A traditional formula, Chunggan extract, attenuates thioacetamide-induced hepatofibrosis via GSH system in rats

2010 ◽  
Vol 30 (9) ◽  
pp. 1322-1332 ◽  
Author(s):  
Kyeong-Gue Kwak ◽  
Jing-Hua Wang ◽  
Jang-Woo Shin ◽  
Dong-Soo Lee ◽  
Chang-Gue Son
Keyword(s):  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Sprague Dawley ◽  
Necrosis Factor Alpha ◽  
Antifibrotic Effect ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Factor Alpha ◽  
Per Oral ◽  
Oxide Synthase

Chunggan extract (CGX) is a hepatotherapeutic herbal formula which has been traditionally used for patients suffering from various hepatic disorders. This study aimed to elucidate antifibrotic effect and mechanisms of CGX in thioacetamide (TAA) model. Hepatic fibrosis was induced in 45 Sprague-Dawley rats by TAA (200 mg kg–1, intraperitoneally [ip]) on twice per week for 12 weeks. CGX (100 or 200 mg kg–1, per oral [po]) was administrated once a day throughout the experiment. CGX treatment ameliorated serum biomarkers. CGX administration significantly attenuated distortion of histopathologic finding, and accumulation of hydroxyproline and malondialdehyde (MDA). CGX treatment significantly decreased transforming growth factor-beta (TGF-β) concentrations and inactivated hepatic stellate cells (HSCs). CGX treatment drastically restored glutathione (GSH) system, while inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) significantly down-regulated in liver tissue. CGX showed antifibrotic effect in thioacetamide-induced chronic liver injury model. Its corresponding mechanisms may be mediated via anti-oxidative stress property sustaining GSH system and inhibition of ROS production.

scholarly journals Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock

2021 ◽  
Vol 16 (1) ◽  
pp. 1122-1129
Author(s):  
Lei Wang ◽  
Shu-li Liu ◽  
Zhi-peng Xu ◽  
Qi Song ◽  
Lei Li ◽  
...  
Keyword(s):  
Hemorrhagic Shock ◽  
Intestinal Mucosa ◽  
Sprague Dawley ◽  
Necrosis Factor Alpha ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Factor Alpha ◽  
Hematoxylin And Eosin ◽  
Underlying Mechanisms ◽  
Traumatic Hemorrhagic Shock

Abstract This study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study, reverse transcription polymerase chain reaction, western blotting, and hematoxylin and eosin methods were used. There was a significant increase in the expression of toll-like receptor 4 (TLR4) in the rats that experienced traumatic hemorrhagic shock, along with increased mRNA of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. Pretreatment with Lactobacillus-containing probiotics reduced TLR4 expression, decreased phosphorylation (Ser536) and acetylation (Lys310) of p65, and decreased TNF-α and IL-6 mRNA. The probiotics combined acetate Ringer’s group showed a less severe pathological manifestation compared to the other experimental groups. Lactobacillus-containing probiotics inhibited nuclear factor-kappa B signaling via the downregulation of TLR4, resulting in inflammatory homeostasis, which might be the mechanism whereby Lactobacillus protects the intestinal mucosa from damage caused by the traumatic hemorrhagic shock.

scholarly journals Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

2013 ◽  
Vol 28 (suppl 1) ◽  
pp. 08-12 ◽  
Author(s):  
Frances Lilian Lanhellas Gonçalves ◽  
Daniel Guimarães Bittencourt ◽  
Lício Augusto Velloso ◽  
Augusto Frederico Schmidt ◽  
Rodrigo Melo Gallindo ◽  
...  
Keyword(s):  
Sprague Dawley ◽  
Necrosis Factor Alpha ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Fetal Rats ◽  
Post Test ◽  
Factor Alpha ◽  
Ifn Γ ◽  
Maternal Administration ◽  
Necrosis Factor

PURPOSE: To evaluate the effect of corticosteroids on intestinal and liver interleukin profile in an experimental model of gastroschisis in fetal rats. METHODS: Sprague-Dawley rats at 19.5 days of gestation had its fetuses operated for the creation of gastroschisis. Two groups of fetuses were studied with and without maternal administration of dexamethasone. Each group was composed of fetuses who underwent gastroschisis (G), control fetuses without manipulation (C) and sham fetuses (S). A dosage of the following interleukins was carried out in fetal intestinal and liver tissues: IL-1, IL-6, IL-10, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ). The differences between the groups and subgroups were tested by ANOVA with Tukey post-test, with significant values of p<0.05. RESULTS: Dexamethasone led to an increase in intestinal and liver IL-6 (p<0.05) and a decrease in intestinal TNF-α (p<0.001) in fetuses with gastroschisis. CONCLUSION: Corticosteroids had an effect on the intestinal interleukin profile and a small effect on the liver interleukin profile due to immunological immaturity of the fetus, and also of fetuses with gastroschisis. The steroid action may not be exclusively anti-inflammatory, but also pro-inflammatory, varying with time of pregnancy.

scholarly journals Citocinas e proteínas de fase aguda do soro como marcadores de regressão da resposta inflamatória ao tratamento da tuberculose pulmonar

2008 ◽  
Vol 34 (11) ◽  
pp. 942-949 ◽  
Author(s):  
Eliana Peresi ◽  
Sônia Maria Usó Ruiz Silva ◽  
Sueli Aparecida Calvi ◽  
Jussara Marcondes-Machado
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Tumor Necrosis ◽  
Transforming Growth Factor ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Ifn Γ ◽  
Growth Factor Beta ◽  
Necrosis Factor

OBJETIVO: Analisar o padrão de citocinas pró- e antiinflamatórias e da resposta de fase aguda (RFA) como marcadores de resposta ao tratamento da tuberculose pulmonar. MÉTODOS: Determinação dos níveis de interferon-gama (IFN-γ), tumor necrosis factor-alpha (TNF-α, fator de necrose tumoral-alfa), interleucina-10 (IL-10) e transforming growth factor-beta (TGF-β, fator transformador de crescimento-beta), pelo método ELISA, em sobrenadante de cultura de células mononucleares do sangue periférico e monócitos, assim como dos níveis de proteínas totais, albumina, globulinas, alfa-1-glicoproteína ácida (AGA), proteína C reativa (PCR) e velocidade de hemossedimentação (VHS) em 28 doentes com tuberculose pulmonar, em três tempos: antes (T0), aos três meses (T3) e aos seis meses (T6) de tratamento, em relação aos controles saudáveis, em um único tempo. RESULTADOS: Os pacientes apresentaram valores maiores de citocinas e RFA que os controles em T0, com diminuição em T3 e diminuição (TNF-α, IL-10, TGF-β, AGA e VHS) ou normalização (IFN-γ e PCR) em T6. CONCLUSÕES: PCR, AGA e VHS são possíveis marcadores para auxiliar no diagnóstico de tuberculose pulmonar e na indicação de tratamento de indivíduos com baciloscopia negativa; PCR (T0 > T3 > T6 = referência) pode também ser marcador de resposta ao tratamento. Antes do tratamento, o perfil Th0 (IFN-γ, IL-10, TNF-α e TGF-β), indutor de e protetor contra inflamação, prevaleceu nos pacientes; em T6, prevaleceu o perfil Th2 (IL-10, TNF-α e TGF-β), protetor contra efeito nocivo pró-inflamatório do TNF-α ainda presente. O comportamento do IFN-γ (T0 > T3 > T6 = controle) sugere sua utilização como marcador de resposta ao tratamento.

Teucrium polium reversed the MCD diet-induced liver injury in rats

2010 ◽  
Vol 30 (9) ◽  
pp. 1303-1312 ◽  
Author(s):  
Rahim Amini ◽  
Razieh Yazdanparast ◽  
Safiyeh Aghazadeh ◽  
Seyed H Ghaffari
Keyword(s):  
Ethyl Acetate ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Ethyl Acetate Fraction ◽  
Necrosis Factor Alpha ◽  
Nash Group ◽  
Tnf Α ◽  
High Antioxidant Activity ◽  
Factor Alpha ◽  
Teucrium Polium

In the present study, we evaluated the ability of Teucrium polium ethyl acetate fraction, with high antioxidant activity, in the treatment of nonalcoholic steatohepatitis (NASH) in rats and its possible effect on factors involved in pathogenesis of the disease. To induce NASH, a methionine and choline deficient (MCD) diet was given to N-Mary rats for 8 weeks. After NASH development, MCD-fed rats were divided into 2 groups: NASH group that received MCD diet and NASH + T group which was fed MCD diet plus ethyl acetate fraction of T. polium orally for 3 weeks. Histopathological evaluations revealed that treatment with the extract has abated the severity of NASH among the MCD-fed rats. In addition, the fraction reduced the elevated levels of hepatic tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β) gene expression and also the elevated level of malondialdehyde (MDA). In addition, the extract increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and enhanced the level of hepatic glutathione (GSH). Moreover, the fraction treatments lowered caspase-3 level and the phosphorylated form of C-Jun N-terminal kinase (JNK) and augmented the phosphrylated level of extracellular regulated kinase1/2 (ERK1/2). These results indicate that the ethyl acetate fraction of T. poium effectively reversed NASH, mainly due to its strong antioxidant and anti-inflammatory properties.

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