scholarly journals Intact transferrin receptors in human plasma and their relation to erythropoiesis

Blood ◽  
1990 ◽  
Vol 75 (1) ◽  
pp. 102-107 ◽  
Author(s):  
HA Huebers ◽  
Y Beguin ◽  
P Pootrakul ◽  
D Einspahr ◽  
CA Finch
Keyword(s):  
Human Plasma ◽  
Polyclonal Antibodies ◽  
Close Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Transferrin Receptors ◽  
Erythroid Hyperplasia ◽  
The Mean ◽  
Tissue Receptors ◽  
Mean Concentration ◽  
Normal Adults

Abstract Intact transferrin receptor molecules complexed with transferrin were found in human plasma. The concentration of receptors was determined by an enzyme-linked immunosorbent assay that uses polyclonal antibodies. The mean concentration of 8,279 micrograms/L in 56 normal adults appears to be unrelated to age or sex. Additional receptor measurements were performed on plasmas from 260 subjects with erythropoietic disorders. Decreased concentration of plasma receptors was found in patients with erythroid hypoplasia and increased numbers in those with erythroid hyperplasia. Ferrokinetic measurements of erythropoiesis were compared with numbers of receptors in 148 subjects, and a close correlation was found (r = .86). Both sets of values, measured in different conditions and expressed in relation to normal, were consistent with expected values. Receptor values were unproportionally increased only in conditions of iron deficiency. It is concluded that plasma receptors have a constant relationship to tissue receptors, and their number in most instances reflects the rate of erythropoiesis.

scholarly journals Intact transferrin receptors in human plasma and their relation to erythropoiesis

Blood ◽  
1990 ◽  
Vol 75 (1) ◽  
pp. 102-107 ◽  
Author(s):  
HA Huebers ◽  
Y Beguin ◽  
P Pootrakul ◽  
D Einspahr ◽  
CA Finch
Keyword(s):  
Human Plasma ◽  
Polyclonal Antibodies ◽  
Close Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Transferrin Receptors ◽  
Erythroid Hyperplasia ◽  
The Mean ◽  
Tissue Receptors ◽  
Mean Concentration ◽  
Normal Adults

Intact transferrin receptor molecules complexed with transferrin were found in human plasma. The concentration of receptors was determined by an enzyme-linked immunosorbent assay that uses polyclonal antibodies. The mean concentration of 8,279 micrograms/L in 56 normal adults appears to be unrelated to age or sex. Additional receptor measurements were performed on plasmas from 260 subjects with erythropoietic disorders. Decreased concentration of plasma receptors was found in patients with erythroid hypoplasia and increased numbers in those with erythroid hyperplasia. Ferrokinetic measurements of erythropoiesis were compared with numbers of receptors in 148 subjects, and a close correlation was found (r = .86). Both sets of values, measured in different conditions and expressed in relation to normal, were consistent with expected values. Receptor values were unproportionally increased only in conditions of iron deficiency. It is concluded that plasma receptors have a constant relationship to tissue receptors, and their number in most instances reflects the rate of erythropoiesis.

Quantification of serum amyloid P by enzyme-linked immunosorbent assay

1991 ◽  
Vol 37 (10) ◽  
pp. 1742-1745 ◽  
Author(s):  
R Saïle ◽  
A Kandoussi ◽  
M Deveaux ◽  
J Descamps ◽  
E Hachulla ◽  
...  
Keyword(s):  
Human Plasma ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Serum Amyloid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Amyloid P ◽  
Assay Procedure ◽  
Normal Individuals ◽  
Sandwich Type ◽  
Amyloid P

Abstract This enzyme-linked immunosorbent assay procedure for quantifying serum amyloid P (SAP) in human plasma makes use of affinity-purified polyclonal antibodies to SAP in a "sandwich"-type format. The procedure is sensitive, reproducible, simple, and easily automatable. Results correlate well with those by a rocket immunoelectrophoresis method performed with the same antibodies. Sera from apparently normal individuals had a mean SAP content of 44.17 mg/L and increased with age.

scholarly journals An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

2012 ◽  
Vol 1 (2) ◽  
pp. 93-97
Author(s):  
Vinay Vadvadgi ◽  
Neeta Padmawar
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Mean Concentration

ABSTRACT Background and objective Plasma leptin is associated in patients with inflammatory diseases. A high concentration of leptin is associated with healthy gingival tissue. The purpose of this study was to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum within healthy and diseased gingiva, further to explore the possibility of using the levels of leptin in GCF and serum as a biochemical marker of periodontal disease progression. Materials and methods Ninety subjects were selected with age (30-39 years) and sex (15 males and 15 females) matched, to eliminate age and sex as confounders. The subjects were divided into three groups consisting of 30 subjects in each group based on the clinical and radiological parameters; healthy (group I), gingivitis (group II), periodontitis (group III), from whom the GCF samples were collected with Periopaper GCF collection strips (Proflow, Amityville, NY, USA) for 30 seconds and blood samples with 20-gauge needle syringe respectively. Leptin concentration was determined from individual GCF and serum samples by enzyme-linked immunosorbent assay (ELISA). Results The highest mean leptin concentration in GCF was observed in group I (2,664.30 pg/ml ± 324.73) and least mean leptin concentration was obtained in group III (1,309.43 pg/ml ± 202.45). The mean concentration of group II (1,639.43 pg/ml ± 344.46) was intermediate between the highest and lowest values. In contrast, the highest mean leptin concentration in serum was obtained for group III (12,086.57 pg/ml ± 1,698.23) and least mean leptin concentration was obtained for group I (8,715.09 pg/ml ± 1,649.19). The mean concentration of the group II (10,694.01 pg/ml ± 1,777.72) were intermediate between the highest and lowest values. Conclusion The results indicated a statistically significant decrease in the GCF leptin concentration and increase in serum leptin concentration as the periodontal disease progressed. How to cite this article Vadvadgi VH, Saini R, Padmawar N. An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis. Int J Experiment Dent Sci 2012;1(2):93-97.

Time-resolved immunofluorometric assay of 17 beta-hydroxysteroid dehydrogenase in plasma

1991 ◽  
Vol 37 (8) ◽  
pp. 1412-1415 ◽  
Author(s):  
O Mäentausta ◽  
M Menjivar ◽  
R Vihko
Keyword(s):  
Polyclonal Antibodies ◽  
Endometrial Adenocarcinoma ◽  
Plasma Concentrations ◽  
Hydroxysteroid Dehydrogenase ◽  
Minimum Detectable Concentration ◽  
Antibody Molecule ◽  
Time Resolved ◽  
Detectable Concentration ◽  
The Mean ◽  
Mean Concentration

Abstract We describe a time-resolved immunofluorometric assay (TR-IFMA) for human 17 beta-hydroxysteroid dehydrogenase (17HSD) in which antibody-coated microtiter strip wells and europium chelate-labeled polyclonal antibodies are used. In preparing the label, a polyclonal antibody is affinity-purified and derivatized with diethylenetriamine-pentaacetic acid. With this derivative, five to eight europium ions can be combined with one antibody molecule without decreasing the antibody's immunoreactivity. The minimum detectable concentration of 17HSD is 0.13 microgram/L; the intra- and interassay CVs are less than 8% and less than 15%, respectively, for concentrations between 0.3 and 100 micrograms/L. There is no difference between the concentrations of 17HSD in plasma specimens taken during the proliferative and luteal phases of the menstrual cycle, the measured mean concentration being 0.22 microgram/L. We found no correlation between plasma 17HSD and progesterone concentrations. The plasma concentrations of 17HSD increase during pregnancy, the mean concentrations being 1.5, 4.4, and 12.5 micrograms/L, during the first, second, and third trimesters of pregnancy, respectively. In the specimens from 18 men, the mean concentration was 0.18 microgram/L. In six plasma specimens from patients with endometrial adenocarcinoma, the mean concentration was 0.20 micrograms/L. Pre-analytical aspects are important in the assay of 17HSD because of the lability of the enzyme protein. Preferably, blood should be sampled into EDTA-containing tubes, plasma should be separated within 15 min, and glycerol must be added without delay to a final volume of 200 mL/L.

scholarly journals Novel Enzyme-Linked Immunoassay To Determine Nanogram Levels of Pneumocandins in Human Plasma

1998 ◽  
Vol 36 (5) ◽  
pp. 1414-1418 ◽  
Author(s):  
Y. D. Karkhanis ◽  
D. M. Schmatz
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
Human Plasma ◽  
Antifungal Agents ◽  
Polyclonal Antibodies ◽  
Guanidine Hydrochloride ◽  
Microtiter Plate ◽  
Water Soluble ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Candidate

A binding enzyme-linked immunosorbent assay (ELISA) has been developed for measuring nanogram concentrations of semisynthetic pneumocandin antifungal agents in human plasma. Semisynthetic pneumocandin L-733,560 was conjugated to succinylated hemocyanin by water-soluble carbodiimide and was used as an immunogen to produce polyclonal antibodies in rabbits. Pneumocandins were used to directly coat the wells of a microtiter plate, and quantitation was achieved by using rabbit polyclonal antibodies to pneumocandin L-733,560 and goat anti-rabbit immunoglobulin G conjugated to either alkaline phosphatase or horseradish peroxidase. Maximum binding of L-733,560 and most related analogs to the wells of the microtiter plate was found to occur in the first 5 min of incubation at 4°C. Once bound to the plate, these pneumocandins could not be removed from the plate, either by treatment with 4.0 to 6.0 M urea or by treatment with 4.0 to 6.0 M guanidine hydrochloride for 24 h at 4°C. The binding ELISA is linear with drug concentration and can detect levels of L-733,560 as low as 5 ng/ml in human plasma. The assay is also useful for quantitating plasma levels of related semisynthetic pneumocandins including clinical candidate MK-0991.

A New Radioimmunoassay-Technique For Fibrinopeptide-A In Human Plasma

1979 ◽  
Author(s):  
J Harenberg ◽  
R Zimmermann ◽  
F Haas ◽  
H Schmidt-Gayk
Keyword(s):  
Myocardial Infarction ◽  
Venous Thrombosis ◽  
Human Plasma ◽  
Original Method ◽  
Fibrinopeptide A ◽  
Microtiter Plates ◽  
Antibody Method ◽  
The Mean ◽  
Mean Concentration ◽  
Healthy Persons

Fibrinopeptide-A (FpA) is thought to be the most sensitive parameter to indicate hypercoagulability with increased fibrin formation in man. Previously described radioimmunoassays for FpA are very time consuming. We present therefore a modification of the assay, which is less time consuming, sensitive, reproducable and reliable. The following modifications were made on the original method: 1. plasma dialysis was omitted 2. performance on microtiter-plates 3. double antibody method.The sensitivity was improved to 0.16 ng FpA / ml plasma. The mean concentration of FpA in dialysed and undialysed plasma did not differ significantly (p<0.001). Effective separation of fibrinogen was achieved by aethanol extraction alone. The mean concentration of FpA correlated highly, when the tracer was separated by charcoal or second antibody (r = 0.96). The recovery was improved to 85%.In healthy persons 0.16-2.5 ng FpA/ml plasma were measured. In patients with venous thrombosis or myocardial infarction in the history FpA was elevated significantly to 2.0 ng/ml-19.6 ng/ml, indicating that the FpA is a usefull tool in diagnosis of hypercoagulability.

Cross-reactivity of monomeric and dimeric biosynthetic human growth hormone in commercial immunoassays

1990 ◽  
Vol 36 (2) ◽  
pp. 362-366 ◽  
Author(s):  
R R Bowsher ◽  
J M Apathy ◽  
A L Ferguson ◽  
R M Riggin ◽  
D P Henry
Keyword(s):  
Growth Hormone ◽  
Model Compound ◽  
Rank Order ◽  
Polyclonal Antibodies ◽  
Human Growth ◽  
Cross Reactivity ◽  
Serum Samples ◽  
The Mean ◽  
Commercial Kits ◽  
Normal Adults

Abstract Commercial kits give different measurements for concentrations of growth hormone (GH, somatotropin) in serum. Most notably, a two-site monoclonal-antibody-based immunoradiometric assay (IRMA) from Hybritech routinely yields lower values than do conventional RIAs in which polyclonal antibodies are used. We used purified dimeric biosynthetic human GH as a model compound to investigate the specificity of five commercial immunoassays for size variants of GH. In all five assays, biosynthetic monomeric GH was significantly more potent than pituitary-derived standard GH supplied with the kits. Dimeric GH was significantly less potent than monomer in four of the five assays, and cross-reactivities varied more than fivefold, from 15% to 84%. Using three commercial kits selected for their specificity for dimeric GH, we measured GH in serum samples from 18 normal adults. The mean GH concentrations in serum--0.7 (Hybritech, IRMA), 1.8 (Diagnostic Products, RIA), and 3.1 (Cambridge, RIA) micrograms/L--differed significantly, but in the same rank order as that obtained in the experiments on dimer cross-reactivity.

Radioimmunoassay of atrial natriuretic polypeptide in heat-treated human plasma.

1987 ◽  
Vol 33 (5) ◽  
pp. 674-676 ◽  
Author(s):  
K Iinuma ◽  
I Ikeda ◽  
T Ogihara ◽  
H Hara ◽  
J Shima ◽  
...  
Keyword(s):  
Heart Failure ◽  
Human Plasma ◽  
Salt Intake ◽  
Atrial Natriuretic Polypeptide ◽  
Heat Treated ◽  
High Salt Intake ◽  
The Mean ◽  
High Concentrations ◽  
Mean Concentration ◽  
Atrial Natriuretic

Abstract In this simple, sensitive radioimmunoassay (RIA) of atrial natriuretic polypeptide (hANP) in human plasma, nonspecific interference is minimized by deproteinizing the plasma by heat treatment at 85 degrees C for 10 min. We directly measure alpha-hANP in the supernates by RIA, with use of antiserum that recognizes the N-terminal region of alpha-hANP. The minimal detectable value was 0.4 pg per tube. The intra-assay CV was 6.6% (n = 8). The mean concentration of hANP in plasma of 54 healthy volunteers was 41 (SD 29) ng/L. Concentrations of hANP in plasma increased after saline infusion and high salt intake for one week in patients with essential hypertension. High concentrations were also measured in patients with renal failure and congestive heart failure. This method, which requires no extraction or purification with column chromatography, is especially useful for simultaneous measurement of several samples.

THE BINDING OF CORTISOL BY OVINE PLASMA PROTEINS

1967 ◽  
Vol 37 (3) ◽  
pp. 261-268 ◽  
Author(s):  
J. Y. F. PATERSON ◽  
F. HILLS
Keyword(s):  
Human Plasma ◽  
Plasma Proteins ◽  
Binding Capacity ◽  
Equilibrium Dialysis ◽  
Affinity Constant ◽  
Albumin Solution ◽  
Plasma Albumin ◽  
The Mean ◽  
Mean Concentration

SUMMARY Albumin was isolated from ovine plasma and its affinity for cortisol was determined by equilibrium dialysis at 37°. The value of Ka[σpa] for a 1 % (w/v) albumin solution was 0·275 which is similar to the value for human plasma albumin. The affinity constant of transcortin in ovine plasma was determined by equilibrium dialysis of diluted plasma at several concentrations of cortisol. The value found, Kt (37°) = 0·87 x 108 l./mole, is close to that found for human plasma transcortin by Mills (1962). The concentration of transcortin in ovine plasma, expressed as cortisolbinding capacity, was 6–49 μg. (mean 24 μg.) cortisol/l. These concentrations are much lower than those found in human plasma. The observation of Lindner (1964) that cortisol binding capacity did not increase during pregnancy in sheep has been confirmed. In sheep which were accustomed to handling, the mean concentration of cortisol in plasma was 17·8 μg./l. and of this amount 59% was bound to transcortin, 19 % to albumin and 22 % was not bound to protein.

scholarly journals Heavy metal contamination and antibiotic residues in poultry feed and meat in Bangladesh

2021 ◽  
Vol 5 (2) ◽  
pp. 71-78
Author(s):  
Md Mahbubul Alam ◽  
Dwijendra Lal Mallick ◽  
Md Murshidul Ahsan ◽  
AHM Taslima Akhter ◽  
- Eftesum ◽  
...  
Keyword(s):  
Poultry Feed ◽  
Enzyme Linked Immunosorbent Assay ◽  
Meat Sample ◽  
Poultry Production ◽  
Antibiotic Residues ◽  
The Mean ◽  
Meat Samples ◽  
Local Farm ◽  
Mean Concentration ◽  
Feed Samples

Presence of harmful contaminants and residues in poultry feed and meat have serious public health consequence. This study was carried out to identify and quantify antibiotic residues, heavy metals and toxins in poultry feed and meat in the two selected poultry production belts of Bangladesh. A total of 94 broiler feed samples and 60 broiler meat samples were collected and tested by Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC) and Enzyme-linked Immunosorbent Assay (ELISA) for identification and quantification of the parameters. Antibiotic residues were detected in 18.89% of the feed samples, whereas, there were no toxin (Aflatoxin) positive samples. Among the antibiotic positive samples, Oxytetracycline (OTC) was found predominant and detected in 12.22% cases. The mean concentrations of Cadmium (Cd), Lead (Pb), Chromium (Cr) were found as 0.04 mg/kg, 1.28 mg/kg and 2.55 mg/kg respectively in feed samples. In the case of meat samples, the mean concentration of OTC, Ciprofloxacin (CIP), and Tetracycline (TCL) residues were found 8.67 ppb, 7.18 ppb and 0.81 ppb accordingly. The highest mean concentration of Oxytetracycline (OTC) (10.15 ppb) was found in samples collected from local poultry sellers, whereas, the highest mean concentration of Tetracycline (TCL) (1.35 ppb) and Ciprofloxacin (CIP) (10.62 ppb) were observed in the samples obtained from local farm. The highest percentage of TCL and CIP (64% and 48% respectively) were found in samples collected from local farm. Chlortetracycline (CTC) was found predominant (70%) in samples collected from Contract farms. On the other hand, out of 60 meat samples, Cd and Cr were detected in only one meat sample with concentration of 56.41 mg/kg and 14.44 mg/kg respectively. Lead was not detected in any of the meat samples. Asian Australas. J. Food Saf. Secur. 2021, 5 (2), 71-78

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