Transformations of Dibenzothiophene by Axenic Cultures of Sulfolobus acidocaldarius and Other Bacteria: A Critique

2018 ◽  
pp. 569-601
Author(s):  
Steven Krawiec
Keyword(s):  
Sulfolobus Acidocaldarius ◽  
Axenic Cultures

Peroxidase Localization in Hartmanella Trophozoites

1971 ◽  
Vol 29 ◽  
pp. 346-347 ◽  
Author(s):  
George E. Childs ◽  
Joseph H. Miller
Keyword(s):  
Hydrogen Peroxide ◽  
Ultrastructural Localization ◽  
Pathogenic Strain ◽  
Oxidative Enzymes ◽  
Differential Centrifugation ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
The Subject ◽  
Axenic Cultures

Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa. Cytochemical tests employing diaminobenzidine-tetra HCl (DAB) and hydrogen peroxide were used for the ultrastructural localization of peroxidases of trophozoites of Hartmanella sp. (A-l, Culbertson), a pathogenic strain grown in axenic cultures of trypticase soy broth.

UV Stimulation of Chromosomal Marker Exchange in Sulfolobus acidocaldarius: Implications for DNA Repair, Conjugation and Homologous Recombination at Extremely High Temperatures

Genetics ◽  
1999 ◽  
Vol 152 (4) ◽  
pp. 1407-1415 ◽  
Author(s):  
Katherine J Schmidt ◽  
Kristen E Beck ◽  
Dennis W Grogan
Keyword(s):  
Homologous Recombination ◽  
Incubation Temperature ◽  
Uv Light ◽  
Dna Lesions ◽  
Sulfolobus Acidocaldarius ◽  
Chromosomal Marker ◽  
Marker Exchange ◽  
Chromosomal Markers ◽  
Rate Of Decay ◽  
Uv Photoproducts

Abstract The hyperthermophilic archaeon Sulfolobus acidocaldarius exchanges and recombines chromosomal markers by a conjugational mechanism, and the overall yield of recombinants is greatly increased by previous exposure to UV light. This stimulation was studied in an effort to clarify its mechanism and that of marker exchange itself. A variety of experiments failed to identify a significant effect of UV irradiation on the frequency of cell pairing, indicating that subsequent steps are primarily affected, i.e., transfer of DNA between cells or homologous recombination. The UV-induced stimulation decayed rather quickly in parental cells during preincubation at 75°, and the rate of decay depended on the incubation temperature. Preincubation at 75° decreased the yield of recombinants neither from unirradiated parental cells nor from parental suspensions subsequently irradiated. We interpret these results as evidence that marker exchange is stimulated by recombinogenic DNA lesions formed as intermediates in the process of repairing UV photoproducts in the S. acidocaldarius chromosome.

The Structure and Evolution of the Ribosomal Proteins Encoded in the spc Operon of the Archaeon (Crenarchaeota) Sulfolobus acidocaldarius

1999 ◽  
Vol 12 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Decheng Yang ◽  
Isolde Kusser ◽  
Andreas K.E. Köpke ◽  
Ben F. Koop ◽  
Alastair T. Matheson
Keyword(s):  
Ribosomal Proteins ◽  
Sulfolobus Acidocaldarius

An efficient method to obtain axenic cultures of Alexandrium tamarense—a PSP-producing dinoflagellate

2007 ◽  
Vol 69 (3) ◽  
pp. 425-430 ◽  
Author(s):  
JianQiang Su ◽  
XiaoRu Yang ◽  
TianLing Zheng ◽  
HuaSheng Hong
Keyword(s):  
Efficient Method ◽  
Alexandrium Tamarense ◽  
Axenic Cultures

Production of axenic cultures of algae by an osmotic method

Phycologia ◽  
1982 ◽  
Vol 21 (3) ◽  
pp. 408-410 ◽  
Author(s):  
L. M. Brown
Keyword(s):  
Axenic Cultures

An infB-Homolog in Sulfolobus acidocaldarius

1996 ◽  
Vol 19 (3) ◽  
pp. 312-321 ◽  
Author(s):  
Patrick J. Keeling ◽  
Sandra L. Baldauf ◽  
W. Ford Doolittle ◽  
Wolfram Zillig ◽  
Hans-Peter Klenk
Keyword(s):  
Sulfolobus Acidocaldarius

Colony initiation in flax rust in axenic culture: involvement of a volatile factor

1979 ◽  
Vol 57 (23) ◽  
pp. 2657-2662 ◽  
Author(s):  
Rosalinda Boasson ◽  
Michael Shaw
Keyword(s):  
Carbon Dioxide ◽  
Axenic Culture ◽  
Conclusive Evidence ◽  
Culture Flask ◽  
Melampsora Lini ◽  
Air Space ◽  
Lag Period ◽  
Axenic Cultures ◽  
Incubation Temperatures

In axenic cultures of flax rust (Melampsora lini) colonies are initiated after a lag period of 12–20 days, depending partly on incubation temperatures. Colony initiation is completely inhibited by removal of a volatile factor which is absorbed by KOH in the air space of the culture flask. The fungus remains sensitive to this inhibition for 8–10 days, i.e., until shortly before visible colonies would normally have developed. While in the presence of KOH, the fungus is not killed; cultures grow normally after removal of the KOH.Although conclusive evidence must await further work, the available data strongly suggest that carbon dioxide is responsible for this effect.

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