scholarly journals Francisella philomiragia bacteremia in an immunocompromised patient: a rare case report

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Hui Shan Chua ◽  
Yih Harng Soh ◽  
Shih Keng Loong ◽  
Sazaly AbuBakar
Keyword(s):  
Mass Spectrometry ◽  
16S Rdna ◽  
Acute Pulmonary Oedema ◽  
Molecular Technique ◽  
Type I ◽  
16S Rdna Sequencing ◽  
Maldi Tof Mass Spectrometry ◽  
Prompt Treatment ◽  
Maldi Tof ◽  
Rdna Sequencing

Abstract Background Francisella philomiragia is a very rare opportunistic pathogen of humans which causes protean diseases such as pneumonia and other systemic infections. Subsequent failure of prompt treatment may result in poor prognosis with mortality among infected patients. Case presentation The present report describes a case of F. philomiragia bacteraemia first reported in Malaysia and Asian in a 60-year-old patient with underlying end-stage renal disease (ESRF) and diabetes mellitus. He presented with Acute Pulmonary Oedema with Non-ST-Elevation Myocardial Infarction (NSTEMI) in our hospital. He was intubated in view of persistent type I respiratory failure and persistent desaturation despite post haemodialysis. Blood investigation indicated the presence of ongoing infection and inflammation. The aerobic blood culture growth of F. philomiragia was identified using the matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (Score value: 2.16) and confirmed by 16S Ribosomal DNA (16S rDNA) sequencing. He was discharged well on day 26 of admission, after completing one week of piperacillin/tazobactam and two weeks of doxycycline. Conclusion Clinical suspicion should be raised if patients with known risk factors are presenting with pneumonia or pulmonary nodules especially as these are the most common manifestations of F. philomiragia infection. Early diagnosis via accurate laboratory identification of the organism through MALDI-TOF mass spectrometry and molecular technique such as 16S rDNA sequencing are vital for prompt treatment that results in better outcomes for the afflicted patients.

scholarly journals MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples

2021 ◽  
Vol 8 ◽  
Author(s):  
Thomas Brauge ◽  
Sylvain Trigueros ◽  
Arnaud Briet ◽  
Sabine Debuiche ◽  
Guylaine Leleu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
16S Rdna ◽  
Sea Water ◽  
Species Level ◽  
16S Rdna Sequencing ◽  
Genus Level ◽  
Maldi Tof ◽  
Rdna Sequencing ◽  
Seafood Products ◽  
Tof Ms

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) associated with the Bruker BioTyperTM V7.0.0 database for the identification of 713 bacterial strains isolated from seafood products and sea water samples (ANSES B3PA collection) under culture conditions that may have been significantly different from those used to create the reference spectrum vs. the 16S rDNA sequencing. We identified 78.8% of seafood isolates with 46.7% at the species level (Bruker score above 2) and 21.2% (Bruker score between 1.7 and 2) at the genus level by the two identification methods, except for 3.8% of isolates with a difference of identification between the two methods (Bruker score between 1.7 and 2). There were 41.9% isolates (Bruker score below 1.7) with the identification at the genus level. We identified 94.4% of seafood isolates with 16S rDNA sequencing. The MALDI-TOF allowed a better strain identification to the species level contrary to the 16s rDNA sequencing, which allowed an identification mainly to the genus level. MALDI-TOF MS in association with the Bruker database and 16S rDNA sequencing are powerful tools to identify a wide variety of bacteria from seafood but require further identification by biochemical, molecular technique or other conventional tests.

Rapid Identification of Microorganisms Isolated from Pickled Vegetables by MALDI-TOF Mass Spectrometry

2013 ◽  
Vol 712-715 ◽  
pp. 494-497
Author(s):  
Zhi Lei Tan ◽  
Yu Qiao Wei ◽  
Shuang Liang ◽  
Ran Zhang ◽  
Miao Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
16S Rdna ◽  
Leuconostoc Mesenteroides ◽  
16S Rdna Sequencing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rdna Sequencing ◽  
Pickled Vegetables ◽  
Food Bacteria ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time-off light mass spectrometry (MALDI-TOF MS) is increasingly used as a microbial diagnostic method for species identification of pathogens. However, MALDI-TOF MS identification of food bacteria was seldom reported. Ten strains isolated from different pickled vegetables were rapid identified by MALDI-TOF MS. The results of MALDI-TOF MS were confirmed by 16S rDNA sequencing method. Different score values in MALDI-TOF MS revealed nineLeuconostoc mesenteroides and oneStaphylococcus.Identification success at the species and genus levels was 90% (9/10) and 100% (10/10), respectively.16S rDNA sequencing results showed that nine stains were identified asLeuconostoc mesenteroides and one wasStaphylococcus saprophyticus.Results obtained demonstrate that MALDI-TOFMS is a promising method for discriminating and identifying food bacteria.

scholarly journals Comparative analysis of Campylobacter isolates from wild birds and chickens using MALDI-TOF MS, biochemical testing, and DNA sequencing

2018 ◽  
Vol 30 (3) ◽  
pp. 354-361 ◽  
Author(s):  
Samantha J. Lawton ◽  
Allison M. Weis ◽  
Barbara A. Byrne ◽  
Heather Fritz ◽  
Conor C. Taff ◽  
...  
Keyword(s):  
16S Rdna ◽  
Phylogenetic Relationships ◽  
Wild Birds ◽  
16S Rdna Sequencing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Gene Testing ◽  
Rdna Sequencing ◽  
Biochemical Testing ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1). The test results for all 27 isolates were in 100% agreement between MALDI-TOF MS, the combined results of 16S rDNA sequencing, and the hippurate hydrolysis gene PCR ( p = 0.0027, kappa = 1). Likewise, the identifications derived from WGS from a subset of 14 isolates were in 100% agreement with the MALDI-TOF MS identification. In contrast, biochemical testing misclassified 5 isolates of C. jejuni as C. coli, and 16S rDNA sequencing alone was not able to differentiate between C. coli and C. jejuni for 11 sequences ( p = 0.1573, kappa = 0.0857) when compared to MALDI-TOF MS and WGS. No agreement was observed between MALDI-TOF MS dendrograms and the phylogenetic relationships revealed by rDNA sequencing or WGS. Our results confirm that MALDI-TOF MS is a fast and reliable method for identifying Campylobacter isolates to the species level from wild birds and chickens, but not for elucidating phylogenetic relationships among Campylobacter isolates.

scholarly journals Contraception: Influence on Vaginal Microbiota and Identification of Vaginal Lactobacilli Using MALDI-TOF MS and 16S rDNA Sequencing

2018 ◽  
Vol 12 (1) ◽  
pp. 218-229 ◽  
Author(s):  
Sonia E. Fosch ◽  
Cecilia A. Ficoseco ◽  
Antonella Marchesi ◽  
Silvina Cocucci ◽  
Maria E. F. Nader-Macias ◽  
...  
Keyword(s):  
16S Rdna ◽  
Oral Contraceptive Pill ◽  
Vaginal Microbiota ◽  
16S Rdna Sequencing ◽  
Contraceptive Methods ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Vaginal Lactobacilli ◽  
Rdna Sequencing ◽  
Tof Ms

Background:The vaginal microbiome is influenced by a wide variety of factors, including contraceptive methods.Aim:To evaluate the effect of contraceptive methods on vaginal microbiota and to compare MALDI-TOF MS and 16S rDNA sequencing for lactobacilli identification.Patients and Methods:One hundred and one (101) women consulting for birth control were included in a prospective study. Their vaginal content was sampled and analyzed once before they started using the contraceptive method of their choice, and twice after the initiation of contraception, at three months (94/101 women attended) and at six months (89/101 women attended). The relative frequencies of yeasts and trichomonas were analyzed. MALDI-TOF MS and 16S rDNA sequence analysis were applied for the identification of lactobacilli in their vaginal microbiota. The following contraceptive methods were assessed: Combined Oral Contraceptive Pill (COCP), Condom (CON) and The Rhythm Method (RHYT). McNemar’s statistical test was applied.Results:A statistically significant association between COCP and normal microbiota was observed after three months (p< 0.01) and after six months (p< 0.0001), when the vaginal microbiota was modified. At six months, inflammatory reaction was detected in 3/7 women in the CON group, while 6/7 patients using RHYT showed the same state. Yeast colonization increased with the COCP. Identification of lactobacilli by MALDI-TOF MS analysis compared to 16S rDNA sequencing yielded 92.9% concordant results.Lactobacillus gasseriandL. crispatuswere the predominant species.Conclusion:The pattern of vaginal states was significantly modified. Hormone administration apparently corrected the alterations and retained a normal vaginal state. MALDI-TOF MS has the potential of being an accurate tool for the identification of vaginal lactobacilli speciesL. murinuswas for the first time isolated from the vagina.

Comparison of MALDI-TOF MS Biotyper and 16S rDNA sequencing for the identification of Pseudomonas species isolated from fish

2019 ◽  
Vol 132 ◽  
pp. 313-318 ◽  
Author(s):  
Miroslava Kačániová ◽  
Alīna Klūga ◽  
Attila Kántor ◽  
Juraj Medo ◽  
Jana Žiarovská ◽  
...  
Keyword(s):  
16S Rdna ◽  
16S Rdna Sequencing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rdna Sequencing ◽  
Pseudomonas Species ◽  
Tof Ms

scholarly journals Rapid species-level identification of vaginal and oral lactobacilli using MALDI-TOF MS analysis and 16S rDNA sequencing

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Annette Carola Anderson ◽  
Mohamed Sanunu ◽  
Christian Schneider ◽  
Andreas Clad ◽  
Lamprini Karygianni ◽  
...  
Keyword(s):  
16S Rdna ◽  
Species Level ◽  
16S Rdna Sequencing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Rdna Sequencing ◽  
Level Identification ◽  
Tof Ms

scholarly journals Identification of airborne bacteria by 16S rDNA sequencing, MALDI-TOF MS and the MIDI microbial identification system

Aerobiologia ◽  
2015 ◽  
Vol 31 (3) ◽  
pp. 271-281 ◽  
Author(s):  
Else Marie Fykse ◽  
Torbjörn Tjärnhage ◽  
Tarmo Humppi ◽  
Vilde Sørvik Eggen ◽  
Andre Ingebretsen ◽  
...  
Keyword(s):  
16S Rdna ◽  
Identification System ◽  
Airborne Bacteria ◽  
16S Rdna Sequencing ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Rdna Sequencing ◽  
Microbial Identification System ◽  
Tof Ms

Identification of indigenous Lactobacillus isolated from artisanal Algerian dairy product by 16S rRNA Gene Sequencing and MALDI-TOF Mass Spectrometry

2020 ◽  
Vol 9 (5) ◽  
pp. 193-206
Author(s):  
Ait Abdeslam Arezki ◽  
Desmasures Nathalie ◽  
Bensoltane Ahmed
Keyword(s):  
Mass Spectrometry ◽  
16S Rdna ◽  
Dairy Products ◽  
Dairy Product ◽  
Universal Primers ◽  
Phenotypic Characterization ◽  
Lactobacillus Species ◽  
Rrna Gene ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

El-klila, Jben, Rayeb and Lben are artisanal dairy products typical of the North African region. These products are considered a good source of nutrients and a vector par excellence of indigenous lactic acid bacteria with significant technological and therapeutic potential. In this study, fifty-two Lactobacillus isolates from traditional dairy products (El-Klila, Jben and Rayeb) from the Ain Sefra region (southwestern of Algeria) were identified by phenotypic, genotypic and MALDI-TOF mass spectrometry methods. Phenotypic characterization allowed dividing the isolates into two groups: 7.69% of isolates mesophilic and strict heterofermentative and 92.31% isolates mesophilic and optional heterofermentative. The isolates identification by sequencing 16S rDNA was performed using the universal primers w02 and w18. Comparing the sequences of the isolates with the GenBank database (NCBI) revealed a high percentage with five species of Lactobacillus. Of the 52 isolates, thirteen were identified as Lactobacillus paracasei, four isolates were L. brevis, five isolates were L. rhamnosus and ten isolates were L. plantarum. The lasting twenty isolates were related, at the same time, to the plantarum species and the pentosus species. The use of MALDI-TOF mass spectrometry confirmed the identity of 36 strains of Lactobacillus. Of the 20 isolates related to the plantarum / pentosus group by 16S rDNA, 50% were identified as L. plantarum. Finally, the results of the two combined methods allowed us to correctly identify 80.76% of the Lactobacillus isolates at the genus and species level. The analysis by MALDI-TOF SM has proved to be a very reliable, fast (less than an hour) and inexpensive tool for identifying native Lactobacillus species from artisanal dairy products. This method can constitute a major tool for the study of the microbial biodiversity of artisanal food products.

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