Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP

In the course of an experiment in malaria control in an inland region of Kenya and Tanganyika, by the use of house spraying with dieldrin, routine catches were maintained of mosquitos resting in artificial outdoor shelters. During the 18 months of the pre-spraying period, catches in the South Pare district of Tanganyika mainly consisted of the principal vectors, Anopheles gambiae Giles and A. funestus Giles, together with small numbers of A. rivulorum Leeson.During the three years following the spraying, A. funestus disappeared almost completely from the catches, while A. rivulorum showed an increase of about seven times above its former level.

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Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCR-RFLP

Zootaxa ◽

10.11646/zootaxa.1310.1.3 ◽

2006 ◽

Vol 1310 (1) ◽

pp. 53 ◽

Cited By ~ 3

Author(s):

K. DÓZSA-FARKAS ◽

G. CECH

Keyword(s):

New Species ◽

Distinct Species ◽

The Body ◽

Molecular Methods ◽

Similar Species ◽

Pcr Rflp ◽

Molecular Comparison ◽

Type C ◽

Epidermal Gland

A new species, Fridericia crassiductata sp.n. was described and compared by DNA fragment analyses (PCR-RFLP), with two similar species, Fridericia ratzeli (Eisen,1872) sensu Nielsen & Christensen (1959) and F. eiseni Dózsa-Farkas, 2005. The main characteristics of the new species are: 13–20 mm long, 0.5–0.7 mm wide, segment number: (38)–40–56, spermatheca with 9–10 large, sessile, globular diverticula, long and thick ectal duct, and two very large (80–130 µ m long) egg shaped ectal glands. Maximum ten chaetae per bundle, typical brown reticulate epidermal gland cells noticeable on the body surface. The oesophageal appendage is variable between type-a and type-c (according to Möller 1971) the branches are located proximally. The seminal vesicle is large, the penial slit is longitudinal with more transverse components. Three subneural glands in XIV–XVI. The new species was collected only in the Zemplén Mountains in Hungary, in similar biotopes (between and under the leaf-litter) to those of the common F. ratzeli. It may be hypothesized that it fills the ecological role of F. ratzeli because, although all three compared species occurred in this area, F. ratzeli was very seldom found and never together with the new species. The three species were also examined by molecular methods. Which confirmed the existence of three distinct species. Both morphological and molecular studies indicate that F. ratzeli is still not a homogeneous species. It seems that the applied molecular methods help distinguish morphologically very similar species.

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Simultaneous identification of molecular and mating types within the Cryptococcus species complex by PCR-RFLP analysis

Journal of Medical Microbiology ◽

10.1099/jmm.0.2008/003665-0 ◽

2008 ◽

Vol 57 (12) ◽

pp. 1481-1490 ◽

Cited By ~ 26

Author(s):

Xiaobo Feng ◽

Zhirong Yao ◽

Daming Ren ◽

Wanqing Liao

Keyword(s):

Species Complex ◽

Rflp Analysis ◽

Mating Types ◽

Environmental Isolates ◽

Serotype A ◽

Pcr Rflp ◽

Cryptococcus Species ◽

Systemic Infections ◽

Simultaneous Identification ◽

Ad Hybrids

The Cryptococcus species complex consists of two species, Cryptococcus neoformans and Cryptococcus gattii, which cause systemic infections in both immunocompromised and immunocompetent patients. Both species have a bipolar mating system, with mating type (MAT) α being predominant in clinical and environmental isolates. The strains of the Cryptococcus species complex have been divided into eight major molecular types, which show differences in epidemiology, biology and pathogenicity. In this study, two PCR-RFLP analyses, based on the CAP1 and GEF1 genes, which are both located at the MAT locus, were developed for simultaneous identification of the molecular and mating types of isolates of the Cryptococcus species complex. The molecular and mating types of all 144 cryptococcal isolates, including rare subtypes, were successfully determined by both PCR-RFLP approaches. Pattern analysis of the AD hybrids revealed that the serotype A MAT a allele in strains of AaDα derived from genotype VNB, whereas the serotype A MATα allele among strains of AαDa and AαDα derived from molecular type VNI.

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Indoor collections of the Anopheles funestus group (Diptera: Culicidae) in sprayed houses in northern KwaZulu-Natal, South Africa

Malaria Journal ◽

10.1186/1475-2875-6-30 ◽

2007 ◽

Vol 6 (1) ◽

pp. 30 ◽

Cited By ~ 24

Author(s):

Joel C Mouatcho ◽

Keith Hargreaves ◽

Lizette L Koekemoer ◽

Basil D Brooke ◽

Shüne V Oliver ◽

...

Keyword(s):

South Africa ◽

Anopheles Funestus ◽

Kwazulu Natal ◽

Anopheles Funestus Group

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Simultaneous identification of Bulbus Fritillariae cirrhosae using PCR-RFLP analysis

Phytomedicine ◽

10.1016/j.phymed.2006.09.008 ◽

2007 ◽

Vol 14 (9) ◽

pp. 628-632 ◽

Cited By ~ 30

Author(s):

C.Z. Wang ◽

P. Li ◽

J.Y. Ding ◽

X. Peng ◽

C.S. Yuan

Keyword(s):

Rflp Analysis ◽

Pcr Rflp ◽

Simultaneous Identification

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Hpall endonuclease distinguishes between two species in the Anopheles funestus group

Insect Molecular Biology ◽

10.1046/j.1365-2583.1998.00072.x ◽

1998 ◽

Vol 7 (3) ◽

pp. 273-277 ◽

Cited By ~ 14

Author(s):

L. L. Koekemoer ◽

M. Coetzee ◽

R. H. Hunt

Keyword(s):

Anopheles Funestus ◽

Anopheles Funestus Group

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Detection of F94L mutation of the MSTN gene in four Indonesian local cattle breeds

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.45.1.7-14 ◽

2020 ◽

Vol 45 (1) ◽

pp. 7-14

Author(s):

S. Anwar ◽

S. D. Volkandari ◽

A. S. Wulandari ◽

W. P. B. Putra ◽

E. Sophian ◽

...

Keyword(s):

Meat Quality ◽

Wild Type ◽

Myostatin Gene ◽

Mstn Gene ◽

Cattle Breeds ◽

Holstein Friesian ◽

Pcr Rflp ◽

Genotype Identification ◽

The Status ◽

Type C

The F94L mutation of the MSTN gene (MSTN-F94L) is considered not to cause disrupted the function of the myostatin gene drastically. Interestingly, this mutation has a very significant effect on muscle mass, carcass, or meat yield and meat quality without any associated severe negative problems. This study aimed to confirm the MSTN-F94L mutation in four local cattle breeds in Indonesia. A total of 518 individuals (140 of Bali, 107 of Sumbawa, 168 of Pasundan, and 103 of Holstein-Friesian (H-F) cattle) were used in this study. Genotype identification was performed by PCR-RFLP method. In the present study showed that the wild-type C allele was fixed (1.000) in Bali, Sumbawa, and HF cattle. However, the wild-type C allele and the mutant A allele were found in Pasundan cattle, even though the frequency of the mutant A allele was very low (0.012). Therefore, in conclusion, the mutation of the MSTN-F94L was detected in Pasundan cattle but no in all three cattle breeds. However, the presence of the mutant A allele in Pasundan cattle allegedly derived from Limousin bulls. The further investigation in other local and exotic breeds and its crossing will answer the status of the MSTN-F94L mutation in local cattle breeds in Indonesia.

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