scholarly journals A Comparative Examination of Swine Sera for Antibody To Aujeszky Virus with the Conventional and a Modified Virus-Serum Neutralization Test and a Modified Direct Complement Fixation Test

1976 ◽  
Vol 17 (2) ◽  
pp. 142-152
Author(s):  
V. Bitsch ◽  
M. Eskildsen
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Neutralization Test ◽  
Fixation Test ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Comparative Examination

scholarly journals Direct complement fixation test with avian infectious bronchitis virus in chickens

1970 ◽  
Vol 68 (2) ◽  
pp. 211-219 ◽  
Author(s):  
P. K. Uppal
Keyword(s):  
Infectious Bronchitis Virus ◽  
Neutralizing Antibodies ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Allantoic Fluid ◽  
Fixation Test ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Primary Inoculation ◽  
Serum Neutralization Test

SUMMARYThe direct complement fixation test was performed to follow the antibody response in chickens infected with avian infectious bronchitis virus. Concentrated allantoic fluid (4 units) was used as an antigen and allowed to react with serially diluted antiserum in the presence of two complete units of guinea-pig complement for 3 hr. at 4°C. and ½ hr. at 37°C. before the addition of sensitized cells. Serum was unheated and used either fresh or within one month of storage at −30°C. Individual birds showed a rise and fall of complement-fixing antibody both after primary and secondary inoculations. The complement-fixing antibody was detected as early as the seventh day after primary inoculation. The highest complement fixation titre (1/32 to 1/64) was recorded from 14 to 21 days after inoculation with a subsequent gradual decline.The results of the direct complement fixation tests have been correlated with the serum neutralization test. The neutralizing antibodies usually appeared by the 14th day but were not detected at a significant titre until the 21st day after primary inoculation. Serum neutralizing antibodies were still present at high titres even after 7 weeks of infection but the complement-fixing antibodies had disappeared by that time.

scholarly journals Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains

1978 ◽  
Vol 81 (1) ◽  
pp. 107-123 ◽  
Author(s):  
M. M. Rweyemamu ◽  
J. C. Booth ◽  
Morwen Head ◽  
T. W. F. Pay
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Neutralization Test ◽  
Foot And Mouth Disease ◽  
Fixation Test ◽  
Viral Contamination ◽  
Serological Typing ◽  
Mouth Disease Virus ◽  
Virus Strains ◽  
Microneutralization Test

SUMMARYA microneutralization test for serotyping of FMD viruses is described. It is based on earlier observations by Booth, Rweyemamu & Pay (1978) that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90% of samples a positive typing was obtained in contrast with about 50% for the complement fixation test. The test was also found to be susceptible to minimal quantities of heterotypic viral contamination.For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was found to be more specific. The necessity to utilize virus-neutralization test systems for comparing (FMD) virus strains particularly for the purpose of vaccine selection is emphasized. The two dimensional microneutralization test has been applied to a study of comparing FMDV vaccine strains for Europe, South America, the Middle East and East Africa.

Mumps Antibody: Placental Transfer and Disappearance During the First Year of Life

PEDIATRICS ◽  
1970 ◽  
Vol 45 (1) ◽  
pp. 99-101
Author(s):  
David Hodes ◽  
Philip A. Brunell
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Placental Transfer ◽  
Neutralization Test ◽  
Serum Antibody ◽  
Neutralizing Antibody ◽  
Fixation Test ◽  
First Year ◽  
First Year Of Life

Mumps neutralizing antibody was transferred quantitatively across the placenta. Antibody was still detectable (≥ 1:2) in 18 of 19 infants at 2 months of age. The finding of antibody in 13 of 19 infants at 5 months of age probably accounted for the failure to immunize infants at this age successfully in previous studies. Neutralizing antibody was not detectable sera of any of the 18 infants who were tested at 1 year of age. Although serum antibody during the early months of life was presumably all IgG since it was passively acquired, the neutralization test appeared to be far more sensitive than the complement fixation test for the determination of mumps antibody.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Assessment of a complement fixation test to detect Mycoplasma hyopneumoniae infection in pigs

1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycoplasma Hyopneumoniae ◽  
Fixation Test

Effect of Calcium Ion on the Kolmer Complement-Fixation Test

1954 ◽  
Vol 24 (8) ◽  
pp. 934-945 ◽  
Author(s):  
Alcor S. Browne ◽  
Martha M. Michelbacher ◽  
Edith M. Coffey
Keyword(s):  
Calcium Ion ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

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