scholarly journals Fine mapping of two grain chalkiness QTLs sensitive to high temperature in rice

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Weifeng Yang ◽  
Jiayan Liang ◽  
Qingwen Hao ◽  
Xin Luan ◽  
Quanya Tan ◽  
...  
Keyword(s):  
High Temperature ◽  
Chromosome 9 ◽  
Rice Grain ◽  
Chromosome 11 ◽  
Substitution Lines ◽  
Single Segment Substitution Lines ◽  
Cropping Season ◽  
Cropping Seasons ◽  
Segment Substitution ◽  
Grain Chalkiness

Abstract Background Grain chalkiness is one of important factors affected rice grain quality. It is known that chalkiness is affected by the high temperature during the seed filling period. Although a larger of QTLs for chalkiness were reported across all 12 chromosomes, only a few of the QTLs were fine mapped or cloned up to now. Here, we fine map two QTLs for chalkiness in two single-segment substitution lines (SSSLs), 11–09 with substitution segment from O. sativa and HP67–11 with substitution segment from O. glaberrima. Results The grain chalkiness of SSSLs 11–09 and HP67–11 was significantly lower than that in the recipient Huajingxian 74 (HJX74) in consecutive 8 cropping seasons. The regression correlation analysis showed that percentage of chalky grain (PCG) and percentage of chalky area (PCA) were significantly and positively correlated with percentage of grain chalkiness (PGC). Two QTLs for grain chalkiness were located on two chromosomes by substitution mapping. qPGC9 was mapped on chromosome 9 with an estimated interval of 345.6 kb. qPGC11 was located on chromosome 11 and delimited to a 432.1 kb interval in the O. sativa genome and a 332.9 kb interval in the O. glaberrima genome. qPGC11 is a QTL for grain chalkiness from O. glaberrima and was mapped in a new region of chromosome 11. The effect of two QTLs was incomplete dominance. The additive effects of two QTLs on chalkiness in second cropping season (SCS) were significantly greater than that in first cropping season (FCS). Conclusions qPGC11 is a new QTL for grain chalkiness. The two QTLs were fine mapped. The donor alleles of qPGC9 and qPGC11 were sensitive to the high temperature of FCS.

QTL Detection for Rice Grain Shape Using Chromosome Single Segment Substitution Lines

Rice Science ◽  
2011 ◽  
Vol 18 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Sheng-qiang LI ◽  
Guo-kun CUI ◽  
Cheng-ran GUAN ◽  
Jun WANG ◽  
Guo-hua LIANG
Keyword(s):  
Grain Shape ◽  
Rice Grain ◽  
Qtl Detection ◽  
Substitution Lines ◽  
Single Segment ◽  
Single Segment Substitution Lines ◽  
Segment Substitution

Mapping of QTLs for Grain Shape Using Chromosome Single Segment Substitution Lines in Rice (Oryza sativaL.)

2013 ◽  
Vol 39 (4) ◽  
pp. 617
Author(s):  
Jun WANG ◽  
Jin-Yan ZHU ◽  
Yong ZHOU ◽  
Jie YANG ◽  
Fang-Jun FAN ◽  
...  
Keyword(s):  
Grain Shape ◽  
Substitution Lines ◽  
Single Segment ◽  
Single Segment Substitution Lines ◽  
Segment Substitution

Dynamic expression of nine QTLs for tiller number detected with single segment substitution lines in rice

2008 ◽  
Vol 118 (3) ◽  
pp. 443-453 ◽  
Author(s):  
Guifu Liu ◽  
Ruizhen Zeng ◽  
Haitao Zhu ◽  
Zemin Zhang ◽  
Xiaohua Ding ◽  
...  
Keyword(s):  
Tiller Number ◽  
Substitution Lines ◽  
Single Segment ◽  
Single Segment Substitution Lines ◽  
Dynamic Expression ◽  
Segment Substitution

Identification, Pyramid and Candidate gene of QTL for Yield-related Traits Based on Rice CSSLs in Indica Xihui18 Background

2021 ◽  
Author(s):  
Shuangfei Sun ◽  
Siqian Xiang ◽  
Meng Lv ◽  
Kai Zhou ◽  
Juan Li ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Candidate Genes ◽  
Target Genes ◽  
Molecular Design ◽  
Mechanism Analysis ◽  
Substitution Lines ◽  
Cell Width ◽  
Analysis And Design ◽  
Single Segment Substitution Lines ◽  
Segment Substitution

Abstract Chromosome segment substitution line (CSSL) in rice is important for functional analysis and design breeding of target genes. Here, a novel rice CSSL-Z431 was identified from indica restorer line Xihui18 as recipient and Huhan3 as donor. Z431 contained six segments from Huhan3, with an average substitution length of 2.12 Mb. Compared with Xihui18, Z431 increased panicles per plant (PN) and displayed short-wide grains. The short-wide grain of Z431 were caused by reducing of cell length and increasing of cell width in the glume. Then, thirteen QTLs were identified in a secondary F2 population derived from Xihui18/Z431. Among them, six QTLs (qPN3, qGL3, qGW5, qRLW2, qRLW3, qGWT-5-2) were validated by four single-segment substitution lines (SSSLs, S1-S4) developed in F3. In addition, thirteen QTLs (qPN1, qPN2, qPL1, qPL2, qGPP1, qGPP2, qGL2, qGW1, qGW2, qGW3, qRLW5-2, qRLW1, qGWT2) were detected by these SSSLs, while not be identified in the F2 population from Xihui18/Z431. Increase of panicles per plant in Z431 was controlled by qPN3, qPN1 and qPN2. OsIAGLU should be the candidate gene for qPN3 by DNA sequencing. The short-wide grain of Z431 was controlled by qGL3, qGL2, qGW5, qGW2 and qGW3. By sequencing between Xihui18 and according SSSL, three candidate genes for qGL3 and two candidate genes for qGW5 were identified, respectively. In addition, pyramid of different QTLs (qPN1and qPN3; qPN2 and qPN3 etc.) yielded different epistatic effects. These results lay good foundation in molecular mechanism analysis of unreported genes and rice molecular design breeding.

Sign in / Sign up

Export Citation Format

Share Document