Limited information is available on its effects on the male reproductive system. The aim of the present study was to evaluate the extent to which bisphenol A can affect the reproductive system by measuring biochemical and histological changes in the epididymis. Inbred Swiss strain male albino mice were orally administered 80, 120 and 240 mg/kg body weight/day of BPA for 45 days. After completion of treatment, the animals were sacrificed; cauda epididymis was isolated, weighed, used for biochemical and histopathological studies. The results revealed that BPA administered for 45 days caused significant (p<0.05) and dose-dependent reduction in epididymis weight. There was significant (p<0.05) increase in lipid peroxidation and the acid phosphatase activity. Dose dependent reduction in protein, sialic acid contents, as well as the activity of enzymatic antioxidants and mitochondrial enzymes was recorded compared to vehicle treated group. The effect was dose-dependent. Histopathological alteration was observed. This study concludes that BPA causes toxicity in epididymis of mice by generating free radicals, which may be a possible reason for reduction in sperm parameters.

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