Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solanacearum

Tomato (Solanum lycopersicum L.) is one of the most important vegetables in Togo. Unfortunately, tomatoes are susceptible to many diseases, among which bacterial wilt caused by Ralstonia solanacearum causes major yield losses. In this study, incidence of bacterial wilt and its distribution was evaluated in the central region of Togo, the major tomato producing area in the country. Overall, 16 localities were surveyed in four prefectures. In each locality, three fields were visited, and the incidence of the disease was recorded, and diseased samples were collected for laboratory investigation. The results showed that bacterial wilt occurred in all the fields visited, indicating a field incidence of 100%, whereas the plant incidence ranged from 10.00±00% to 43.33±3.33%, with an average of 20.94±1.77%. The antibody based Immunostrip test was positive for R. solanacearum in 100% of the visited fields. From 144 samples collected from fields, 45 R. solanacearum isolates were isolated on Modified SMSA media. This survey results show that tomato bacterial wilt is a real threat to tomato production in the central region of Togo.

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Characterization of plant growth promoting rhizobacteria and their potential as bioprotectant against tomato bacterial wilt caused by Ralstonia solanacearum

Biological Control ◽

10.1016/j.biocontrol.2013.07.004 ◽

2013 ◽

Vol 67 (1) ◽

pp. 75-83 ◽

Cited By ~ 29

Author(s):

Henok Kurabachew ◽

Kerstin Wydra

Keyword(s):

Plant Growth ◽

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Tomato Bacterial Wilt

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The potential of endomycorrhizal fungi in controlling tomato bacterial wilt Ralstonia solanacearum under glasshouse condition

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.3629 ◽

2012 ◽

Vol 11 (67) ◽

Author(s):

Monther Mohumad Tahat

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Glasshouse Condition ◽

Tomato Bacterial Wilt

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Effect of Allium fistulosum Extract on Ralstonia solanacearum Populations and Tomato Bacterial Wilt

Plant Disease ◽

10.1094/pdis-07-11-0601 ◽

2012 ◽

Vol 96 (5) ◽

pp. 687-692 ◽

Cited By ~ 20

Author(s):

Péninna Deberdt ◽

Benjamin Perrin ◽

Régine Coranson-Beaudu ◽

Pierre-François Duyck ◽

Emmanuel Wicker

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Management Strategies ◽

Antimicrobial Effect ◽

Allium Fistulosum ◽

Inhibition Assay ◽

In Vivo Experiments ◽

Tomato Bacterial Wilt

To control bacterial wilt (Ralstonia solanacearum, phylotype IIB/4NPB), the antimicrobial effect of Allium fistulosum aqueous extract was assessed as a preplant soil treatment. Three concentrations of extract (100, 50, and 25%, 1:1 [wt/vol]) were evaluated by in vitro inhibition assay and in vivo experiments in a growth chamber. In vitro, A. fistulosum (100 and 50%) suppressed growth of R. solanacearum. Preplant treatment of the soil with A. fistulosum extract significantly reduced the R. solanacearum populations. No pathogen was detected in the soil after treatment with 100% concentrated extract from the third day after application until the end of the experiment. A. fistulosum also significantly reduced the incidence of tomato bacterial wilt. In the untreated control, the disease affected 61% of the plants whereas, with 100 and 50% extracts, only 6 and 14% of the plants, respectively, were affected. These results suggest that A. fistulosum extracts could be used in biocontrol-based management strategies for bacterial wilt of tomato.

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Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex

BMC Microbiology ◽

10.1186/s12866-020-01774-y ◽

2020 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Ying Zhang ◽

Anna Hu ◽

Jianuan Zhou ◽

Wenfei Zhang ◽

Peng Li

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Bacterial Communities ◽

Species Complex ◽

Soil Samples ◽

Tomato Bacterial Wilt

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Combined use of a microbial restoration substrate and avirulent Ralstonia solanacearum for the control of tomato bacterial wilt

Scientific Reports ◽

10.1038/s41598-019-56572-y ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 7

Author(s):

Xuefang Zheng ◽

Yujing Zhu ◽

Jieping Wang ◽

Ziran Wang ◽

Bo Liu

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Combined Treatment ◽

Control Measures ◽

Effective Control ◽

Control Effect ◽

Tomato Production ◽

Combined Use ◽

Promote Plant Growth ◽

Tomato Bacterial Wilt

AbstractTomato bacterial wilt (BW) caused by Ralstonia solanacearum seriously restricts tomato production and no effective control measures are available. A microbial restoration substrate (MRS) had been proved to be effective control of tomato BW in a greenhouse cultivation. In this study, MRS was combined with an avirulent Ralstonia solanacearum (aRS) strain to control the disease under an open field condition. In the two consecutive year (2017 and 2018) trials, the combined use of aRS and MRS resulted in better disease control compared with either aRS or MRS alone. Moreover, the combined treatment was more effective than expected and suggesting a synergistic control effect. Compared with control (CK, non-aRS or MRS), the application of aRS and MRS treatments alone or in combination could all promote plant growth, increase root activity and yield (e.g. the yield for the treatment of aRS + MRS increased by 463.64% in 2017). Soil nutrients, including soil organic carbon, total nitrogen, total phosphorus and total potassium contents were also significantly increased by the application of aRS and MRS treatments alone or in combination (P < 0.05). The application of MRS or in combination with aRS changed the soil from acidic to neutral, which is one of the key factors for controlling BW. The soil enzymatic activities were notably influenced by the combined use of aRS and MRS, which increased urease (87.37% in 2017 and 60.89% in 2018), catalase (93.67% in 2017 and 279.37% in 2018) and alkaline phosphatase activities (193.77% in 2017 and 455.73% in 2018). These results suggest that the combination of MRS and aRS could effectively control tomato BW and thus represents a promising new tool to control this disease.

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