scholarly journals Human papillomavirus genotype distribution in Ethiopia: an updated systematic review

2022 ◽  
Vol 19 (1) ◽  
Author(s):  
Awoke Derbie ◽  
Daniel Mekonnen ◽  
Endalkachew Nibret ◽  
Melanie Maier ◽  
Yimtubezinash Woldeamanuel ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papillomaviruses ◽  
Low Risk ◽  
Scientific Quality ◽  
Genotype Distribution ◽  
Hpv 16 ◽  
Hpv Genotypes ◽  
Hpv Types ◽  
Hpv 18

Abstract Background Cervical cancer is caused by infection with high-risk human papillomaviruses (HR-HPVs). It is one of the leading causes of cancer-related deaths in Ethiopia and globally. To develop efficient vaccination and HPV-based cervical cancer screening approaches, data on genotype distribution of HPVs is crucial. Hence, the study was aimed to review HPV genotype distribution in Ethiopia. Methods Research articles were systematically searched using comprehensive search strings from PubMed/Medline and SCOPUS. Besides, Google Scholar was searched manually for grey literature. The last search was conducted on 18 August 2021. The first two authors independently appraised the studies for scientific quality and extracted the data using Excel sheet. The pooled HPV genotype distribution was presented with descriptive statistics. Results We have included ten studies that were reported from different parts of the country during 2005 and 2019. These studies included 3633 women presented with different kinds of cervical abnormalities, from whom 29 different HPV genotypes with a sum of 1926 sequences were reported. The proportion of high-risk, possible/probable high-risk and low-risk HPVs were at 1493 (77.5%), 182 (9.4%) and 195 (10.1%), respectively. Of the reported genotypes, the top five were HPV 16 (37.3%; 95% CI 35.2.1–39.5%), HPV 52 (6.8%; 95% CI 5.8–8.0%), HPV 35 (4.8%; 95% CI 3.9–5.8%), HPV 18 (4.4%; 95% CI 3.5–5.3%) and HPV 56 (3.9%: 95% CI 3.1–4.9%). Some of other HR-HPV groups include HPV 31 (3.8%), HPV 45 (3.5%), HPV 58 (3.1%), HPV 59(2.3%), and HPV 68 (2.3%). Among the high-risk types, the combined prevalence of HPV 16/18 was at 53.7% (95% CI 51.2–56.3%). HPV 11 (2.7%: 95% CI 2.1–3.5%), HPV 42 (2.1%: 95% CI 1.5–2.8%) and HPV 6 (2.1%: 95% CI 1.4–2.7%) were the most common low-risk HPV types. Conclusions We noted that the proportion of HR-HPV types was higher and HPV 16 in particular, but also HPV 52, HPV 35 and HPV 18, warrant special attention in Ethiopian’s vaccination and HPV based cervical screening program. Additional data from other parts of the country where there is no previous HPV genotype report are needed to better map the national HPV genotypes distribution of Ethiopia.

scholarly journals Expression of the high-risk human papillomavirus type 18 and 45 E7 oncoproteins in cervical carcinoma biopsies

2005 ◽  
Vol 86 (12) ◽  
pp. 3235-3241 ◽  
Author(s):  
Marc Fiedler ◽  
Sigrun Ressler ◽  
Beatriz Campo-Fernández ◽  
Andreas Laich ◽  
Lars Jansen ◽  
...  
Keyword(s):  
High Risk ◽  
Cervical Carcinoma ◽  
Human Papillomaviruses ◽  
Hpv 16 ◽  
Cervical Cancers ◽  
Hpv Types ◽  
High Level ◽  
E7 Proteins ◽  
High Risk Hpv ◽  
Hpv 18

E7 proteins are major oncoproteins of high-risk human papillomaviruses (HPVs), which play a key role in cervical carcinogenesis. These proteins have been shown to immortalize primary human cells. Due to the absence of antibodies with suitable sensitivity and specificity, little is known about expression of the E7 oncoproteins in naturally infected tissues. Recently, high-level expression of the E7 protein of HPV-16, the most prevalent oncogenic HPV type, was demonstrated in cervical carcinomas by immunohistochemistry; however, approximately 15 additional high-risk HPV types are known to be associated with cervical carcinoma. It is unknown whether the E7 oncoproteins of HPV-18 and -45, the second and third most prevalent HPV types, are expressed in cervical cancers. Using antibodies against HPV-18 and -45 E7 proteins, it is shown here for the first time that the HPV-18 and -45 E7 proteins can be detected in cervical carcinoma biopsies. Together with anti-HPV-16 E7 antibodies, this could create the possibility of detecting E7 oncoproteins in approximately 80 % of all cervical cancers.

scholarly journals Phylogeny of HPV-16 and HPV-18 Multiple Infection of a Patient with Cervical Cancer from Dr. Hasan Sadikin General Hospital, Bandung: A Case Report

2018 ◽  
Vol 10 (3) ◽  
pp. 284-9
Author(s):  
Vera Amalia Lestari ◽  
Ika Agus Rini ◽  
Gita Widya Pradini ◽  
Edhyana Sahiratmadja ◽  
Herman Susanto
Keyword(s):  
Cervical Cancer ◽  
General Hospital ◽  
Human Papillomaviruses ◽  
Cervical Cancer Patient ◽  
Multiple Infection ◽  
Single Infection ◽  
Cervical Tissue ◽  
Hpv 16 ◽  
Hpv Genotypes ◽  
Hpv 18

BACKGROUND: From all of human papillomaviruses (HPV) genotypes capable of causing cervical cancer, it is estimated that 70 percent are HPV-16 and HPV-18. HPV-16 can infect the tissues in single infection or together with other high-risk types of HPV, and the most common is with HPV-18. The origin of HPV can be identified by its phylogenetic tree. The aim of this study was to determine the phylogeny of HPV-16 and HPV-18 multiple infection in cervical cancer, whether both HPVs were from the same origin.METHODS: Cervical tissue biopsies (n=33) were obtained from Hasan Sadikin Hospital in the period of September to November 2016. HPV genotyping test was performed to confirm the HPV-16 and HPV-18 multiple infection. L1 gene of both HPV-16 and HPV-18 were sequenced for phylogenetic analysis.RESULTS: Phylogenetic analysis of L1 HPV-16 and HPV-18 showed the closest relationship with sequence from China and Thailand, respectively.CONCLUSION: HPV-16 and HPV-18 multiple infection of a cervical cancer patient from Dr. Hasan Sadikin General Hospital Bandung showed a very close L1 phylogeny relationship with isolate from Asian region.KEYWORDS: HPV-16, HPV-18, multiple infection, cervical cancer, Bandung

scholarly journals Human Papillomavirus (HPV) Type 18 Induces Extended Growth in Primary Human Cervical, Tonsillar, or Foreskin Keratinocytes More Effectively than Other High-Risk Mucosal HPVs

2009 ◽  
Vol 83 (22) ◽  
pp. 11784-11794 ◽  
Author(s):  
Michael J. Lace ◽  
James R. Anson ◽  
Aloysius J. Klingelhutz ◽  
John H. Lee ◽  
Aaron D. Bossler ◽  
...  
Keyword(s):  
High Risk ◽  
Human Keratinocytes ◽  
Human Papillomaviruses ◽  
Population Doubling ◽  
Primary Human Keratinocytes ◽  
Hpv 16 ◽  
Epithelial Origin ◽  
Hpv Types ◽  
Population Doublings ◽  
Hpv 18

ABSTRACT Mucosal high-risk (HR) human papillomaviruses (HPVs) that cause cervical and other anogenital cancers also are found in ∼25% of head and neck carcinomas (HNCs), especially those arising in the oropharynx and the tonsils. While many HR HPV types are common in anogenital cancer, over 90% of HPV-positive HNCs harbor HPV type 16 (HPV-16). Using a quantitative colony-forming assay, we compared the ability of full-length mucosal HPV genomes, i.e., the low-risk HPV-11 and HR HPV-16, -18, and -31, to persist in and alter the growth of primary human keratinocytes from the foreskin, cervix, and tonsils. The HR HPV types led to the formation of growing keratinocyte colonies in culture independent of the site of epithelial origin. However, HPV-18 induced colony growth in all keratinocytes >4-fold more effectively than HPV-16 or HPV-31 and >20-fold more efficiently than HPV-11 or controls. HPV-11-transfected or control colonies failed to expand beyond 32 to 36 population doublings postexplantation. In contrast, individual HR HPV-transfected clones exhibited no apparent slowdown of growth or “crisis,” and many maintained HPV plasmid persistence beyond 60 population doublings. Keratinocyte clones harboring extrachromosomal HR HPV genomes had shorter population doubling times and formed dysplastic stratified epithelia in organotypic raft cultures, mirroring the pathological features of higher-grade intraepithelial lesions, yet did not exhibit chromosomal instability. We conclude that, in culture, the HR HPV type, rather than the site of epithelial origin of the cells, determines the efficacy of inducing continued growth of individual keratinocytes, with HPV-18 being the most aggressive mucosal HR HPV type tested.

scholarly journals The Distribution and Prevalence of High-Risk HPV Genotypes Other than HPV-16 and HPV-18 among Women Attending Gynecologists’ Offices in Kazakhstan

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 794
Author(s):  
Gulzhanat Aimagambetova ◽  
Aisha Babi ◽  
Alpamys Issanov ◽  
Sholpan Akhanova ◽  
Natalya Udalova ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Cervical Cancer Prevention ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Hpv 18 ◽  
Great Burden ◽  
Epidemiological Situation

Cervical cancer represents a great burden to public health of women. This study aimed to obtain a nationwide genotyping survey and analysis of high risk-HPV including those that are caused by HPV types other than HPV-16 and HPV-18, among women in Kazakhstan. This study was conducted based on the collection of survey and cervical swabs of 1645 women across the country. The samples were genotyped for high-risk HPV types based on real-time PCR methods. Collected data was analyzed with the focus on high-risk HPV types other than HPV-16 and -18. Infection was present in 22% of women who participated in the study. The most prevalent types were HPV-31 among single infections and HPV-68 among multiple infections. Conclusively, despite the lack of attention high-risk HPV types beyond HPV-16 and -18 get in attempts of cervical cancer prevention in Kazakhstan, their prevalence is high and plays a large role in cervical cancer epidemiological situation.

High Risk Human Papilloma Virus Genotype Distribution in Cervical Intraepithelial and Invasive Carcinoma

2020 ◽  
Author(s):  
Nazish Jaffar
Keyword(s):  
High Risk ◽  
Hybridization Technique ◽  
Low Grade ◽  
Genotype Distribution ◽  
High Grade ◽  
Virus Genotype ◽  
Hpv 16 ◽  
Detection Techniques ◽  
Hpv Genotypes ◽  
Hpv 18

Background: High-risk (HR) Human Papillomavirus (HPV) is an established cause of cervical cancer. HPV genotype detection is significant in preventing cervical cancers through targeted vaccination our study aimed to identify HRHPV16/18 and non 16/18 in cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC). Methods: A retrospective study was performed at Pathology Department, BMSI, JPMC, Karachi. About 96 cases of CIN and SCC were included. Analysis of HPV genotypes was performed by DNA extraction, PCR amplification and flow-through hybridization technique. The probes used had a cluster of 13 HRHPV into a group of 3 as HPV HR 1, 2 and 3. Chi square/ Fischer Exact test were applied to observe the association of morphological types of the lesion and expression of HPV genotypes. Results: HPV DNA positivity was 44% in our series. HPV HR 1 was observed in majority of cases (61.9%), followed by HPV 16 in 23.8%, HPV HR 3 in 9.5%, and HPV HR 2 in 4.7% cases respectively. The unique finding was absence of HPV 18 in the series. High grade lesions and invasive cancers showed positivity for HPV HR 1 and HPV 16, while low grade lesions were positive for HPV HR 1, 2 and 3 respectively. Conclusion: HPV HR1 are major causative agents for low and high grade intraepithelial and invasive SCC, followed by HPV 16. Absence of HPV 18 was the novel finding. Our results differ from studies within and outside the region, suggestive of diversified genetic makeup and impact of detection techniques on results.

scholarly journals HPV genotypes and epidemiology in women cervical cancer in Senegal.  

2021 ◽  
Author(s):  
Khadidiatou Niane ◽  
Cheikh Tidiane Diagne ◽  
Gora Diop ◽  
Ndongo Dia ◽  
Cheikh Talla ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Causes Of Death ◽  
Sub Saharan Africa ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Hpv Genotypes ◽  
Hpv Types ◽  
Sub Saharan ◽  
High Risk Hpv

Abstract Background Cases of cervical cancer are increasing steadily in sub-Saharan Africa, with over 75,000 new cases and nearly 50,000 deaths a year (Mboumba et al., 2017). In Senegal, pathologies such as cervical cancer are one of the top causes of death and the Human papillomavirus (HPV) is its aetiological agent (Steenbergen et al., 2005). Methods The aim of the study is to analyse the distribution of HPV among Senegalese women with cervical cancer. The main objective of this study is to identify the HPV types associated or “co-associated” with cervical oncogenesis in Senegal. The association with the risk factors of cervin carcinogenesis were analysed as well. Cervical biopsies were performed on the women admitted to Aristide Hospital Le Dantec-Julio Curie Institute. Three methods were used to detect HPV genotypes: SANGER sequencing genotyping (Applied BioSystems), PCR real-time approach technique (HPV 16 & 18 RealTime PCR kit) (www.bioneer.co.kr) and the genotyping approach from Chippron (HPV kit 3.5 LCDArray) ([email protected]).Results In this study, 24.16% of monoinfections and 75.83% of multiple infections (co-infections) were noted and the majority were at high risk (HR-HPV types). It appears that the HPV genotypes 16, 18 and 45 are the most found in tumors. The most common types of HPV in our study were HPV 16 (100%), 18 (83%), 45 (33%), 33 (31%), 59 (28%), 35 (12%), 31 (11%), 58 (8%), 39 and 73 (4%), 44, 54 and 68 (3%). In the Dakar region, which had the highest number of cases, a prevalence of 17.89% of HR-HPV co-infections was found and the majority of our patients were on a polygamous diet. Polygamy could therefore be a cofactor in the occurrence of cervical cancer in Senegalese women.Conclusion Polygamy could represent a cofactor in the occurrence of cervical cancer in Senegalese women. No association was found between high-risk HPV co-infections and cancer stages. However, an increase of our cohort would be necessary to affirm these hypotheses.

scholarly journals Genotype Distribution of Human Papillomavirus among Women with Cervical Cytological Abnormalities or Invasive Squamous Cell Carcinoma in a High-Incidence Area of Esophageal Carcinoma in China

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Yuanyuan Wang ◽  
Shaohong Wang ◽  
Jinhui Shen ◽  
Yanyan Peng ◽  
Lechuan Chen ◽  
...  
Keyword(s):  
High Risk ◽  
Cervical Cytology ◽  
Invasive Squamous Cell Carcinoma ◽  
Genotype Distribution ◽  
Normal Cytology ◽  
Hpv 16 ◽  
High Incidence ◽  
Hpv Types ◽  
High Risk Hpv ◽  
High Incidence Area

Data of HPV genotype including 16 high-risk HPV (HR-HPV) and 4 low-risk HPV from 38,397 women with normal cytology, 1341 women with cervical cytology abnormalities, and 223 women with ISCC were retrospectively evaluated by a hospital-based study. The prevalence of high-risk HPV (HR-HPV) was 6.51%, 41.83%, and 96.86% in women with normal cytology, cervical cytology abnormalities, and ISCC, respectively. The three most common HPV types were HPV-52 (1.76%), HPV-16 (1.28%), and HPV-58 (0.97%) in women with normal cytology, whereas the most prevalent HPV type was HPV-16 (16.85%), followed by HPV-52 (9.55%) and HPV-58 (7.83%) in women with cervical cytology abnormalities. Specifically, HPV-16 had the highest frequency in ASC-H (24.16%, 36/149) and HSIL (35.71%, 110/308), while HPV-52 was the most common type in ASC-US (8.28%, 53/640) and LSIL (16.80%, 41/244). HPV-16 (75.78%), HPV18 (10.31%), and HPV58 (9.87%) were the most common types in women with ISCC. These data might contribute to increasing the knowledge of HPV epidemiology and providing the guide for vaccine selection for women in Shantou.

scholarly journals Prevalence of precancerous cervical lesions and high-risk human papillomavirus types in Yaounde, Cameroon

2021 ◽  
Vol 15 (09) ◽  
pp. 1339-1345
Author(s):  
Richard Tagne Simo ◽  
Arsène G Djoko Nono ◽  
Hervet Paulin Fogang Dongmo ◽  
Paul F Seke Etet ◽  
Bertrand Kiafon Fonyuy ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Precancerous Lesions ◽  
Health Centre ◽  
Oral Contraceptive Pill ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Hpv Types ◽  
Cytologic Abnormalities ◽  
Hpv 18

Introduction: Various Human papillomavirus (HPV) types cause cervical cancer, and represent the primary cause of cancer death in Africa and the second cause of most common cancers in Cameroon. Herein, we determined the prevalence of high-risk HPV types in women and associated cervical cytologic abnormalities in Yaounde, Cameroon. Methodology: A cross-sectional study targeting HPV-positive women aged 20 and over was conducted between March and June 2020 at the Saint Martin de Porres’ Health Centre in Yaounde. HPV tests were performed by PCR for detection of HPVs 16, 18, 33, and 45. The test was performed on 616 women using exfoliated cell specimens; then, we processed on cytological diagnosis with Pap smears on HPV positive specimens. Results: The HPV types tested were detected in 137 participants, of which 38.7% with multiple HPV infections, and the remaining part with single HPV infections of type HPV 16 (28.5%), HPV 18 (17.5%), HPV 33 (10.2%), and HPV 45 (5.1%). Cervical cytologic abnormalities were found in 69.34% of participants including: LSIL (49.63%), HSIL (15.32%), ASC-US (3.66%) and AGC (0.73%). Co-infections with HPV 16 and HPV 18 were significantly associated with HSIL (p = 0.001) lesions, while HPV 45 was more common in participants with normal cytology (p = 0.001). Cervical lesion occurrence was significantly associated with the number of sexual partners (p = 0.02) and history of oral contraceptive pill use (p = 0.001). Conclusions: Our results suggest that HPV 16 and 18 are predominant in Yaounde, and are associated with more severe precancerous lesions.

scholarly journals Cervical Human Papillomavirus Prevalence, Genotypes, and Associated Risk Factors among Female Sex Workers in Greater Accra, Ghana

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Abdul Rashid Adams ◽  
Priscillia Awo Nortey ◽  
Benjamin Ansah Dortey ◽  
Richard Harry Asmah ◽  
Edwin Kwame Wiredu
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Cancer Prevention ◽  
Sex Workers ◽  
Female Sex Workers ◽  
Cervical Cancer Prevention ◽  
Human Papillomaviruses ◽  
Female Sex ◽  
Hpv Prevalence ◽  
Hpv Genotypes

Cervical cancer is a largely preventable disease mediated by persistent infection with high-risk Human Papillomaviruses (Hr-HPV). There are now three approved vaccines against the most common HPV genotypes. In Ghana, mortality due to cervical cancer is on the rise, due to the absence of an organized and effective cervical cancer prevention and control program. Data on circulating HPV genotypes is important for studying the likely impact of mass introduction of HPV vaccination of the female population before sexual debut. High HPV prevalence has been reported in Female Sex Workers (FSWs), who constitute an important active group for maintenance of HPV in the population. This study was conducted to determine the size of HPV prevalence in this group and to provide information for future assessment of the impact of vaccine introduction in the country. We conducted a cross-sectional study where the snowballing technique was used to identify and select FSW’s ≥18 years, operating within suburbs of Greater Accra Region (GAR). A risk factor assessment interview was conducted and cervical swabs were collected for HPV-DNA detection and genotyping by Nested Multiplex PCR. Hundred participants, age ranging from 18 to 45 years, median 24 years, were studied. The prevalence of Cervical HPV was 26%. Eleven genotypes were detected comprising 9 high-risk in order of decreasing prevalence HPV-16 (8%), HPV-35 (5%), HPV-33/39/-68 (3%), HPV-52/51/59 (2%) and HPV-18 (1%) and 2 Low-risk types, HPV-42(3%), and HPV-43 (1%). Three women had HPV types that could not be genotyped by our method. Oral contraceptives use was associated with a reduced chance of HPV infection (P=0.002; OR=0.19, 95% CI 0.07-0.54). This study found a high HPV prevalence among FSWs in the GAR. A high number of Hr-HPV genotypes seen are vaccine preventable, providing additional compelling argument for implementing a national cervical cancer prevention plan including vaccination.

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