scholarly journals Antioxidant, Antiproliferative and Antimicrobial Activities of the Volatile Oil from the Wild Pepper Piper capense Used in Cameroon as a Culinary Spice

2013 ◽  
Vol 8 (12) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Verlaine Woguem ◽  
Filippo Maggi ◽  
Hervet P. D. Fogang ◽  
Léon A. Tapondjou ◽  
Hilaire M. Womeni ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tumor Cells ◽  
Biological Activities ◽  
Human Tumor ◽  
Breast Adenocarcinoma ◽  
Moderate Activity ◽  
Oil Composition ◽  
Human Tumor Cells ◽  
Agar Disc

Wild pepper (Piper capense L.f., Piperaceae) is a spice traditionally used in western Cameroon to make soups called ‘ Nkui’ and ‘ Nah poh’. In the present work, the essential oil hydrodistilled from fruits was analyzed by GC-FID and GC-MS, and for in vitro biological activities, namely cytotoxic, antioxidant and antimicrobial, by MTT, DPPH, ABTS and agar disc diffusion methods. The oil composition was dominated by monoterpene hydrocarbons (56.5%) responsible for the pepper odor, such as β-pinene (33.2%), sabinene (10.0%) and α-pinene (8.9%). The oil induced a concentration-dependent inhibitory effect on human tumor cells MDA-MB 231 (breast adenocarcinoma), A375 (malignant melanoma) and HCT116 (colon carcinoma), showing IC50 values of 26.3, 76.0 and 22.7 μg/ml, respectively. The oil showed total antioxidant activity with a Trolox equivalent antioxidant concentration (TEAC) value of 140 μmol/g. The essential oil of P. capense proved to be an effective scavenger of the ABTS+ radical, with an activity only about 30 times lower than that of Trolox. Moderate activity was observed against the Gram-positive species Staphylococcos aureus and Enterococcus faecalis, and the yeast Candida albicans. The notable inhibition of some human tumor cells is worthy of further investigation to discover the possible mechanisms of action responsible for the observed cytotoxic effect of this essential oil.

scholarly journals Essential oil composition and cytotoxic activity in two species of the plant genus Vismia (Hypericaceae) from the Venezuelan Andes

2020 ◽  
Vol 68 (3) ◽  
Author(s):  
Janne Rojas Vera ◽  
Alexis Buitrago Díaz ◽  
Francisco A. Arvelo ◽  
Felipe J. Sojo ◽  
Alírica I. Suarez ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Tumor Cells ◽  
Biological Activities ◽  
Human Tumor ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Mcf 7

Abstract. Introduction: The Vismia genus belongs to the Hypericaceae family and comprises around 57 species of which 17 have been located in Venezuela. Previous investigations have been carried out in extracts as well as pure isolated compounds, revealing antimicrobial, antioxidant and anti-HIV, among other, biological activities. Objective: This investigation aims to determine the cytotoxic activity of essential oils from leaves of Vismia baccifera Triana & Planch (VBJ and VBV) and Vismia macrophylla Kunth (VM) collected in three different locations of the Venezuelan Andean region. Methods: Essential oils obtained by hydrodistillation were analyzed using gas chromatography-mass spectrometry (GC-MS) and their cytotoxic activity was analyzed following the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Human tumor cell lines from SKBr3, MCF-7 and PANC-1, two breast carcinomas and one pancreatic adenocarcinoma of ductal type, were tested with the oil samples and human dermis fibroblasts were used as non-tumor cells. Results: β-caryophyllene and trans-caryophyllene were present as major components in VBJ and VBV, respectively, while γ-bisabolene was the main component in the VM sample. Anticancer activity was observed on V. baccifera essential oil against SKBr3, MCF-7 and PANC-1. The selectivity index showed that VBV is highly selective against the SKBr3 cell line and has no activity against non-tumor cells. Conclusions: These results are considered a contribution to natural products research and may provide supportive data for future studies on cancer.

scholarly journals MLN8054, an Inhibitor of Aurora A Kinase, Induces Senescence in Human Tumor Cells Both In vitro and In vivo

2010 ◽  
Vol 8 (3) ◽  
pp. 373-384 ◽  
Author(s):  
Jessica J. Huck ◽  
Mengkun Zhang ◽  
Alice McDonald ◽  
Doug Bowman ◽  
Kara M. Hoar ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Aurora A Kinase ◽  
Aurora A ◽  
Human Tumor Cells

scholarly journals Radiosensitization by the Combination of SR-2508 and Paclitaxel in Hypoxic Human Tumor Cells in vitro

2007 ◽  
Vol 48 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Cheng JIN ◽  
Ling BAI ◽  
Guozhen GUO
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells

Effects of Lonidamine on Murine and Human Tumor Cells in vitro

Oncology ◽  
1984 ◽  
Vol 41 (1) ◽  
pp. 15-29 ◽  
Author(s):  
C.D. De Martinet ◽  
T. Battelli ◽  
M.G. Paggi ◽  
A. Nista ◽  
M.L. Marcante ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells

scholarly journals Antiproliferative activity of essential oils from three plants of the Brazilian Cerrado: Campomanesia adamantium (Myrtaceae), Protium ovatum (Burseraceae) and Cardiopetalum calophyllum (Annonaceae)

2020 ◽  
Vol 80 (2) ◽  
pp. 290-294 ◽  
Author(s):  
C. C. F. Alves ◽  
J. D. Oliveira ◽  
E. B. B. Estevam ◽  
M. N. Xavier ◽  
H. D. Nicolella ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cell Line ◽  
Tumor Cells ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Human Tumor ◽  
Lung Fibroblasts ◽  
Breast Adenocarcinoma ◽  
Brazilian Cerrado

Abstract Essential oils, which may be extracted from several parts of plants, have different biological activities. The Brazilian Cerrado has a large variety of plants that yield essential oils, even though many have not been studied yet. Taking into account the biodiversity of this biome, this study aimed at evaluating the antiproliferative activity of essential oils extracted from three species of plants of the Cerrado in Goiás state: Campomanesia adamantium (Cambess.) O. Berg, Protium ovatum (Engl. in Mart.) and Cardiopetalum calophyllum (Schltdl.). Essential oils were extracted from both C. adamantium and C. calophyllum leaves and from P. ovatum leaves and green fruits by hydrodistillation carried out by a Clevenger-type apparatus. The chemical composition of the essential oils was determined by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The following major chemical constituents were identified in the essential oils under investigation: β-myrcene (62.00%), spathulenol (28.78%), germacrene-B (18.27%), β-caryophyllene oxide (16.40%), β-caryophyllene (14.00%), α-pinene (11.30%), viridiflorol (9.99%), limonene (7.30%) and (Z,E)-pharnesol (6.51%). The antiproliferative activity was evaluated in different human tumor cell lines: breast adenocarcinoma (MCF-7), cervical adenocarcinoma (HeLa) and glioblastoma (M059J). A normal human cell line was included (GM07492A, lung fibroblasts). Results showed that essential oils from C. adamantium leaves got the lowest values of IC50 in all strains of tumor cells under evaluation. They were significantly lower than the ones of the normal cell line, an evidence of selectivity. It is worth mentioning that this is the first report of the antiproliferative activity of essential oils from C. adamantium , P. ovatum and C. calophyllum against human tumor cells.

scholarly journals Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo

2008 ◽  
Vol 99 (4) ◽  
pp. 810-815 ◽  
Author(s):  
Dong Yu ◽  
Emiko Sekine ◽  
Akira Fujimori ◽  
Takahiro Ochiya ◽  
Ryuichi Okayasu
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Down Regulation

scholarly journals In vitro analysis of transport and metabolism of 4′-thiothymidine in human tumor cells

2015 ◽  
Vol 42 (5) ◽  
pp. 470-474 ◽  
Author(s):  
David A. Plotnik ◽  
Stephen Wu ◽  
Geoffrey R. Linn ◽  
Franco Chi Tat Yip ◽  
Natacha Lou Comandante ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Oligomannurarate sulfate inhibits CXCL12/SDF-1-mediated proliferation and invasion of human tumor cells in vitro

2013 ◽  
Vol 34 (12) ◽  
pp. 1554-1559 ◽  
Author(s):  
Wei-wei Wen ◽  
Shao Xie ◽  
Xian-liang Xin ◽  
Mei-yu Geng ◽  
Jian Ding ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Proliferation And Invasion

scholarly journals In vitro colony formation by cryopreserved primary human tumor cells

Cryobiology ◽  
1984 ◽  
Vol 21 (2) ◽  
pp. 240-245
Author(s):  
E.D. Allen ◽  
T.C. Gau ◽  
R.B. Natale
Keyword(s):  
Tumor Cells ◽  
Colony Formation ◽  
Human Tumor ◽  
Human Tumor Cells
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