scholarly journals Coiled-coil inspired functional inclusion bodies

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Marcos Gil-Garcia ◽  
Susanna Navarro ◽  
Salvador Ventura
Keyword(s):  
Inclusion Bodies ◽  
Coiled Coil ◽  
Functional Inclusion

scholarly journals Positive in vitro wound healing effects of functional inclusion bodies of a lipoxygenase from the Mexican axolotl

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Anne Stamm ◽  
Sarah Strauß ◽  
Peter Vogt ◽  
Thomas Scheper ◽  
Iliyana Pepelanova
Keyword(s):  
Wound Healing ◽  
Inclusion Bodies ◽  
Mexican Axolotl ◽  
Functional Inclusion

Fusion of a Coiled-Coil Domain Facilitates the High-Level Production of Catalytically Active Enzyme Inclusion Bodies

ChemCatChem ◽  
2015 ◽  
Vol 8 (1) ◽  
pp. 142-152 ◽  
Author(s):  
Martin Diener ◽  
Benita Kopka ◽  
Martina Pohl ◽  
Karl-Erich Jaeger ◽  
Ulrich Krauss
Keyword(s):  
Inclusion Bodies ◽  
Coiled Coil ◽  
Active Enzyme ◽  
Coiled Coil Domain ◽  
Catalytically Active ◽  
High Level ◽  
Level Production

Nanopills: Functional Inclusion Bodies Produced in Bacteria as Naturally Occurring Nanopills for Advanced Cell Therapies (Adv. Mater. 13/2012)

2012 ◽  
Vol 24 (13) ◽  
pp. 1741-1741
Author(s):  
Esther Vázquez ◽  
José L. Corchero ◽  
Joan F. Burgueño ◽  
Joaquin Seras-Franzoso ◽  
Ana Kosoy ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
Cell Therapies ◽  
Naturally Occurring ◽  
Functional Inclusion

scholarly journals Production of functional inclusion bodies in endotoxin-free Escherichia coli

2014 ◽  
Vol 98 (22) ◽  
pp. 9229-9238 ◽  
Author(s):  
Fabián Rueda ◽  
Olivia Cano-Garrido ◽  
Uwe Mamat ◽  
Kathleen Wilke ◽  
Joaquin Seras-Franzoso ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Functional Inclusion

scholarly journals Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

2019 ◽  
Vol 6 (18) ◽  
pp. 1900849 ◽  
Author(s):  
Mireia Pesarrodona ◽  
Toni Jauset ◽  
Zamira V. Díaz‐Riascos ◽  
Alejandro Sánchez‐Chardi ◽  
Marie‐Eve Beaulieu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Inclusion Bodies ◽  
Local Administration ◽  
Functional Inclusion

Enzyme assembly guided by SPFH‐induced functional inclusion bodies for enhanced cascade biocatalysis

2020 ◽  
Vol 117 (5) ◽  
pp. 1446-1457 ◽  
Author(s):  
Xueqin Lv ◽  
Ke Jin ◽  
Yaokang Wu ◽  
Cheng Zhang ◽  
Shixiu Cui ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
Cascade Biocatalysis ◽  
Functional Inclusion

scholarly journals In Vivo Enzyme Immobilization by Inclusion Body Display

2010 ◽  
Vol 76 (16) ◽  
pp. 5563-5569 ◽  
Author(s):  
Björn Steinmann ◽  
Andreas Christmann ◽  
Tim Heiseler ◽  
Janine Fritz ◽  
Harald Kolmar
Keyword(s):  
Inclusion Body ◽  
Inclusion Bodies ◽  
Active Form ◽  
Coiled Coil ◽  
Cost Effective ◽  
Galactose Oxidase ◽  
Novel Strategy ◽  
High Level ◽  
Carboxy Terminal

ABSTRACT A novel strategy for in vivo immobilization of enzymes on the surfaces of inclusion bodies has been established. It relies on expression in Escherichia coli of the polyhydroxybutyrate synthase PhaC from Cupriavidus necator, which carries at its amino terminus an engineered negatively charged α-helical coil (Ecoil) and forms inclusion bodies upon high-level expression. Coexpression in the same cell of galactose oxidase (GOase) from Fusarium spp. carrying a carboxy-terminal positively charged coil (lysine-rich coil [Kcoil]) sequence results in heterodimeric coiled-coil formation in vivo and in the capture of the enzyme in active form on the surface of the inclusion body particle. These round-shaped enzyme-decorated microparticles, with sizes of approximately 0.7 μm, can be isolated from lysed cells simply by centrifugation. The cost-effective one-step generation and isolation of enzymes immobilized on inclusion body particles may become useful for various applications in bioprocessing and biotransformation.

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