scholarly journals An interlaboratory in vitro aerosol exposure system reference study

2021 ◽  
Vol 5 ◽  
pp. 239784732199275
Author(s):  
David Thorne ◽  
Jason Adamson ◽  
Edgar Trelles Sticken ◽  
Roman Wieczorek ◽  
Holger Behrsing ◽  
...  
Keyword(s):  
Interlaboratory Comparison ◽  
In Vitro Toxicology ◽  
Exposure System ◽  
Multiple Systems ◽  
Aerosol Generation ◽  
Aerosol Exposure ◽  
In Vitro Exposure ◽  
The Usa ◽  
The Uk

Given the complexity of inhaled substances, the aerosol exposure environment has seen diversification and development of setups in conjunction with the evolving in vitro toxicology space. Each laboratory uses its in vitro exposure system differently (different protocols, adaptations, and biological analysis). Unfortunately, as systems diversify, so does the complexity of comparing multiple systems in a “standardized” manner. As yet, no one has compared simply whether these diverse systems can all generate a consistent aerosol stream, which is paramount prior to transit and exposure. This study has compared, at source, aerosol generation (using nicotine as an exposure marker) in nine in vitro whole-aerosol exposure setups (seven different systems) across five distinct geographically independent locations, including the UK, the USA, Switzerland, Germany, and Japan. The results demonstrate that, despite system-wide differences (adaptations, nuances, and application), these systems—when appropriately maintained and used under a prescribed set of established conditions can all generate a consistent and statistically comparable aerosol stream. These data will be invaluable for new researchers and established laboratories, so they may benchmark against this study. Finally, this interlaboratory comparison combined with the wealth of transit and exposure interface data, may help the environment move towards a truly validated and consistent approach to aerosol exposure. Such an approach could be replicated for other aerosolized products, such as e-cigarettes and heated tobacco products.

scholarly journals A survey of aerosol exposure systems relative to the analysis of cytotoxicity: A Cooperation Centre for Scientific Research Relative to Tobacco (CORESTA) perspective

2021 ◽  
Vol 5 ◽  
pp. 239784732110222
Author(s):  
David Thorne ◽  
Roman Wieczorek ◽  
Toshiro Fukushima ◽  
Han-Jae Shin ◽  
Robert Leverette ◽  
...  
Keyword(s):  
Scientific Research ◽  
Toxicity Testing ◽  
User Preferences ◽  
In Vitro Toxicity ◽  
Aerosol Generation ◽  
Aerosol Exposure ◽  
Comprehensive Survey ◽  
Survey Results ◽  
System Data

During a Cooperation Centre for Scientific Research Relative to Tobacco (CORESTA) meeting, the in vitro toxicity testing Sub-Group (IVT SG) met to discuss the evolving field of aerosol exposure research. Given the diversity of exposure parameters and biological endpoints being used, it was considered a high priority to investigate and contextualise the responses obtained. This is particularly driven by the inability to compare between studies on different exposure systems due to user preferences and protocol differences. Twelve global tobacco and contract research companies met to discuss this topic and formulate an aligned approach on how this diverging field of research could be appropriately compared. Something that is becoming increasingly important, especially in the light of more focused regulatory scrutiny. A detailed and comprehensive survey was conducted on over 40 parameters ranging from aerosol generation, dilution and data analysis across eight geographically independent laboratories. The survey results emphasise the diversity of in vitro exposure parameters and methodologies employed across the IVT SG and highlighted pockets of harmonisation. For example, many of the biological protocol parameters are consistent across the Sub-Group. However, variables such as cell type and exposure time remain largely inconsistent. The next steps for this work will be to map parameters and system data against biological findings and investigate whether the observed inconsistencies translate into increased biological variability. The results from the survey provide improved awareness of parameters and nuances, that may be of substantial benefit to scientists in intersecting fields and in the development of harmonised approaches.

Characterization of a Whole Smoke In Vitro Exposure System (Burghart Mimic Smoker-01)

2009 ◽  
Vol 21 (3) ◽  
pp. 234-243 ◽  
Author(s):  
Mariano J. Scian* ◽  
Michael J. Oldham ◽  
David B. Kane ◽  
Jeffery S. Edmiston ◽  
Willie J. McKinney
Keyword(s):  
Exposure System ◽  
In Vitro Exposure ◽  
Whole Smoke

Development of a numerical model connecting electromagnetism, thermal and hydrodynamics to analyse in vitro exposure system

2008 ◽  
Vol 63 (1-2) ◽  
pp. 17-28 ◽  
Author(s):  
Marylène Cueille ◽  
Alice Collin ◽  
Claudine Pivain ◽  
Philippe Leveque
Keyword(s):  
Numerical Model ◽  
Exposure System ◽  
In Vitro Exposure

scholarly journals In vitro exposure system for study of aerosolized influenza virus

Virology ◽  
2017 ◽  
Vol 500 ◽  
pp. 62-70 ◽  
Author(s):  
Hannah M. Creager ◽  
Hui Zeng ◽  
Joanna A. Pulit-Penaloza ◽  
Taronna R. Maines ◽  
Terrence M. Tumpey ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Exposure System ◽  
In Vitro Exposure

scholarly journals Evaluation of Dicarbonyls Generated in a Simulated Indoor Air Environment Using an In Vitro Exposure System

2010 ◽  
Vol 115 (2) ◽  
pp. 453-461 ◽  
Author(s):  
Stacey E. Anderson ◽  
Laurel G. Jackson ◽  
Jennifer Franko ◽  
J. R. Wells
Keyword(s):  
Indoor Air ◽  
Exposure System ◽  
In Vitro Exposure

scholarly journals Validation of an in vitro exposure system for toxicity assessment of air-delivered nanomaterials

2013 ◽  
Vol 27 (1) ◽  
pp. 164-173 ◽  
Author(s):  
Jong Sung Kim ◽  
Thomas M. Peters ◽  
Patrick T. O’Shaughnessy ◽  
Andrea Adamcakova-Dodd ◽  
Peter S. Thorne
Keyword(s):  
Toxicity Assessment ◽  
Exposure System ◽  
In Vitro Exposure

scholarly journals The Accordion and the Deep Bowl of Spaghetti: Eight Researchers' Experiences of Using IPA as a Methodology

2014 ◽  
Author(s):  
Chris Wagstaff ◽  
Hyeseung Jeong ◽  
Maeve Nolan ◽  
Tony Wilson ◽  
Julie Tweedlie ◽  
...  
Keyword(s):  
Mental Health ◽  
Second Language Acquisition ◽  
Child Protection ◽  
Interpretative Phenomenological Analysis ◽  
In Vitro Fertilisation ◽  
Mental Health Nursing ◽  
Cord Injury ◽  
The Usa ◽  
The Uk

Since 1996 Interpretative Phenomenological Analysis (IPA) has grown rapidly and been applied in areas outside its initial “home” of health psychology. However, explorations of its application from a researcher's perspective are scarce. This paper provides reflections on the experiences of eight individual researchers using IPA in diverse disciplinary fields and cultures. The research studies were conducted in the USA, Malaysia, Australia, New Zealand, Ireland and the UK by researchers with backgrounds in business management, consumer behaviour, mental health nursing, nurse education, applied linguistics, clinical psychology, health and education. They variously explored media awareness, employee commitment, disengagement from mental health services, in-vitro fertilisation treatment, student nurses' experience of child protection, second language acquisition in a university context, the male experience of spinal cord injury and academics experience of working in higher education and women’s experiences of body size and health practices. By bringing together intercultural, interdisciplinary experiences of using IPA, the paper discusses perceived strengths and weaknesses of IPA.

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