scholarly journals Correction to: Genome wide effects of oleic acid on cultured bovine granulosa cells: evidence for the activation of pathways favoring folliculo-luteal transition

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Vengala Rao Yenuganti ◽  
Dirk Koczan ◽  
Jens Vanselow
Keyword(s):  
Oleic Acid ◽  
Granulosa Cells ◽  
Genome Wide

scholarly journals Genome wide effects of oleic acid on cultured bovine granulosa cells: evidence for the activation of pathways favoring folliculo-luteal transition

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Vengala Rao Yenuganti ◽  
Dirk Koczan ◽  
Jens Vanselow
Keyword(s):  
Oleic Acid ◽  
Granulosa Cells ◽  
System Development ◽  
Circulatory System ◽  
Bioinformatic Analysis ◽  
Negative Energy ◽  
Tissue Remodelling ◽  
Gonadotropin Secretion ◽  
Genome Wide

Abstract Background Metabolic stress, as negative energy balance on one hand or obesity on the other hand can lead to increased levels of free fatty acids in the plasma and follicular fluid of animals and humans. In an earlier study, we showed that increased oleic acid (OA) concentrations affected the function of cultured bovine granulosa cells (GCs). Here, we focus on genome wide effects of increased OA concentrations. Results Our data showed that 413 genes were affected, of which 197 were down- and 216 up-regulated. Specifically, the expression of FSH-regulated functional key genes, CCND2, LHCGR, INHA and CYP19A1 and 17-β-estradiol (E2) production were reduced by OA treatment, whereas the expression of the fatty acid transporter CD36 was increased and the morphology of the cells was changed due to lipid droplet accumulation. Bioinformatic analysis revealed that associated pathways of the putative upstream regulators “FSH” and “Cg (choriogonadotropin)” were inhibited and activated, respectively. Down-regulated genes are over-represented in GO terms “reproductive structure/system development”, “ovulation cycle process”, and “(positive) regulation of gonadotropin secretion”, whereas up-regulated genes are involved in “circulatory system development”, “vasculature development”, “angiogenesis” or “extracellular matrix/structure organization”. Conclusions From these data we conclude that besides inhibiting GC functionality, increased OA levels seemingly promote angiogenesis and tissue remodelling, thus suggestively initiating a premature fulliculo-luteal transition. In vivo this may lead to impeded folliculogenesis and ovulation, and cause sub-fertility.

Pool-based genome-wide association study identified novel candidate regions on BTA9 and 14 for oleic acid percentage in Japanese Black cattle

2018 ◽  
Vol 89 (8) ◽  
pp. 1060-1066 ◽  
Author(s):  
Fuki Kawaguchi ◽  
Hiroto Kigoshi ◽  
Ayaka Nakajima ◽  
Yuta Matsumoto ◽  
Yoshinobu Uemoto ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Association Study ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Japanese Black Cattle ◽  
Genome Wide ◽  
Candidate Regions ◽  
Acid Percentage

Integrated analysis of transcriptome and histone modifications in granulosa cells during ovulation in female mice

Endocrinology ◽  
2021 ◽  
Author(s):  
Yuichiro Shirafuta ◽  
Isao Tamura ◽  
Yasuyuki Ohkawa ◽  
Ryo Maekawa ◽  
Yumiko Doi-Tanaka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune System ◽  
Inflammatory Response ◽  
Histone Modifications ◽  
Granulosa Cells ◽  
Cellular Functions ◽  
Lh Surge ◽  
Genome Wide ◽  
Ros Metabolism ◽  
Rapid Changes

Abstract The ovulatory luteinizing hormone (LH) surge induces rapid changes of gene expression and cellular functions in granulosa cells (GCs) undergoing luteinization. However, it remains unclear how the changes in genome-wide gene expression are regulated. H3K4me3 histone modifications are involved in rapid alteration of gene expression. In this study, we investigated genome-wide changes of transcriptome and H3K4me3 status in mouse GCs undergoing luteinization. GCs were obtained from mice treated with equine chorionic gonadotropin (eCG) before, 4 h and 12 h after human (h)CG injection. RNA-sequencing identified a number of up- and down-regulated genes, which could be classified into eight patterns according to the time-course changes of gene expression. Many genes were transiently up- or down-regulated at 4 h after hCG stimulation. Gene ontology terms associated with these genes included steroidogenesis, ovulation, COC expansion, angiogenesis, immune system, ROS metabolism, inflammatory response, metabolism and autophagy. The cellular functions of DNA repair and cell growth were newly identified as being activated during ovulation. ChIP-sequencing revealed a genome-wide and rapid change of H3K4me3 during ovulation. Integration of transcriptome and H3K4me3 data identified many H3K4me3-associated genes that are involved in steroidogenesis, ovulation, COC expansion, angiogenesis, inflammatory response, immune system, ROS metabolism, lipid and glucose metabolism, autophagy, and regulation of cell size. The present results suggest that genome-wide changes in H3K4me3 after the LH surge are associated with rapid changes in gene expression in GCs, which enables GCs acquire a lot of cellular functions within a short time that are required for ovulation and luteinization.

scholarly journals Multiomics Analysis Reveals Molecular Abnormalities in Granulosa Cells of Women With Polycystic Ovary Syndrome

2021 ◽  
Vol 12 ◽  
Author(s):  
Rusong Zhao ◽  
Yonghui Jiang ◽  
Shigang Zhao ◽  
Han Zhao
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Granulosa Cells ◽  
Polycystic Ovary ◽  
Reproductive Age ◽  
Differentially Expressed ◽  
Steroid Biosynthesis ◽  
Expression Array ◽  
Ovary Syndrome ◽  
Molecular Abnormalities ◽  
Genome Wide

Polycystic ovary syndrome (PCOS) is the most common complex endocrine and metabolic disease in women of reproductive age. It is characterized by anovulatory infertility, hormone disorders, and polycystic ovarian morphology. Regarding the importance of granulosa cells (GCs) in the pathogenesis of PCOS, few studies have investigated the etiology at a single “omics” level, such as with an mRNA expression array or methylation profiling assay, but this can provide only limited insights into the biological mechanisms. Here, genome-wide DNA methylation together with lncRNA-miRNA-mRNA profiles were simultaneously detected in GCs of PCOS cases and controls. A total of 3579 lncRNAs, 49 miRNAs, 669 mRNAs, and 890 differentially methylated regions (DMR)-associated genes were differentially expressed between PCOS cases and controls. Pathway analysis indicated that these differentially expressed genes were commonly associated with steroid biosynthesis and metabolism-related signaling, such as glycolysis/gluconeogenesis. In addition, we constructed ceRNA networks and identified some known ceRNA axes, such as lncRNAs-miR-628-5p-CYP11A1/HSD17B7. We also identified many new ceRNA axes, such as lncRNAs-miR-483-5p-GOT2. Interestingly, most ceRNA axes were also closely related to steroid biosynthesis and metabolic pathways. These findings suggest that it is important to systematically consider the role of reproductive and metabolic genes in the pathogenesis of PCOS.

scholarly journals GWAS and co-expression network combination uncovers multigenes with close linkage effects on oleic acid content accumulation in Brassica napus

2020 ◽  
Author(s):  
Min Yao ◽  
Mei Guan ◽  
Zhenqian Zhang ◽  
Qiuping Zhang ◽  
Yixin Cui ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Candidate Genes ◽  
Acid Content ◽  
Complex Traits ◽  
Snp Array ◽  
Oleic Acid Content ◽  
Crop Species ◽  
Genome Wide ◽  
A Genome ◽  
Chromosome Haplotype

Abstract Background: Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes. GWAS combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species. Results: We use the 60K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with oleic acid (C18:1) in rapeseed. Six haplotype regions were identified as significantly associated with oleic acid (C18:1) that mapped to chromosomes A02, A07, A08, C01, C02, and C03. Additionally, whole-genome sequencing of 50 rapeseed accessions revealed three genes ( BnmtACP2-A02 , BnABCI13-A02 and BnECI1-A02 ) in the A02 chromosome haplotype region and two genes ( BnFAD8-C02 and BnSDP1-C02 ) in the C02 chromosome haplotype region that were closely linked to oleic acid content phenotypic variation. Moreover, the co-expression network analysis uncovered candidate genes from these two different haplotype regions with potential regulatory interrelationships with oleic acid content accumulation. Conclusions: Our results suggest that several candidate genes are closely linked, which provides us with an opportunity to develop functional haplotype markers for the improvement of the oleic acid content in rapeseed.

scholarly journals GWAS and co-expression network combination uncovers multigenes with close linkage effects on oleic acid content accumulation in Brassica napus

2020 ◽  
Author(s):  
Lunwen Qian ◽  
Min Yao ◽  
Mei Guan ◽  
Zhenqian Zhang ◽  
Yixin Cui ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Candidate Genes ◽  
Acid Content ◽  
Complex Traits ◽  
Snp Array ◽  
Oleic Acid Content ◽  
Crop Species ◽  
Genome Wide ◽  
A Genome ◽  
Chromosome Haplotype

Abstract Background: Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes. GWAS combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species. Results: We use the 60K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with oleic acid (C18:1) in rapeseed. Six haplotype regions were identified as significantly associated with oleic acid (C18:1) that mapped to chromosomes A02, A07, A08, C01, C02, and C03. Additionally, whole-genome sequencing of 50 rapeseed accessions revealed three genes ( BnmtACP2-A02 , BnABCI13-A02 and BnECI1-A02 ) in the A02 chromosome haplotype region and two genes ( BnFAD8-C02 and BnSDP1-C02 ) in the C02 chromosome haplotype region that were closely linked to oleic acid content phenotypic variation. Moreover, the co-expression network analysis uncovered candidate genes from these two different haplotype regions with potential regulatory interrelationships with oleic acid content accumulation. Conclusions: Our results suggest that several candidate genes are closely linked, which provides us with an opportunity to develop functional haplotype markers for the improvement of the oleic acid content in rapeseed.

P–596 Association of oleic acid production in cumulus-granulosa cells with glutathione of in vitro matured oocytes

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Fayezi ◽  
M Ghaffar. Novin ◽  
M Norouzian ◽  
M Nouri ◽  
L Farzadi
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Oocyte Maturation ◽  
Granulosa Cells ◽  
Acid Production ◽  
In Vitro Maturation ◽  
Acid Synthesis ◽  
Glutathione Level ◽  
Metaphase Ii Oocytes

Abstract Study question Does oleic acid production in cumulus-granulosa cells affect glutathione levels of in vitro matured oocytes? Summary answer Oleic acid availability in cumulus-granulosa cells is associated with a higher glutathione level in in vitro matured oocytes. What is known already The monounsaturated fatty acid oleic acid is de novo synthesized by desaturation of stearic acid and can promote steroidogenesis and oocyte development in vitro. The endogenous antioxidant glutathione content in metaphase II oocyte is significantly higher than immature stages and is related to the normal oocyte maturation. Study design, size, duration Mouse germinal vesicles were co-cultured for 24 hours, during in vitro maturation, with granulosa cells treated with a specific inhibitor of oleic acid synthesis alone or in combination with oleic acid. Participants/materials, setting, methods Fluorescence staining was used to assess the glutathione content of mouse metaphase II oocytes following in vitro maturation as an indicator of cytoplasmic maturation. Glutathione was stained using Cell Tracker Blue –CMAC for 30 min at 37 °C. After being washed in fresh media, stained oocytes were photographed by a fluorescence microscope. Cell area and associated fluorescence were quantified in 20 metaphase II mouse oocytes randomly chosen from in vitro matured oocytes for each condition. Main results and the role of chance The intracellular glutathione content was profoundly lower in metaphase II oocytes obtained from co-cultures with inhibitor-treated cumulus-granulosa cells than with the control cumulus cells (–50%, p < 0.01). Oleic acid effectively recovered the negative effect of inhibitor on glutathione level nearly up to the level of the mock-treated cells. Limitations, reasons for caution The findings are limited to metaphase II. Measurement at more advanced stages of oocyte development is of interest. Inhibition of cellular fatty acid synthesis was performed solely with a specific chemical. Wider implications of the findings: Involvement of the oleic acid availability for cumulus-granulosa cells in normal oocyte maturation may be of relevance in reproductive disorders, particularly in the pathological mechanism of impaired oogenesis. Trial registration number 400/3226

scholarly journals GWAS and co-expression network combination uncovers multigenes with close linkage effects on oleic acid content accumulation in Brassica napus

2019 ◽  
Author(s):  
Lunwen Qian ◽  
Min Yao ◽  
Mei Guan ◽  
Zhenqian Zhang ◽  
Qiuping Zhang ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Candidate Genes ◽  
Acid Content ◽  
Complex Traits ◽  
Snp Array ◽  
Oleic Acid Content ◽  
Crop Species ◽  
Genome Wide ◽  
A Genome ◽  
Chromosome Haplotype

Abstract Abstract Background: Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes. GWAS in combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species. Results: We used the 60K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with oleic acid (C18:1) in rapeseed. Six haplotype regions were identified as significantly associated with oleic acid (C18:1) that mapped to chromosomes A02, A07, A08, C01, C02 and C03. Additionally, whole-genome sequencing of 50 rapeseed lines revealed three genes (BnmtACP2-A02, BnABCI13-A02 and BnECI1-A02) in the A02 chromosome haplotype region and two genes (BnFAD8-C02 and BnSDP1-C02) in the C02 chromosome haplotype region that were closely linked to oleic acid content phenotypic variation. Moreover, the co-expression network analysis uncovered candidate genes from these two different haplotype regions with potential regulatory interrelationships with oleic acid content accumulation. Conclusions: Our results suggest that several candidate genes are closely linked, which provides us with an opportunity to develop functional haplotype markers for the improvement of the oleic acid content in rapeseed.

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