scholarly journals Expression profiles of hsa-miR-148a-3p and hsa-miR-125b-5p in human breast milk and infant formulae

2022 ◽  
Vol 17 (1) ◽  
Author(s):  
Takeshi Chiba ◽  
Aya Kooka ◽  
Kiyoko Kowatari ◽  
Megumi Yoshizawa ◽  
Naoko Chiba ◽  
...  
Keyword(s):  
Breast Milk ◽  
Expression Profiles ◽  
Gastrointestinal Function ◽  
Human Breast Milk ◽  
Human Breast ◽  
Chain Reaction ◽  
Mature Milk ◽  
Infant Formulae ◽  
Polymerase Chain ◽  
Transition Milk

Abstract Background Milk-derived microRNAs (miRNAs), including hsa-miR-148a-3p (miR-148a) and hsa-miR-125b-5p (miR-125b), have been shown to be beneficial to the gastrointestinal function in infants. Here, we investigated their expression during lactation in humans and determined whether the infant formulae available in Japan contain these miRNAs. Methods Healthy Japanese women (n = 16) who gave birth vaginally or by cesarean section at the Teine Keijinkai Hospital between 1 September 2020, and 31 April 2021 were included in this study. Breast milk was collected by nurses on days 4 or 5 after delivery (hereinafter, transition milk) and on day 30 of postpartum (hereinafter, mature milk). The levels of miR-148a and miR-125b in breastmilk and six commercially available infant formulae were compared and evaluated using quantitative reverse transcription-polymerase chain reaction. Results In all participants, the miR-148a level in mature breastmilk was significantly lower than that in the transition milk. The changes in miR-125b expression during lactation showed similar trends to the changes in miR-148a expression. The miR-148a and miR-125b levels in all analyzed infant formulae were lower than 1/500th and 1/100th of those in mature breastmilk, respectively. Conclusions The levels of both miR-148a and miR-125b in human breast milk decreased on day 30 postpartum compared with those in the transition milk. Additionally, the expression of these miRNAs in infant formulae available in Japan was very low. Further studies with larger populations are required to understand precisely the lactational changes in the expression of miR148a and miR-125b in breast milk.

scholarly journals Polyamines in human breast milk for preterm and term infants

2013 ◽  
Vol 110 (3) ◽  
pp. 524-528 ◽  
Author(s):  
J. Plaza-Zamora ◽  
M. Sabater-Molina ◽  
M. Rodríguez-Palmero ◽  
M. Rivero ◽  
V. Bosch ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Human Breast Milk ◽  
Gut Development ◽  
Human Breast ◽  
Mature Milk ◽  
Term Infants ◽  
Milk Samples ◽  
Preterm Babies ◽  
Transition Milk

Maternal milk is the first source of exogenous polyamines for the newborn. Polyamines modulate gut maturation in neonates, but no studies are available on polyamine concentration in human milk of preterm babies, even though they could be important for their immature gut. The present study aimed to determine polyamine concentration in human breast milk of mothers with preterm or term infants during the first month of lactation. Human milk samples were obtained during the first month of lactation from twenty-seven mothers with preterm babies and twelve mothers with babies born at term. The polyamine concentration in human milk was quantified by HPLC. During the first month of lactation, the total polyamine concentration was significantly higher in preterm milk than in term milk samples (7590 (sd 4990) v. 4660 (sd 4830) nmol/l, respectively (P =0·034)), as well as individual polyamine concentrations. Polyamine concentration in mature milk for preterm babies was significantly higher than that in mature milk for babies at term, and a similar trend was observed in colostrum and transition human milk. The spermidine/spermine ratio was higher in transition milk in preterm v. term samples, while in mature milk, the ratio was significantly lower in preterm than in term babies. In conclusion, the polyamine concentration was significantly higher in human milk for preterm than for term infants. This and the different spermidine/spermine ratios could influence the gut development of premature babies.

scholarly journals 2586. Human Breast Milk Inhibits the Replication of Parechovirus-A3

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S899-S899
Author(s):  
Ryohei Izumita ◽  
Kazuki Kon ◽  
Yuta Aizawa ◽  
Kanako Watanabe ◽  
Akihiko Saitoh
Keyword(s):  
Breast Milk ◽  
Neutralizing Antibody ◽  
Antiviral Effect ◽  
Vero Cells ◽  
Human Breast Milk ◽  
Strong Positive Correlation ◽  
Human Breast ◽  
Mature Milk ◽  
Young Infants

Abstract Background Parechovirus-A3 (PeV-A3) is an emerging pathogen causing sepsis and meningoencephalitis in neonates and young infants. We previously reported that maternal antibodies against PeV-A3 are important to protect neonates and young infants from the infection. Recent studies showed that (1) breastfeeding had a protective effect against enterovirus, which is closely-related virus to PeV-A, and (2) human breast milk (HBM) neutralized enterovirus in vitro. Currently, no report is available related to the antiviral effect of HBM against PeV-A3. Methods HBM (colostrum, 3–5 days after childbirth; mature milk, 1 month after childbirth) and serum (within ± 1 week of child’s birthday) samples were obtained from mothers at obstetrics clinic in Niigata, Japan. Neutralizing antibody titers (NATs) against PeV-A3 were measured using the Vero cells. Results The anti-PeV-A3 NATs of colostrum (n = 32) ranged from 1:8 to 1:2048, those ≥1:32 was 59% (19/32). Whereas, the anti-PeV-A3 NATs of mature milk ranged from 1:8 to 1:96. and those ≥1:32 was 20% (2/20) (P < 0.001). The median NATs anti-PeV-A3 was higher in colostrum (1:32) compared with mature milk (1:8) (P < 0.001). There was a strong positive correlation between the NATs of colostrum and serum (r = 0.604, P < 0.001, Figure). Conclusion This study showed that HBM had high NATs against PeV-A3, which was correlated with serum NATs. Further studies are necessary to investigate which components of HBM has antiviral effects against PeV-A3. Disclosures All authors: No reported disclosures.

scholarly journals LACTOFERRIN LEVELS IN MOTHER’S MILK

2012 ◽  
Vol 19 (04) ◽  
pp. 527-530
Author(s):  
SYED QAISER HUSAIN NAQVI ◽  
MOHAMMAD SHIRAZ KHAN ◽  
ALI AKBAR SIYAL ◽  
Mir Muhammad Sehto ◽  
Riaz Ahmed Qazi ◽  
...  
Keyword(s):  
Breast Milk ◽  
Cross Sectional Study ◽  
Human Breast Milk ◽  
Sectional Study ◽  
Human Breast ◽  
Mature Milk ◽  
Cross Sectional ◽  
Lactating Mothers ◽  
Mother's Milk ◽  
The Mean

Objective: This study was aimed to see the significance of Lactoferrin in human breast milk among lactating mothers of healthyand sick babies. Place and duration: This study was conducted at pathology and paediatrics departments of Peoples University of Medical andHealth Sciences Nawabshah, Shaheed Benazirabad between Jan 2011 to Dec 2011. Design: Cross sectional study. Method: Lactoferrinlevels in breast milk of 356 mothers of healthy babies were estimated and similarly lactoferrin levels in breast milk of 318 lactating mothers ofsick babies were estimated & these results were analyzed. Results: the mean lactoferrin level in breast milk of 356 lactating mothers of healthybabies was 9.37 mg/ml and the mean lactoferrin level in breast milk of 318 mothers nursing sick babies was 3.73mg/ml. Conclusions: There isdecrease in lactoferrin levels of lactating mothers of sick babies in their mature milk, which could account for the susceptibility of their babies toinfection.

scholarly journals Perspective: The Role of Human Breast-Milk Extracellular Vesicles in Child Health and Disease

2020 ◽  
Author(s):  
Daniel O'Reilly ◽  
Denis Dorodnykh ◽  
Nina V Avdeenko ◽  
Nikita A Nekliudov ◽  
Johan Garssen ◽  
...  
Keyword(s):  
Breast Milk ◽  
Extracellular Vesicles ◽  
Basic Research ◽  
Normative Values ◽  
Future Research ◽  
Human Breast Milk ◽  
Human Breast ◽  
Mature Milk ◽  
Bioactive Substances ◽  
Beneficial Effects

ABSTRACT Human breast milk (HM) contains multiple bioactive substances determining its impact on children's health. Extracellular vesicles (EVs) are a heterogeneous group of secreted nanoparticles that are present in HM and may be partially responsible for its beneficial effects. The precise roles and content of EVs in HM remain largely unknown. To examine this, we performed a short narrative review on the literature focusing on HM EVs to contextualize the available data, followed by a scoping review of MEDLINE and Embase databases. We identified 424 nonduplicate citations with 19 original studies included. In this perspective, we summarize the evidence around HM EVs, highlight some theoretical considerations based on existing evidence, and provide an overview of some challenges associated with the complexity and heterogeneity of EV research. We consider how the existing data from HM studies conform to the minimal information for studies of EVs (MISEV) guidelines. Across the studies a variety of research methods were utilized involving both bench-based and translational methods, and a range of different EV contents were examined including RNA, proteins, and glycopeptides. We observed a variety of health outcomes in these studies, including allergy and atopy, necrotizing enterocolitis, and HIV. While some promising results have been demonstrated, the heterogeneity in outcomes of interest, methodological limitations, and relatively small number of studies in the field make comparison between studies or further translational work problematic. To date, no studies have examined normative values of HM EVs in a large, diverse population or with respect to potentially important influencing factors such as timing (hind- vs. foremilk), stage (colostrum vs. mature milk), and infant age (preterm vs. term), which makes extrapolation from bench or “basic” research impossible. Future research should focus on addressing the current inadequacies in the literature and utilize MISEV guidelines to inform study design.

scholarly journals Relation between breast milk energy density and the pre-conception BMI from donations of a human milk bank

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Yanira Flórez Matías ◽  
Liliana Ladino Melendez ◽  
Sandra Patricia Guevara Núñez
Keyword(s):  
Breast Milk ◽  
Energy Density ◽  
Human Milk ◽  
Milk Lipid ◽  
Human Breast ◽  
Mature Milk ◽  
Proportional Relation ◽  
Milk Bank ◽  
Transition Milk ◽  
Human Milk Bank

AbstractHuman breast milk is a high value biological substance that grants numerous benefits both to mother and child. Many aspects can produce changes in the milk composition; among them, the pre-conception body mass index (Pre-conception BMI) which is included as a factor that can influence the fat levels and therefore, the milk's energy density. To identify if there is a relation between the breast milk energy density from a human milk bank and Preconception BMI. It is a retrospective study that used information from donations registered in the human milk database at Hospital de Kennedy during 2016. The dependent variable is the energy density from 3 kinds of milk: colostrum, mature and transition milk. As independent variable, Preconception BMI was taken. The R free program version 3.3.0 was used for statistical analysis. The energy density average found for the 3 kinds of milk were: 20.59 + /- 2.3 kcal/oz, 20.51 + /- 2.17 kcal/oz y 19.21 + /- 2.53 for colostrum, transition milk and mature milk respectively. The Preconception BMI average from the donors was 23.56 Kg/m2 for colostrum, 23.9 Kg/m2 for transition milk and 23.1 Kg/m2 for mature milk. No relation between Preconception BMI and energy density of colostrum was found. However, for transition milk and Preconception BMI a directly proportional relation was found (p < 0.01), and for mature milk and Preconception BMI an inversely proportional relation was determined (p = 0.0387). There are internal and external factors than can affect the breast milk lipid content, such as movements of reserves through the organism to protect the milk production, hormonal action, dietary intake, physical activity and socio-economic and cultural aspects; that is why it is not possible to analyze the preconception BMI as a unique predictor of breast milk energy density in an isolated manner. Preconception BMI is related to human breast transition and mature milk energy density in donations registered in 2016 at Kennedy Hospital.

Human breast milk, infant formula, and follow-up milks comparison by electronic tongue

2021 ◽  
Vol 50 (1) ◽  
pp. 33-41
Author(s):  
M. Krpan ◽  
N. Major ◽  
Z. Šatalić ◽  
M. Hruškar
Keyword(s):  
Breast Milk ◽  
Infant Formula ◽  
Electronic Tongue ◽  
Human Breast Milk ◽  
Human Breast ◽  
Lactating Women ◽  
Future Studies ◽  
Infant Formulae ◽  
Breast Fed

AbstractHuman breast milk, infant formula, and follow-up milks were tested by a commercial electronic tongue (αAstree, Alpha MOS) with the aim to determine taste diversity, since it has been recently shown that infants exposed to different tastes early in life, develop different food preference at a later age. Human milk (36 samples) were obtained from 13 lactating women, while 12 samples of infant formula and 14 samples of follow-up milk were obtained from the Croatian market and opened prior to analysis. Human breast milk samples showed a much higher diversity than both infant formulae and follow-up milks. These results suggest that breast-fed infants are exposed to a broader sensory experience, while formula fed infants are exposed to less diverse taste. Future studies will probably answer how this influences later food choice, taste preferences, and consequently, risk of obesity and other chronic diseases.

scholarly journals Changes in HMO Concentrations throughout Lactation: Influencing Factors, Health Effects and Opportunities

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2272
Author(s):  
Caroline Thum ◽  
Clare Rosemary Wall ◽  
Gisela Adrienne Weiss ◽  
Wendan Wang ◽  
Ignatius Man-Yau Szeto ◽  
...  
Keyword(s):  
Breast Milk ◽  
Infant Health ◽  
Feeding Strategies ◽  
Human Breast Milk ◽  
Human Milk Oligosaccharides ◽  
Human Breast ◽  
Month Of Birth ◽  
Gradual Decline ◽  
Mature Milk ◽  
Factors Influencing

Human milk oligosaccharides (HMOs) are important functional biomolecules in human breast milk. Understanding the factors influencing differences in HMO composition and changes in their concentration over lactation can help to design feeding strategies that are well-adapted to infant’s needs. This review summarises the total and individual concentration of HMOs from data published from 1999 to 2019. Studies show that the HMO concentrations are highest in colostrum (average 9–22 g/L), followed by slightly lower concentrations in transitional milk (average 8–19 g/L), with a gradual decline in mature milk as lactation progresses, from 6–15 g/L in breast milk collected within one month of birth, to 4–6 g/L after 6 months. Significant differences in HMO composition have been described between countries. Different HMOs were shown to be predominant over the course of lactation, e.g., 3-fucosyllactose increased over lactation, whereas 2′-fucosyllactose decreased. Recent clinical studies on infant formula supplemented with 2′-fucosyllactose in combination with other oligosaccharides showed its limited beneficial effect on infant health.

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