scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals Essential Oil Composition and Antimicrobial Activity of Aerial Parts and Ripe Fruits of Echinophora spinosa (Apiaceae) from Italy

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800
Author(s):  
Daniele Fraternale ◽  
Salvatore Genovese ◽  
Donata Ricci
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Intestinal Microflora ◽  
Central Italy ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Aerial Parts ◽  
Eubacterium Limosum

The chemical composition and antimicrobial activity of the essential oils obtained from the flowering aerial parts and ripe fruits of Echinophora spinosa L. (Apiaceae) from central Italy were analyzed by GC/MS. The major constituents of the oil from the aerial parts were β-phellandrene (34.7%), myristicin (16.5%), δ3-carene (12.6%), α-pinene (6.7%) and α-phellandrene (6.2%), and of the oil from the ripe fruits p-cymene (50.2%), myristicin (15.3%), α-pinene (15.1%) and α-phellandrene (8.1%). The two oils showed good antimicrobial activity against Clostridium difficile, C perfringens, Enterococcus faecalis, Eubacterium limosum, Peptostreptococcus anaerobius and Candida albicans with MIC values respectively of 0.25, 0.25, 0.25, 0.25, 2.25, and 0.50%, v/v, and 0.13, 0.13, 0.13, 0.13, 2.25, 0.50%, v/v, for aerial parts and ripe fruits respectively. A less significant antimicrobial activity against bifidobacteria and lactobacilli, very important in the intestinal microflora, was also detected, with MIC values higher than 4.0%, v/v.

Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

2020 ◽  
Author(s):  
Haïfa Debbabi ◽  
Ridha El Mokni ◽  
Ibrahim Jlassi ◽  
Rajesh K Joshi ◽  
Saoussen Hammami
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

scholarly journals Chemical Composition of Nardostachys grandiflora Rhizome Oil from Nepal – A Contribution to the Chemotaxonomy and Bioactivity of Nardostachys

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Prabodh Satyal ◽  
Bhuwan K. Chhetri ◽  
Noura S. Dosoky ◽  
Ambika Poudel ◽  
William N. Setzer
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Mcf 7

The essential oil from the dried rhizome of Nardostachys grandiflora, collected from Jaljale, Nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. The rhizome essential oil of N. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol A (6.0%), 1(10)-aristolen-9-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). The chemical composition of N. grandiflora rhizome oil from Nepal is qualitatively very different than those from Indian, Chinese, and Pakistani Nardostachys essential oils. In this study we have evaluated the chemical composition and biological activities of N. grandiflora from Nepal. Additionally, 1(10)-aristolen-9-ol was isolated and the structure determined by NMR, and represents the first report of this compound from N. grandiflora. N. grandiflora rhizome oil showed in-vitro antimicrobial activity against Bacillus cereus, Escherichia coli, and Candida albicans (MIC = 156 μg/mL), as well as in-vitro cytotoxic activity on MCF-7 cells.

scholarly journals Chemical composition and antimicrobial activity of berry essential oil of Juniperus oxycedrus L. (Cupressaceae) grown wild in Republic of Macedonia

2013 ◽  
Vol 59 ◽  
pp. 41-48 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Juniperus Oxycedrus ◽  
Republic Of Macedonia

Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria was Haemophilus influenzae (MIC = 125 ml/ml). The essential oils showed moderate antimicrobial activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp., Escherichia coli and Campilobacter jejuni (MIC > 500 ml/ml) and no activity against Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexnery, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus and Proteus mirabilis.

scholarly journals Chemical composition and antimicrobial activity of the essential oil from Satureja horvatii Silic (Lamiaceae)

2008 ◽  
Vol 73 (7) ◽  
pp. 703-711 ◽  
Author(s):  
Branislava Lakusic ◽  
Mihailo Ristic ◽  
Violeta Slavkovska ◽  
Jelena Antic-Stankovic ◽  
Marina Milenkovic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Micrococcus Luteus ◽  
Antimicrobial Activities ◽  
Maximum Activity ◽  
Moderate Activity ◽  
Aerial Parts ◽  
Weak Activity

The present paper describes the chemical composition and antimicrobial activity of the essential oil of the endemic species Satureja horvatii Silic, collected in Montenegro. The essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC-MS. From the 34 compounds representing 100 % of the oil, the major compound was the phenolic monoterpene thymol (63.37 %). The oil contained smaller amounts of g-terpinene (7.49 %), carvacrol methyl ether (4.92 %), carvacrol (4.67 %), p-cymene (4.52%), a-terpinene (1.81 %), borneol (1.58 %), a-thujene (1.56 %), b-caryophyllene (1.55 %) and b-myrcene (1.44 %). The antimicrobial activity of the essential oil of S. horvatii was evaluated using the agar diffusion and broth microdilution methods. The essential oil exhibited antimicrobial activity to varying degrees against all the tested strains. The maximum activity of S. horvatii oil was observed against Gram-positive bacteria (Micrococcus luteus, Staphylococcus epidermidis, Staphylococcus aureus and Enterococcus faecalis) and against the yeast (Candida albicans). The oil exhibited moderate activity against the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae and weak activity against Pseudomonas aeruginosa. This study confirms that the essential oil of S. horvatii possesses antimicrobial activities in vitro against medically important pathogens.

scholarly journals Chemical composition and antimicrobial activity of leaves essential oil of Juniperus communis (Cupressaceae) grown in Republic of Macedonia

2013 ◽  
Vol 59 ◽  
pp. 23-32 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokik ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Salmonella Enteritidis ◽  
Shigella Flexneri ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Juniperus Communis ◽  
Acinetobacter Spp

Chemical composition and antimicrobial activity of essential oils isolated from leaves of three different samples of wild growing Juniperus communis L. (Cupressaceae) from R. Macedonia was investigated. Essential oil yield ranged from 7.3 to 9.0 ml/kg. Performing GC/ FID/MS analysis, ninety components were identified, representing 86.07-93.31% of the oil. The major components of the leaves essential oil (LEO) were α-pinene (21.37-28.68%) and sabinene (2.29-16.27%), followed by limonene, terpinen-4-ol, β-elemene, trans-(E)-caryophyllene, germacrene D and δ-cadinene. Antimicrobial screening of the LEO was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. Two bacteria, Staphylococcus aureus and Streptococcus pyogenes were sensitive to antimicrobial activity of LEO (MIC = 125 µl/ml). Additionally, LEO showed moderate antimicrobial activity against Streptococcus agalactiae, Haemophilus influnzae, Corynebacterium spp. and Campylobacter jejuni (MIC > 500 µl/ml). Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexneri, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis were completely resistant to the antimicrobial effects of this.

scholarly journals Effect of nano-iron chelate on chemical composition and antimicrobial properties of Carum copticum L. essential oil and its main Terpenes from Iran

2018 ◽  
Vol 44 (4) ◽  
pp. 537-542 ◽  
Author(s):  
V Abdossi ◽  
M Kazemi
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Bacillus Cereus ◽  
Antimicrobial Properties ◽  
Flowering Stage ◽  
Iron Chelate ◽  
Carum Copticum

The effect of exogenous application of nano-iron chelate (100 and 200 mg/l) in early flowering stage on components of essential oils (EOs) of Carum copticum was evaluated. The EO extracted by hydrodistillation from Iranian C. copticum was characterized by means of GC/MS. The nano-iron chelate application increased γ- terpinene and thymol and decreased α-pinene, β-pinene and p-cymene concentration. The oil was also subjected to antimicrobial activity. C. copticum oil was found to inhibit Bacillus cereus and Candida albicans with the lowest MIC and MBC/MFC values. Thymol and carvacrol possessed the highest antimicrobial activity among the major components. These results clearly show the antimicrobial effects of the essential oil C. copticum.

scholarly journals Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Messaoud Ramdani ◽  
RIMA HAICHOUR1 ◽  
TAKIA LOGRADA ◽  
PIERRE CHALARD ◽  
GILLES FIGUEREDO4
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pinus Halepensis ◽  
Antibacterial Activities ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Aerial Parts ◽  
Methyl Butanoate

Abstract. Haichour R, Lograda T, Ramdani M, Chalard P, Figueredo G. 2020. Chemical composition and antimicrobial activity of Pinus halepensis from Algeria. Biodiversitas 21: 4345-4360. The chemical composition of Pinus halepensis essential oils and their antibacterial activities were investigated. Plant samples were collected in the flowering stage from the East locality of Algeria. The aerial parts of P. halepensis obtained from fifteen localities were subjected to a hydro-distillation, providing pale yellow viscous oils. The obtained yield average was 0.64 ± 0.37%. The analysis of the chemical composition of essential oils was performed by using GC and GC / MS. A total of thirty-seven compounds representing 99.4 ± 0.5% of the total oils were identified in P. halepensis. The hydrocarbon compounds were found to dominate essential oils in P. halepensis. In addition, the major compounds were caryophyllene-E (29.06 ± 8.5%), myrcene (19.14 ± 6.67%), a-pinene (16.86 ± 3.35%), phenyl ethyl 3-methyl butanoate (5.67 ± 2.47%), a-humulene (4.81 ± 1.43%), terpinolene (3.94 ± 1.6%) and sabinene (3.11 ± 1.84%). Based on their chemical composition, essential oil samples were clustered in five groups by UPGMA analysis. The first group includes the Tafrent essential oil population. It is characterized by the phenyl ethyl-3-methyl butanoate – Sabinene ‒ Δ3-carene chemotype. The second group was subdivided into two clusters. The first cluster or chemotype was characterized by the myrcene ‒ α-pinene ‒ caryophyllene-E. The second chemotype was characterized by caryophyllene-E ‒ α-humulene ‒ myrcene. The essential oil of P. halepensis is an effective antimicrobial against Staphylococcus aureus; Klebsiella pneumonia; Salmonella enteric spp arizonae; Listeria innocua; Proteus mirabilis and Bacillus cereus, and no effect on the E. coli and Pseudomonas aeruginosa.

Comparative studies of the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola on Klebsiella pneumonia and Candida albicans in vitro

2021 ◽  
Vol 16 (1) ◽  
pp. 52-58
Author(s):  
Elizabeth Winful ◽  
Olanikpekun Idowu ◽  
Opeoluwa O. Fasanya ◽  
Nkechi E. Egbe
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Garcinia Kola ◽  
Zones Of Inhibition

Antimicrobial resistance has become a global health problem. Although a wide range of chemotherapeutic antimicrobials are available for treatment of microbial related infections and diseases, development of resistance to these chemotherapeutic agents is rapidly on the increase. Extracts from some plants have shown some promise in antimicrobial activity. This has led to the screening of several medicinal plants for their potential antimicrobial activity. Therefore, this study was aimed at evaluating the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola against Klebsiella pneumonia and Candida albicans in vitro. The Garcinia kola seeds extracts were obtained using 70% ethanol and distilled water respectively. Phytochemical screening of Garcinia kola revealed the presence of various potent phytochemicals such as tannins, saponins, flavonoids, alkaloids and glycosides. Both extracts of the seeds were investigated for antimicrobial activity using disc diffusion and agar well diffusion sensitivity tests. The ethanol extract produced zones of inhibition of about 7.3 mm for Candida albicans only at a concentration of 800mg/ml for the disc diffusion test. For the agar well diffusion test, the aqueous extract produced zones of inhibition of about 9.5 mm, while the ethanol extract produced zone of inhibition of 19 mm against Candida albicans at a concentration of 800 mg/ml. However, at the same concentration, the ethanol extract produced zones of inhibition of about 8.5 mm against Klebsiella pneumoniae. The findings of this study revealed that Garcinia kola was not effective in treating Klebsiella pneumonia infections but has potential in treating Candida albicans infections. Keywords: Garcinia kola, Klebsiella pneumonia, Candida albicans

scholarly journals Chemical Composition and Preliminary Antimicrobial Activity of the Hydroxylated Sesquiterpenes in the Essential Oil from Piper barbatum Kunth Leaves

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 211 ◽  
Author(s):  
Paco Noriega ◽  
José Ballesteros ◽  
Alejandra De la Cruz ◽  
Tatiana Veloz
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Forest Ecosystem ◽  
Raw Materials ◽  
The Andes ◽  
Ancestral Knowledge ◽  
Antifungal Potential

This study evaluates the antimicrobial and antifungal potential of the essential oil extracted from a species located in the Andes of Ecuador, Piper barbatum Kunth, known as “cordoncillo” or “allupa”, used by the Quichua people as an antibacterial plant for washing female genitalia in cases of infection. The most abundant molecules in the essential oil were: α- phellandrene (43.16%), limonene (7.04%); some oxygenated sesquiterpenes such as: trans-sesquisabinene hydrate (8.23%), elemol (7.21%) and others. The evaluation of antimicrobial activity showed activity in all the strains analyzed; however, those in which MIC values are considered to be very strong (less than 500 µg/mL) are: Staphylococcus aureus 264 µg/mL, Streptococcus mutans 132 µg/mL, Candida albicans 132 µg/mL and Candida tropicalis 264 µg/mL. Antimicrobial bioautography defines which molecules are responsible for the activity; thus, it was possible to establish the chromatographic regions of = 0.02 and Rf = 0.04, as those with active molecules. It was established that 4 hydroxylated sesquiterpene molecules are involved: elemol (7.21%), trans-sesquisabinene hydrate (8.23%), β–eudesmol (3.49%) and 10-epi-γ-eudesmol (1.07%); the last two being the most active. The aim of this manuscript is to analyze both the ancestral knowledge of the Quichua people of Ecuador, and the chemical-biodiversity of the Andean forest ecosystem, in order to provide new raw materials of pharmaceutical interest.

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