Chromosome studies in adult T-cell leukemia in Japan: significance of trisomy 7

Abstract Chromosomes were studied in cells from 15 japanese patients with adult T-cell leukemia (ATL). Mitoses were obtained from unstimulated peripheral blood in 12 patients and a lymph node in one patient. In two other patients, mitotic cells were obtained solely from peripheral blood stimulated with phytohemagglutinin (PHA). Chromsomally abnormal cells were seen in 14 of the 15 patients. The abnormal cells had a modal number of chromosomes in near diploid range in 12 patients, and in near triploid and tetraploid range in the remaining 2 patients, respectively. Eight of the nine patients analyzed by Q-banding had clonal chromosome abnormalities. The most common abnormality was trisomy no.7 or 7q, which was seen in 5 cases and has been primarily observed in lymphoid neoplasms. A 14q+ marker chromosome was found in two patients and a Dq+ in one patient; loss of a sex chromosome was found in three patients. Most chromosomes were involved in gains, losses, or structural rearrangements, but abnormalities of no. 11, which have been frequently found in lymphoid malignancies, was not observed in our series. The significance of these chromosome abnormalities is discussed.

Download Full-text

Chromosome studies in adult T-cell leukemia in Japan: significance of trisomy 7

Blood ◽

10.1182/blood.v58.3.420.bloodjournal583420 ◽

1981 ◽

Vol 58 (3) ◽

pp. 420-425 ◽

Cited By ~ 1

Author(s):

Y Ueshima ◽

S Fukuhara ◽

T Hattori ◽

T Uchiyama ◽

K Takatsuki ◽

...

Keyword(s):

T Cell ◽

Peripheral Blood ◽

Sex Chromosome ◽

Marker Chromosome ◽

Japanese Patients ◽

Chromosome Abnormalities ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Adult T Cell Leukemia ◽

Abnormal Cells

Chromosomes were studied in cells from 15 japanese patients with adult T-cell leukemia (ATL). Mitoses were obtained from unstimulated peripheral blood in 12 patients and a lymph node in one patient. In two other patients, mitotic cells were obtained solely from peripheral blood stimulated with phytohemagglutinin (PHA). Chromsomally abnormal cells were seen in 14 of the 15 patients. The abnormal cells had a modal number of chromosomes in near diploid range in 12 patients, and in near triploid and tetraploid range in the remaining 2 patients, respectively. Eight of the nine patients analyzed by Q-banding had clonal chromosome abnormalities. The most common abnormality was trisomy no.7 or 7q, which was seen in 5 cases and has been primarily observed in lymphoid neoplasms. A 14q+ marker chromosome was found in two patients and a Dq+ in one patient; loss of a sex chromosome was found in three patients. Most chromosomes were involved in gains, losses, or structural rearrangements, but abnormalities of no. 11, which have been frequently found in lymphoid malignancies, was not observed in our series. The significance of these chromosome abnormalities is discussed.

Download Full-text

Adult T-Cell Leukemia/Lymphoma in a Peripheral Blood Smear

Archives of Pathology & Laboratory Medicine ◽

10.5858/2003-127-0636-atliap ◽

2003 ◽

Vol 127 (5) ◽

pp. 636-636

Author(s):

Dragos C. Luca ◽

Carey Z. August ◽

Elliot Weisenberg

Keyword(s):

T Cell ◽

Peripheral Blood ◽

Blood Smear ◽

Peripheral Blood Smear ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Adult T Cell Leukemia

Download Full-text

Entry of adult T-cell leukemia-associated virus into human peripheral blood leukocytes

Archives of Virology ◽

10.1007/bf01314241 ◽

1985 ◽

Vol 85 (3-4) ◽

pp. 321-328 ◽

Cited By ~ 2

Author(s):

T. Goto ◽

K. Sugita ◽

S. Imura ◽

K. Sano ◽

M. Nakai

Keyword(s):

T Cell ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Adult T Cell Leukemia

Download Full-text

Chromosome aberrations in T lymphocytes carrying adult T-cell leukemia- associated antigens (ATLA) from healthy adults

Blood ◽

10.1182/blood.v61.1.205.205 ◽

1983 ◽

Vol 61 (1) ◽

pp. 205-207 ◽

Cited By ~ 18

Author(s):

S Fukuhara ◽

Y Hinuma ◽

YI Gotoh ◽

H Uchino

Keyword(s):

T Cell ◽

T Lymphocytes ◽

Chromosome Aberrations ◽

Unknown Origin ◽

Healthy Adults ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Adult T Cell Leukemia ◽

The Third ◽

Abnormal Cells

Abstract Chromosomes were studied in cultured T lymphocytes carrying adult T- cell leukemia-associated antigens (ATLA) that were obtained from five Japanese anti-ATLA seropositive healthy adults. Chromosomally abnormal cells were observed in three of the five healthy adults, and these cells were clonal in two subjects. All cells examined in one subject had rearrangements of chromosome nos. 7 and 14. Clonal cells from the second had a minute chromosome of unknown origin. A few cells in the third had nonclonal rearrangements of chromosomes. Thus, ATLA-positive T lymphocytes in some anti-ATLA seropositive healthy people have chromosome aberrations.

Download Full-text

Correlation of chromosome abnormalities with histologic and immunologic characteristics in non-Hodgkin's lymphoma and adult T cell leukemia- lymphoma. Fifth International Workshop on Chromosomes in Leukemia- Lymphoma

Blood ◽

10.1182/blood.v70.5.1554.1554 ◽

1987 ◽

Vol 70 (5) ◽

pp. 1554-1564 ◽

Cited By ~ 105

Keyword(s):

United States ◽

T Cell ◽

International Workshop ◽

Chromosome Abnormalities ◽

Cell Phenotype ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Adult T Cell Leukemia ◽

Trisomy 12 ◽

Trisomy 3

Abstract To correlate cytogenetic findings with histology and immunophenotype, 260 newly diagnosed patients with non-Hodgkin's lymphoma (NHL) and 31 with adult T cell leukemia/lymphoma (ATL) were studied at the Fifth International Workshop on Chromosomes in Leukemia-Lymphoma. Clonal chromosome abnormalities were found in 85% of cases of NHL and in all cases of ATL. The most significant associations of histology with cytogenetics involved the 8q24 and 14;18 translocations. Of 23 NHL patients with an 8q24 translocation, 20 (87%) had small noncleaved cell (SNC) lymphoma. Of 57 NHL patients with a t(14;18)(q32;q21), 37 (65%) had a follicular lymphoma, and at least 13 others (23%) had a diffuse lymphoma of follicular center cell origin. Five others including two who also had an 8q24 translocation had SNC lymphoma; tumors in these patients lacked the monomorphic features seen in those with only an 8q24 translocation. The most striking associations of immunology with cytogenetics involved rearrangements of 14q11–13, abnormalities of 1p, trisomy 3, and trisomy 12. Abnormalities of 14q11–13 were correlated with T cell disease. Among 138 NHL and 24 ATL cases that lacked involvement of 8q24, t(14;18), or rearrangements of 14q11–13, abnormalities of 1p and trisomy 3 were significantly associated with the T cell phenotype (P less than .01) and trisomy 12 with the B cell phenotype (P less than .01). No karyotype difference was found between Japanese ATL and non-Japanese T cell lymphomas except that +3 occurred more often in the former (P = .06). Although the 8q24 translocations in 23 patients were distributed relatively equally among geographic regions (United States, ten; Europe, ten; Japan, three), the t(14;18) in 57 patients was much less frequently seen in Japan than in the other parts of the world (United States, 31; Europe, 21; Australia, four; Japan, one). Thirty-nine chromosome bands had breaks in five or more cases; a number of protooncogenes and genes related to lymphocyte function located in these bands are likely to be involved in the development of lymphoma. Thus, besides confirming the close association of particular chromosome abnormalities with histology, we have identified a number of new associations that merit molecular analysis.

Download Full-text

Clinical and in vitro resistance to bexarotene in adult T-cell leukemia: loss of RXR-α receptor

Blood ◽

10.1182/blood-2008-03-141424 ◽

2008 ◽

Vol 112 (6) ◽

pp. 2484-2488 ◽

Cited By ~ 7

Author(s):

Julie H. Lin ◽

Ellen J. Kim ◽

Anand Bansal ◽

John Seykora ◽

Stephen K. Richardson ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Peripheral Blood ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Adult T Cell Leukemia ◽

First Case ◽

Induced Apoptosis ◽

Resistant Cells

Abstract The oral rexinoid bexarotene (Targretin) is widely used for treatment of cutaneous T-cell lymphomas (CTCL). We recently reported the first case of adult T-cell leukemia/lymphoma (ATLL) that responded rapidly to combination therapy of bexarotene and interferon (IFN)-α2b with complete clinical response. We demonstrated that bexarotene induced apoptosis of the patient's malignant peripheral blood T-cells in vitro. However, our patient developed skin and nodal relapse 180 days after starting treatment. We now demonstrate that his peripheral blood malignant T cells became resistant to bexarotene-induced apoptosis. We investigated potential mechanisms that may cause aberrations in the retinoid X receptor (RXR) subunits, RXR-α and RXR-β, to account for these findings. Sequence analysis did not reveal acquisition of mutations in the genes encoding RXR-α and RXR-β by resistant cells. We assessed RXR-α and RXR-β expression by Western blot analysis and found that resistant cells had significantly decreased RXR-α expression compared with pretherapy bexarotene-sensitive cells. Our findings indicate that reduced expression of the RXR-α receptor subunit may represent a mechanism for resistance to bexarotene in T-cell malignancies.

Download Full-text