scholarly journals Ethyl acetate fraction of Cola hispida leaf protects against doxorubicin-induced myocardial injury in male albino rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chiemeka Lynda Umenwanne ◽  
Martins Obinna Ogugofor ◽  
Obioma U. Njoku
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Ethyl Acetate ◽  
Myocardial Injury ◽  
Ethyl Acetate Fraction ◽  
Albino Rats ◽  
Cardiac Biomarker ◽  
Novel Drugs ◽  
Myxomatous Degeneration ◽  
Pre Treatment

Abstract Background Cardiovascular diseases have continued to be the leading cause of death globally. In addition, some of the drugs used in the treatment of the diseases present some adverse effects which limit the usefulness of such drugs. Thus, there is a need for novel drugs whose side effect is either minimal or non-existent. The presence of bioactive compounds in Cola hispida leaf is of great significance in the treatment and management of cardiovascular conditions. This study investigated the cardio-protective potential against doxorubicin (Dox)-induced cardiac infarction in rats. Results Dox induction resulted to muscle fiber degeneration in Dox-treated rats hence revealed significant (p < 0.05) elevation in the serum level of cardio biomarker enzymes and lipid peroxidation profile while significant (p < 0.05) fall in cardiac enzymatic antioxidant levels were observed relative to the normal control. Pre-treatment with ethyl acetate fraction of Cola hispida leaf expressed cardio-protective potentials against Dox-induced cardiotoxicity by significantly (p < 0.05) lowering the levels of cardiac biomarker enzymes towards normal, building up the activities of subdued antioxidant enzymes and depleting its malondialdehyde level. Histopathology photomicrograph of the heart tissues expressed myxomatous degeneration but was ameliorated through the administration of the fraction. Conclusion In accordance with the findings from this study, the administration of ethyl acetate fraction of Cola hispida leaf is effective against Dox-induced redox imbalance due to its enriched antioxidant phytoconstituents.

Paraquat pre-treatment increases activities of antioxidant enzymes and reduces lipid peroxidation in salt-stressed cucumber leaves

2010 ◽  
Vol 33 (2) ◽  
pp. 295-304 ◽  
Author(s):  
Shao-Hang Lin ◽  
Zhong-Jing Liu ◽  
Pei-Lei Xu ◽  
Ye-Ying Fang ◽  
Ji-Gang Bai
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Pre Treatment

scholarly journals Lipid Peroxidation and Antioxidant Enzymes Evaluation in Lactating Female Albino Rats Following Supplementation with Fermented Soya Bean and Vitamin C

2017 ◽  
Vol 05 (02) ◽  
pp. 24-31
Author(s):  
I. S. Malgwi ◽  
Y. Tanko ◽  
M. U. Kawu ◽  
E. D. Eze ◽  
H. A. Salami ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Vitamin C ◽  
Soya Bean ◽  
Albino Rats

scholarly journals Cardioprotective Potential of Methanol Extract of Costus Afer Leaf on Carbon Tetrachloride-Induced Cardiotoxicity in Albino Rats

2017 ◽  
Vol 9 (2) ◽  
pp. 51 ◽  
Author(s):  
U. O. Njoku ◽  
O. F. C. Nwodo ◽  
M. O. Ogugofor
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Profile ◽  
Serum Lipid ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Density Lipoprotein ◽  
Albino Rats ◽  
Cardiac Biomarker ◽  
Costus Afer

<em>Costus afer</em> leaf extract is one of the native heritages of folklore medicine in Nigeria, used for the treatment of various disease conditions. Therefore this study was undertaken to evaluate the cardioprotective potential of the methanol extract of <em>Costus afer</em> leaves on CCl<sub>4</sub>- induced cardiotoxicity in albino rats. Twenty-eight (28) albino rats were divided into four main groups: normal control (normal saline only), CCl<sub>4</sub> control (saline and CCl<sub>4</sub>), aspirin treated (1.2 mg/kg b.w aspirin and CCl<sub>4</sub>) and <em>Costus afer</em> extract treated (extract and CCl<sub>4</sub>) groups. <em>Costus afer</em> extract was administered in four doses; 100, 200, 300, and 400 mg/kg b.w. p.o for 7 days. On day 7, CCl<sub>4</sub> (2.5 ml) cardiotoxicity was induced in animals of CCl<sub>4</sub> control, aspirin treated and extract pre-treatment groups. The parameters studied in this respect were cardiac biomarker enzymes; Creatine Kinase (CK), Aspartate Transaminases (AST) and Lactate Dehydrogenase (LDH), serum lipid profile; Triacylglycerol (TAG), Total Cholesterol (TC), Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL) and artherogenic index (AR), lipid peroxidation; malondialdehyde (MDA) level and enzymatic antioxidant levels of cardiac tissue homogenate; Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Reductase (GR). CCl<sub>4</sub> intoxication produced significant (p &lt; 0.05) increases in cardiac biomarker enzymes, serum lipid profile (TAG, TC, and LDL) along with enhanced lipid peroxidation in heart, while significant (p &lt; 0.05) decreases in enzymatic antioxidant activities and HDL contents were also observed when compared to normal control. <em>Costus afer</em> leaf extract protected against  CCl&lt; cardiotoxic effect by restoring the cardiac functions alterations, augmenting antioxidant activities, reducing lipid peroxidation and improving lipid profile levels near normal. From the results, <em>Costus afer</em> leaves expressed cardioprotective potential and could be helpful in the management of cardiac dysfunction.

EFFECT OF ETHANOLIC EXTRACT FRACTIONS OF ROSA CENTIFOLIA IN DOXORUBICIN-INDUCED MYOCARDIAL TOXICITY IN ALBINO RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (12) ◽  
pp. 27-33
Author(s):  
S. K Nimbal ◽  
◽  
B. C Koti
Keyword(s):  
Ethyl Acetate ◽  
Biochemical Parameters ◽  
Troponin I ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Repeated Administration ◽  
Albino Rats ◽  
Standard Drug ◽  
Preventive Role

This study was undertaken to understand the preventive role of ethanolic extract fractions of flower petals of Rosa centifolia (RC) against Doxorubicin-induced myocardial toxicity in albino rats. The ethanolic extract of flower petals of RC was prepared by hot extraction method and further fractionated into petroleum ether (PERC), chloroform (CHRC), ethyl acetate (EARC) and aqueous (AQRC) fractions by increasing in order of polarity. Cardiotoxicity was produced by cumulative administration of Dox (2.5 mg/kg alternative day for two weeks). All four fractions (PERC-20 mg/kg, CLRC-15 mg/kg, EARC-40 mg/kg and AQRC-25 mg/kg) were administered as pretreatment for two weeks followed by Dox on alternative days for two weeks. The general observations, biomarker enzymes like lactate dehydrogenase (LDH), creatine kinase (CK-MB) and troponin-I (cTnI), biochemical parameters such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were monitored after three weeks of last dose. Myocardial toxicity was also evaluated by histopathologic studies. Repeated administration of Dox-induced cardiomyopathy was characterized with an increased level of biomarkers and antioxidant deficit. Pretreatment with the EARC (40 mg/kg) and standard drug (Vit-E 100 mg/kg) significantly protected myocardium from the toxic effects of Dox by reducing the elevated level of biomarker enzymes like LDH, CK-MB, biochemical parameters such as AST and ALT, absence of cTnI and restoring of disorganized myocardial tissue to normal. The biomarker, biochemical and histopathological data evidently substantiate the cardioprotective effect of EARC, which could be attributed to flavanoids present in the ethyl acetate fraction.

HPLC-ESI-MS/MS phenolic profile of ‟Nanicão Corupá” (Musa acuminata)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Amanda Rodrigues Borges ◽  
Ana Paula Jungston Capistrano ◽  
Camila Saatkamp ◽  
Luisana Lusia Silveira Utzig ◽  
Bruna Gonçalves Lopes ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Musa Acuminata ◽  
Industrial Property ◽  
Esi Ms ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Pre Treatment ◽  
First Time

Abstract “Nanicão Corupá” (Musa acuminata) comes from Southern Brazil. The tropical climate in the region provides unique characteristics, including a sweeter flavor. This difference resulted in a Geographical Indication Recognition and Designation of Origin, recognized by the National Institute of Industrial Property (INPI) in Brazil. Considering that “Nanicão Corupá” has some peculiarities related to the climate and there are no studies evaluating this banana cultivars, the purpose of the present study was to investigate the qualitative and quantitative phenolic composition of the aerial parts of “Nanicão Corupá” by HPLC-ESI-MS/MS in comparison to 46 commercial standards of phenolic compounds. Aerial parts (flower, leaves, fruit and stem) of “Nanicão Corupá” were collected and macerated in methanolic extracts, which were partitioned with solvents of different polarities (dichloromethane and ethyl acetate). The HPLC-ESI-MS/MS analysis was performed using the sample pre-treatment, chromatographic and mass spectrometer parameters. Results demonstrated that a total of 11 phenolic compounds were identified in the analyzed samples. The majority of compounds was identified in the ethyl acetate fraction (BFEF) of banana flowers: rutin (36.06 ± 0.23) and isoquercetin (28.83 ± 5). The compounds isoquercetin, naringerin and myricitrin were identified for the first time in the Musa genus.

scholarly journals Evaluation of antioxidant, antimutagenic, and lipid peroxidation inhibitory activities of selected fractions of Holarrhena floribunda (G. Don) leaves.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Jelili A Badmus ◽  
Oyeronke A Odunola ◽  
Taofeek A Yekeen ◽  
Adedapo M Gbadegesin ◽  
John O Fatoki ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ethyl Acetate ◽  
Mitotic Index ◽  
Allium Cepa ◽  
Lipid Peroxides ◽  
Egg Yolk ◽  
Ethyl Acetate Fraction ◽  
Peroxyl Radicals ◽  
Antimutagenic Activity ◽  
Inhibitory Activities

Exposure to environmental pollutants often leads to an upsurge in the production of reactive oxygen species (ROS). ROS oxidize cellular fatty acids to produce lipid peroxyl radicals, subsequently transformed into lipid peroxides, which decrease membrane fluidity and increase the activity of various enzymes implicated in degenerative diseases and cancer formation. Edible plants that contain exogenous compounds like curcumeroid, β-carotene, turmeric, and so on, protect the aerobic cells from oxidation of free radicals. This study thus evaluates antioxidant and antimutagenic activities of ethyl acetate, aqueous and methanolic fractions of Holarrhena floribunda leaves. Inhibitory activities of the ethyl acetate fraction on Fe(2+)-induced lipid peroxidation in hen egg yolk; rat liver and brain tissues were also evaluated. The Allium cepa root assay was used to evaluate antimutagenic activity. Results showed that the ethyl acetate scavenged DPPH, OH•, and •O2(-) much stronger than other fractions, as evidenced by its lowest respective IC50 values. All the fractions displayed antimutagenic activities against cyclophosphamide-induced chromosomal aberrations. Likewise, all the fractions induced a reduction in mitotic index, a hallmark of cytotoxicity in the root meristem of Allium cepa. The decrease in mitotic index was most profound for the ethyl acetate fraction, which also demonstrated a significant lipid peroxidation inhibitory activity in the liver and brain homogenates, but not in egg yolk, compared with the ascorbic acid standard. In general, the results suggest that the ethyl acetate fraction might contain beneficial phytochemicals that should be explored as novel candidates for preclinical drug development.

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