Plant Roots Under Aluminum Stress

Elemental composition of living cells and tissues reflects their physiological function and status. However, it has been difficult to know in-situ elemental distribution by conventional analytical methods. In-air PIXE seems suitable for surface analysis of living cells and tissues because any treatment (e.g. freeze drying, digestion) is not required before and during measurement. We applied Via (vertical-beam in-air) PIXE to surface analysis of plant roots exposed to aluminum ( Al ). Aluminum stress is a major factor that limits elongation of plant roots in acid soils. We previously reported decrease in atomic ratio of potassium to phosphorus (K/P ratio) of dried root-tip of alfalfa (Medicago sativa L.) under Al stress using in-vacuum PIXE. In Via PIXE, 5 to 7-minute irradiation by 3 MeV proton beams of 200 pA was sufficient to obtain X-ray spectra without drying root samples. Decrease in K/P ratio in surface cells of root-tips was observed by short-term (6-8 h) exposure of root to Al . Via PIXE is recognized as a powerful tool for in-situ surface analysis of plant material.

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Plant Roots Under Aluminum Stress: Toxicity and Tolerance

Plant Roots ◽

10.1201/9780203909423-54 ◽

2002 ◽

pp. 1272-1297

Keyword(s):

Plant Roots ◽

Aluminum Stress

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Plant Roots under Aluminum Stress: Toxicity and Tolerance

Plant Roots ◽

10.1201/b14550-42 ◽

2013 ◽

pp. 548-571 ◽

Cited By ~ 12

Keyword(s):

Plant Roots ◽

Aluminum Stress

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Acute and chronic toxicity studies on Polygonum glabrum in experimental animals

International Journal of Pharmacometrics and Integrated Biosciences ◽

10.26452/ijpib.v3i1.1216 ◽

2020 ◽

Vol 3 (1) ◽

pp. 7-10

Author(s):

Saravanakumar A ◽

Gandhimathi R

Keyword(s):

Chronic Toxicity ◽

Clinical Stage ◽

Herbal Medicines ◽

Plant Roots ◽

Root Extract ◽

Experimental Animals ◽

Toxicity Studies ◽

Acute And Chronic Toxicity ◽

Diuretic Agents

Polygonum glabrum is being used in traditional and folklore medicine to treat pneumonia and jaundice. Plant roots are used in ayurvedic preparations to treat fever and colic. The leaves are used as diuretic agents and process vermifuge action. Plant decoction is also used in the treatment of Rheumatism. Besides having many uses and folklore claims, herbal medicines are to be thoroughly investigated for their toxicity also. Therefore this work is being carried out to examine the toxicity of the drug and established dose is safe to use in the clinical stage. The current research studied the acute and chronic toxicity of Polygonum glabrum root extract in rats. It is proved that there was no change in any parameter tested both in acute and chronic toxicity, which means the extract is safe and non-toxic at the dose of 2g/kg also.

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Effect of 24-epibrassinolide on localization of ABA, AZP, and dehydrins in wheat plant roots during dehydration

Biomics ◽

10.31301/2221-6197.bmcs.2020-20 ◽

2020 ◽

Vol 12 (3) ◽

pp. 329-336

Author(s):

A.R. Lubyanova ◽

F.M. Shakirova ◽

M.V. Bezrukova

Keyword(s):

Triticum Aestivum ◽

Wheat Germ ◽

Wheat Plant ◽

Triticum Aestivum L ◽

Immunohistochemical Localization ◽

Plant Roots ◽

Wheat Roots ◽

Wheat Seedlings ◽

Apoplastic Barrier ◽

Protective Proteins

We studied the immunohistochemical localization of abscisic acid (ABA), wheat germ agglutinin (WGA) and dehydrins in the roots of wheat seedlings (Triticum aestivum L.) during 24-epibrassinolide-pretreatment (EB-pretreatment) and PEG-induced dehydration. It was found coimmunolocalization of ABA, WGA and dehydrins in the cells of central cylinder of basal part untreated and EB-pretreated roots of wheat seedlings under normal conditions and under osmotic stress. Such mutual localization ABA and protective proteins, WGA and dehydrins, indicates the possible effect of their distribution in the tissues of EB-pretreated wheat roots during dehydration on the apoplastic barrier functioning, which apparently contributes to decrease the water loss under dehydration. Perhaps, the significant localization of ABA and wheat lectin in the metaxylem region enhances EB-induced transport of ABA and WGA from roots to shoots under stress. It can be assumed that brassinosteroids can serve as intermediates in the realization of the protective effect of WGA and wheat dehydrins during water deficit.

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