scholarly journals Profiling the circulating miRNAs in mice exposed to gram-positive and gram-negative bacteria by Illumina small RNA deep sequencing

2015 ◽  
Vol 22 (1) ◽  
pp. 1 ◽  
Author(s):  
Cheng-Shyuan Rau ◽  
Shao-Chun Wu ◽  
Johnson Yang ◽  
Tsu-Hsiang Lu ◽  
Yi-Chan Wu ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Gram Negative Bacteria ◽  
Circulating Mirnas ◽  
Gram Positive ◽  
Gram Negative ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

scholarly journals Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Shao-Chun Wu ◽  
Cheng-Shyuan Rau ◽  
Johnson Chia-Shen Yang ◽  
Tsu-Hsiang Lu ◽  
Yi-Chan Wu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Deep Sequencing ◽  
Small Rna ◽  
Small Rnas ◽  
Biological Function ◽  
Circulating Mirnas ◽  
Serum Samples ◽  
Circulating Micrornas ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

Background. This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation in the presence and absence of FK506 immunosuppression.Methods. A 1 cm BALB/c donor sciatic nerve graft was transplanted into the sciatic nerve gaps created in recipient C57BL/6 mice with or without daily FK506 immunosuppression [1 mg/(kg·d)]. At 3, 7, and 14 d after nerve allotransplantation, serum samples were collected for miRNA expression analysis by Illumina small RNA deep sequencing.Results. Sequence analysis showed that the dominant size of circulating small RNAs after nerve allotransplantation was 22 nucleotides, followed by 23-nucleotide sequences. Nine upregulated circulating miRNAs (let-7e-5p, miR-101a-3p, miR-151-5p, miR-181a-5p, miR-204-5p, miR-340-5p, miR-381-3p, miR-411-5p, miR-9-5p, and miR-219-2-3p) were identified at 3 d, but none was identified at 7 or 14 d. Among them, miR-9-5p had the highest fold-change of >50-fold, followed by miR-340-5p with 38.8-fold. The presence of these nine miRNAs was not significant at 7 and 14 d after nerve allotransplantation with or without immunosuppression, showing that these miRNAs are not ideal biomarkers for monitoring rejection of deep-buried nerve allografts, a response usually observed later.Conclusions. We identified nine upregulated circulating miRNAs, which may have a biological function, particularly during the early stages after nerve allotransplantation under FK506 immunosuppression.

Identification of viruses infecting sweet potato in southern China by small RNA deep sequencing and PCR detection

2018 ◽  
Vol 85 (2) ◽  
pp. 122-127 ◽  
Author(s):  
Siqi Ma ◽  
Qiufeng Zheng ◽  
Jiajie Ye ◽  
Wendi Feng ◽  
Guohui Zhou ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Deep Sequencing ◽  
Small Rna ◽  
Southern China ◽  
Pcr Detection ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

Small RNA Deep Sequencing and the Effects of microRNA408 on Root Gravitropic Bending in Arabidopsis

2015 ◽  
Vol 27 (6) ◽  
pp. 495-503 ◽  
Author(s):  
Huasheng Li ◽  
Jinying Lu ◽  
Qiao Sun ◽  
Yu Chen ◽  
Dacheng He ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

scholarly journals Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing

BioTechniques ◽  
2010 ◽  
Vol 49 (4) ◽  
pp. 751-755 ◽  
Author(s):  
Mitsuoki Kawano ◽  
Chika Kawazu ◽  
Marina Lizio ◽  
Hideya Kawaji ◽  
Piero Carninci ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing
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