scholarly journals The Red Cell Chromium Elution Rate in Patients with Some Hematologic Diseases

Blood ◽  
1963 ◽  
Vol 21 (1) ◽  
pp. 63-69 ◽  
Author(s):  
MARTIN J. CLINE ◽  
NATHANIEL I. BERLIN
Keyword(s):  
Life Span ◽  
Rate Constant ◽  
Rate Constants ◽  
Red Cell ◽  
Hematologic Diseases ◽  
Cell Life ◽  
Elution Rate ◽  
Single Exponential

Abstract 1. The erythrocyte Cr51 elution rate was determined in 38 patients with hematologic diseases. 2. In four patients with finite red cell life spans, two exponential Cr51 elution rate constants could be calculated. In the remaining 34 patients, the data were consistent with a single exponential elution rate constant from day 1 to day 30-40 following Cr51 administration. 3. The single elution rate varied from 0.62 to 2.27 per cent per day. 4. In two patients, the chromium elution rates determined on two separate occasions were not significantly different. In a third individual, the chromium elution rate constant was 0.75 per cent per day when the red cell life span was 66 days and 1.07 per cent per day when red cell life span was 79 days.

scholarly journals 32DFP and 51Cr for Measurement of Red Cell Life Span in Abnormal Hemoglobin Syndromes

Blood ◽  
1969 ◽  
Vol 33 (2) ◽  
pp. 214-224 ◽  
Author(s):  
PAUL R. MCCURDY
Keyword(s):  
Life Span ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Cells ◽  
Red Cell ◽  
Daily Range ◽  
Hemoglobin C ◽  
Cell Life ◽  
Abnormal Hemoglobin ◽  
Elution Rate

Abstract The red cell life span was measured simultaneously using 51Cr and 32DFP in 21 patients with abnormal hemoglobin β chains and in 7 patients with normal hemoglobin. The patients included 9 with sickle cell anemia, 2 with sickle-β-thalassemia disease, 2 with sickle cell-hemoglobin C disease, 3 with homozygous hemoglobin C disease, 4 with sickle cell trait and 1 with hemoglobin C trait. The 51Cr elution rate from red cells carrying abnormal β chains was 1.2 per cent daily (range: 0.1-2.7 per cent; 4 patients had 2-component elution curves, the first of which was quite rapid (4.1-19.5 per cent daily) and could lead to significant error in the 51Cr estimate of red cell survival. The 51Cr elution rate for red cells with normal β chains was 1.3 per cent daily (range: 0.7-1.6 per cent). In the steady state, production of red cells was estimated from the 32DFP life span. Both figures varied with the disease process but erythropoiesis seldom obtained the 6-8 fold increase over normal that is considered to be the capability of normal bone marrow and hence a relative erythropoietic defect seems to be frequently present in patients with abnormal hemoglobin diseases. Two sibling pairs with sickle cell anemia were studied and were found to vary from each other as much as did the other patients with this disorder.

Red cell life span of white-tailed deer Odocoileus virginianus

1966 ◽  
Vol 19 (2) ◽  
pp. 471-473 ◽  
Author(s):  
Ward D Noyes ◽  
Hyram Kitchen ◽  
W Jape Taylor
Keyword(s):  
Life Span ◽  
Odocoileus Virginianus ◽  
Red Cell ◽  
Cell Life

scholarly journals The Red Cell Chromium Elution Rate in Patients with Some Hematologic Diseases

Blood ◽  
1963 ◽  
Vol 21 (3) ◽  
pp. 402-402 ◽  
Author(s):  
MARTIN J. CLINE ◽  
NATHANIEL I. BERLIN
Keyword(s):  
Red Cell ◽  
Hematologic Diseases ◽  
Elution Rate

Abstract In the paper entitled "The Red Cell Chromium Elution Rate in Patients with Some Hematologic Diseases," by Martin J. Cline and Nathaniel I. Berlin (January BLOOD, pp. 63-69), equation (4), page 64, was printed incorrectly. The equation should read: See PDF for Equation

THE EFFECT OF SULPHAEMOGLOBIN ON RED CELL VIABILITY

1957 ◽  
Vol 35 (1) ◽  
pp. 1171-1181
Author(s):  
L. G. Israels ◽  
A. Chutorian ◽  
G. E. Delory ◽  
Esther Israels
Keyword(s):  
Cell Viability ◽  
Life Span ◽  
Cell Survival ◽  
Red Cells ◽  
Red Cell ◽  
Disappearance Rate ◽  
Cell Life ◽  
Red Cell Survival ◽  
Calcium Sulphide ◽  
True Measure

Sulphaemoglobinaemia was produced in rabbits by the injection of para-aminopropriophenone and calcium sulphide. The disappearance of this pigment from the blood was used as an index of red cell survival. Sulphaemoglobin disappeared in an exponential fashion, indicating a mean red cell life span of 36 days. The red cells were also tagged with Cr51, and this method of measuring erythrocyte life span yielded values strongly suggesting that sulphaemoglobin in the red cell impairs its viability and leads to random cell destruction. Under these conditions it would seem that the disappearance rate of sulphaemoglobin is not a true measure of red cell survival.

Quantitative measurement of erythropoiesis in the dog

1960 ◽  
Vol 198 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S. M. Weissman ◽  
T. A. Waldmann ◽  
N. I. Berlin
Keyword(s):  
Life Span ◽  
Cell Survival ◽  
Cell Volume ◽  
Quantitative Measurement ◽  
Red Cells ◽  
Red Cell ◽  
Red Cell Volume ◽  
Cell Life ◽  
Red Cell Survival

The quantitative measurement of erythropoiesis requires the simultaneous determination of total red cell volume, rate of production of red cells and the red cell life span. The total red cell volume was measured with autologous Cr51-labeled red cells, the rate of production of red cells from the rate of disappearance of radioiron from the plasma and uptake by red cells, the red cell life span with C14-labeled glycine and the apparent red cell survival T1/2 with Cr51. The average total red cell volume of the dogs studied was 38.6 cc/kg; the plasma radioiron T1/2 was 66 minutes; the red cell radio-iron uptake was 80%; the serum iron was 102 µg/100 cc, and the plasma volume calculated from the peripheral hematocrit and total red cell volume was 46 cc/kg, and from the extrapolation to t0 of the radioiron disappearance was 48 cc/kg. From these figures the plasma iron turnover was calculated to be 0.63 mg/kg/day and the red cell iron renewal rate 1.26%/day. The average red cell life span was 108 days; the average apparent T1/2 of Cr51 red cell survival was 24.3 days; the average elution rate of Cr51 was 1.77%/day.

scholarly journals Neutrophil life span in paroxysmal nocturnal hemoglobinuria

Blood ◽  
1977 ◽  
Vol 50 (4) ◽  
pp. 657-662 ◽  
Author(s):  
LH Brubaker ◽  
LJ Essig ◽  
CE Mengel
Keyword(s):  
Life Span ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Neutropenic Patient ◽  
Red Cell ◽  
Fresh Serum ◽  
Cell Life ◽  
Sufficient Degree ◽  
Different Sources ◽  
Active Complement

Abstract We have studied neutrophil intravascular life span in six patients with paroxysmal nocturnal hemoglobinuria (PNH); four had normal neutrophil counts when studied and two were neutropenic. Five patients had enough circulating neutrophils to isolate for tests in vitro. Lysis of labeled neutrophils was greatly increased, compared to that of normal volunteers, when these neutrophils were incubated with acidified fresh serum as a source of active complement plus serum containing antineutrophil antibodies (from three different sources). Despite the in vitro lesion, however, each of these patients had a normal neutrophil intravascular life span as measured by the 32P- diisopropylfluorophosphate technique. One neutropenic patient, who had a normal neutrophil life span, had a shift of cells from the circulating to marginated pool of sufficient degree to cause the neutropenia. A second (severely) neutropenic patient was found to have developed extreme marrow hypoplasia, also explaining the neutropenia. Thus, in contrast to the shortened red cell life span, we have been unable to find a shortened neutrophil life span in PNH.

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