Schistosomicidal, hepatoprotective and antioxidant activities of the methanolic fraction from Clerodendrum umbellatum Poir leaves aqueous extract in Schistosoma mansoni infection in mice

Background: One of the four most incident species in mangrove is the Laguncularia racemosa, plant widely used in popular medicine against inflammation and fever. Objective: Here, L. racemosa was investigated in relation to their phytochemical profile, antioxidant activity, citotoxicity, antimicrobial and immunostimulatory effect. Method: Aqueous extract was obtained from leaves of plant, its phytochemical profile was investigated through UPLC method, the antioxidant assays performed were TAA, DPPH, ABTS, nitrite and lipid peroxidation assay. Antimicrobial assays were made using standard strains. For all biological tests were used mice splenocytes and from these cell cultures were measured cytotoxicity, proliferation index and cytokines production. Results: Laguncularia racemosa leaves showed the presence of ions, proteins, carbohydrates, vitamins and high concentration of phenolic compounds. Antioxidant activities were promoted by aqueous extract, especially in DPPH and NO assays. Extract in 6 µg/mL did not induce significant cell death, stimulated the cell proliferation and the IL-4 production. Moreover, decreases of proinflammatory cytokines IFN-γ, TNF-α and IL-6 were found. Conclusion: The presence of essential nutrients, significant antioxidant activity and immune stimulation confirm the use of this plant in folk medicine against inflammation.

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Antioxidant Evaluation of the Aqueous Extract of Hulls of Campsiandra laurifolia in Colitis Induced by Acetic Acid in Wistar Rats

Journal of Coloproctology ◽

10.1055/s-0041-1730258 ◽

2021 ◽

Vol 41 (02) ◽

pp. 138-144

Author(s):

Lucas Petitemberte de Souza ◽

Henrique Sarubbi Fillmann ◽

Sandielly Rebeca Benitez da Fonseca ◽

Marilda da Silva Brasil ◽

Norma Anair Possa Marroni ◽

...

Keyword(s):

Acetic Acid ◽

Phenolic Compounds ◽

Aqueous Extract ◽

Wistar Rats ◽

Antioxidant Activities ◽

Experimental Colitis ◽

Anal Pressure ◽

Antioxidant Power ◽

Significance Level ◽

Sphincter Anal

AbstractDue to the ethnopharmacological use of Campsiandra laurifolia (Fabaceae), popularly known as Acapurana, to treat wounds and ulcers, associated with the lack of alternative treatments for intestinal inflammations such as ulcerative colitis (UC), the present work sought to characterize its phytochemical and antioxidant activities, and to evaluate remedial action in experimental colitis with acetic acid. Phytochemical analyzes were performed through qualitative and quantitative colorimetric tests of the main secondary metabolites. In the colitis model, 24 male Wistar rats aged ± 60 days old were used, divided into 4 groups: Control (CO) control + aqueous extract of C. laurifolia 50 mg/kg (CO + A50); Colitis (CL); and Colitis + aqueous extract of C. laurifolia 50 mg/kg (CL + A50). Measurement of sphincter anal pressure and histological tests of the large intestine, lipoperoxidation (LPO), enzyme activity of superoxide dismutase (SOD), and levels of glutathione (GSH) were performed. For statistical analysis, the oxidative stress (OS) results were expressed as means ± standard error, adopting a significance level of p < 0.05. The screening indicated the presence of flavonoids, saponins and tannins in the extract, with high levels of phenolic compounds and tannins, and was related to high antioxidant capacity. In the histological analysis, the CL group presented loss of the crypts, edema and inflammatory infiltrate. The use of C. laurifolia extract restructured the crypts, decreased edema and increased sphincter anal pressure, with a decrease in LPO, SOD, and an increase in GSH. It is suggested that the use of C. laurifolia extract reduces OS due to its antioxidant power conferred by the phenolic compounds present in the extract.

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Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Molecules ◽

10.3390/molecules26113303 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3303

Author(s):

Amina M. G. Zedan ◽

Mohamed I. Sakran ◽

Omar Bahattab ◽

Yousef M. Hawsawi ◽

Osama Al-Amer ◽

...

Keyword(s):

Phenolic Compounds ◽

Aqueous Extract ◽

Antioxidant Activities ◽

Individual Treatment ◽

Alcoholic Extract ◽

Anticancer Effect ◽

Mcf7 Cells ◽

Vespa Orientalis ◽

Anti Inflammatory

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.

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Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

Phytothérapie ◽

10.3166/phyto-2021-0282 ◽

2021 ◽

Author(s):

E.K. Soltani ◽

K. Zaim ◽

K. Mokhnache ◽

N. Haichour ◽

S. Mezaache-Aichour ◽

...

Keyword(s):

Aqueous Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antibacterial Properties ◽

Natural Antioxidants ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Biological Properties ◽

Aqueous Extracts

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

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Influence of Drying Methods on Antioxidant Activities and Immunomodulatory of Aqueous Extract From Soybean Curd Residue Fermentated by Grifola frondosa

International Journal of Biology ◽

10.5539/ijb.v7n1p82 ◽

2014 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Dan Zhu ◽

Hongyi Sun ◽

Shuhong Li ◽

Xuansheng Hu ◽

Xi Yuan ◽

...

Keyword(s):

Aqueous Extract ◽

Antioxidant Activities ◽

Grifola Frondosa ◽

Drying Methods ◽

Soybean Curd ◽

Soybean Curd Residue

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In vitro antioxidant and antidiarrheal activities of aqueous and n-hexane extracts of Cucurbita maxima seed in castor oil-induced diarrheal rats

10.53365/nrfhh/144728 ◽

2022 ◽

Author(s):

Habibu Tijjani ◽

Adamu Matinja ◽

Marwanatu Yahya ◽

Emmanuel Aondofa ◽

Akibu Sani

Keyword(s):

Vitamin C ◽

Aqueous Extract ◽

Castor Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Seed Extract ◽

Hexane Extract ◽

Cucurbita Maxima ◽

Seed Extracts

Diarrhea is a common health complaint occurring with mild, temporary conditions to a potentially life-threatening condition. Cucurbita maxima (Cucurbitaceae) seed is reportedly used traditionally for the treatment of diarrheal and thus this study aimed to evaluate the in vitro antioxidants, total tannins, phenolics contents, and antidiarrheal potentials of n-hexane and aqueous C. maxima seed extracts in castor oil induced diarrheal rats. The n-hexane extract mainly contains oils while the aqueous extract was thick, brown solid. The aqueous and n-hexane seed extract of C. maxima expressed significant 2,2, diphenyl-1-picrythdrazyl (DPPH) scavenging activities at 6.25 – 1000 mg/mL with IC50 values of 104.01 mg/mL, 29.27 mg/mL and 26.78 mg/mL for n-hexane, aqueous and vitamin C respectively. The hydroxyl radical scavenging activities of the n-hexane and aqueous seed extract of C. maxima were significantly lower at higher concentrations compared with that of vitamin C. Furthermore, the total antioxidant capacities of n-hexane (22.08mg/mL) and aqueous seed extract (11.03 mg/mL) of C. maxima were found to be higher than that of vitamin C (134.46 mg/mL). The aqueous extracts (658.33±380.08 mg QE/g) total tannins were not significantly different from the n-hexane extract (468.33±102.55 mg QE/g) while the n-hexane extract contains significantly higher total phenolics (2.93±1.25 mg GAE/g) compared with the aqueous extract (0.19±0.04 mg GAE/g). Percentage inhibition of stooling was found to be 57±22%, 41±16%, and 46±11% for loperamide, n-hexane, and aqueous respectively. The study concludes that aqueous and n-hexane seed extracts of C. maxima (pumpkin) possess in vitro antioxidant activities and antidiarrheal properties.

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Morphological changes in the pancreas and glucose reduction of the aqueous extract of Costus afer leaf on alloxan-induced diabetic rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2014-0033 ◽

2015 ◽

Vol 26 (6) ◽

Cited By ~ 4

Author(s):

Anthoneth Ndidi Ezejiofor ◽

Chinna Nneka Orish ◽

Orish Ebere Orisakwe

Keyword(s):

Aqueous Extract ◽

Antioxidant Activities ◽

Morphological Changes ◽

Study Groups ◽

Diabetic Rats ◽

Albino Rats ◽

Protective Properties ◽

Glucose Reduction ◽

Different Doses ◽

Costus Afer

AbstractConsidering the antihyperglycemic and antioxidant activities of herbs, this study has evaluated the morphological changes, hypoglycemic effect, and comparative ameliorating effects ofThirty adult male albino rats that were divided into six groups of five weight-matched animals each were used in the study. Groups 1 and 2 served as controls, whereas groups 3–6 were alloxan-induced diabetic groups treated with different doses of the extract (375, 750, and 1125 mg/kgThe phytoconstituents ofleaves possess both antidiabetic and tissue protective properties on pancreases of investigated rats.

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The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

Antioxidants ◽

10.3390/antiox9040304 ◽

2020 ◽

Vol 9 (4) ◽

pp. 304 ◽

Cited By ~ 1

Author(s):

Sara Abidar ◽

Razvan Boiangiu ◽

Gabriela Dumitru ◽

Elena Todirascu-Ciornea ◽

Amina Amakran ◽

...

Keyword(s):

Aqueous Extract ◽

High Performance ◽

Antioxidant Activities ◽

Memory Performance ◽

Underlying Mechanism ◽

Ceratonia Siliqua ◽

Ceratonia Siliqua L ◽

6 Hydroxydopamine

Ceratonia siliqua L. is a Mediterranean medicinal plant traditionally cultivated for its ethnopharmacological benefits, such as antidiarrheal, antidiabetic, enhance acetylcholine, antioxidant, antiatherosclerotic, and for its possible anti-neurodegenerative potential. The aim of the present study was to evaluate the chemical composition, as well as the cognitive-enhancing, anxiolytic, and antioxidant activities of the aqueous extract from C. siliqua (CsAE) leaves against 6-hydroxydopamine (6-OHDA) zebrafish Parkinson’s disease (PD) model. CsAE (0.1, 0.3, and 1 mg/L) was administered by immersion to zebrafish (Danio rerio) for eight consecutive days and one hour before each behavioral test of each day, while 6-OHDA (250 µM) treatment was supplied one day before the novel tank diving test (NTT). Qualitative and quantitative analyses were performed by the ultra-high-performance liquid chromatography (UHPLC) analysis. The memory performance was evaluated through the NTT and Y-maze tests. Additionally, the in vitro and in vivo antioxidant status and acetylcholinesterase (AChE) activity was also assessed. Our finds demonstrated that CsAE presented positive antioxidant and anti-AChE activities, which contributed to the improvement of cognitive function in the 6-OHDA zebrafish PD model.

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