Carbonic Anhydrase-Based Biosensing of Metal Ions: Issues and Future Prospects

2005 ◽  
pp. 125-138
Keyword(s):  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Future Prospects

scholarly journals Carbonic Anhydrase Inhibitors Part 721 Synthesis and Antiglaucoma Properties of Metal Complexes of p-Fluorobenzolamide

1999 ◽  
Vol 6 (2) ◽  
pp. 67-73 ◽  
Author(s):  
Claudiu T. Supuran ◽  
Andrea Scozzafava ◽  
Luca Menabuoni ◽  
Francesco Mincione ◽  
Fabrizio Briganti ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Metal Complexes ◽  
Carbonic Anhydrase Inhibitors ◽  
Physico Chemical ◽  
New Compounds

Metal complexes of a heterocyclic sulfonamides possessing very strong carbonic anhydrase (CA) inhibitory properties, i.e., 5-(p-fluorobenzenesulfonylamido)-1,3,4-thiadiazole-2-sulfonamide (p-fluorobenzolamide) were prepared. The new complexes contained metal ions such as Zn(II), Cu(II), Co(II), Ni(II), Cd(II) and Mn(II). The new compounds were characterized by standard physico-chemical procedures, and assayed as inhibitors of three CA isozymes, CA I, II and IV. Very good inhibition has been evidenced both for the parent sulfonamides as well as for the prepared complexes, against all three investigated isozymes. Some of these new complexes as well as the parent sulfonamide, strongly lowered intraocular pressure (IOP) in normotensive rabbits when administered as a 2% solution into the eye.

Carbonic Anhydrase-Based Biosensing of Metal Ions

2005 ◽  
pp. 107-119 ◽  
Author(s):  
Nissa Westerberg ◽  
Richard Thompson ◽  
Andrea Stoddard ◽  
Carol Fierke ◽  
Michele Cramer ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Metal Ions

scholarly journals Elucidating the role of metal ions in carbonic anhydrase catalysis

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Kyun Kim ◽  
Cheol Lee ◽  
Seon Woo Lim ◽  
Aniruddha Adhikari ◽  
Jacob T. Andring ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Metal Ion ◽  
Catalytic Mechanism ◽  
Chemical Properties ◽  
Water Network ◽  
Native Metal ◽  
Trigonal Bipyramidal ◽  
Electrostatic Effect

Abstract Why metalloenzymes often show dramatic changes in their catalytic activity when subjected to chemically similar but non-native metal substitutions is a long-standing puzzle. Here, we report on the catalytic roles of metal ions in a model metalloenzyme system, human carbonic anhydrase II (CA II). Through a comparative study on the intermediate states of the zinc-bound native CA II and non-native metal-substituted CA IIs, we demonstrate that the characteristic metal ion coordination geometries (tetrahedral for Zn2+, tetrahedral to octahedral conversion for Co2+, octahedral for Ni2+, and trigonal bipyramidal for Cu2+) directly modulate the catalytic efficacy. In addition, we reveal that the metal ions have a long-range (~10 Å) electrostatic effect on restructuring water network in the active site. Our study provides evidence that the metal ions in metalloenzymes have a crucial impact on the catalytic mechanism beyond their primary chemical properties.

scholarly journals Complexes With Biologically Active Ligands. Part 4. Coordination Compounds of Chlorothiazide With Transition Metal Ions Behave as Strong Carbonic Anhydrase Inhibitors

1996 ◽  
Vol 3 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Claudiu T. Supuran
Keyword(s):  
Transition Metal ◽  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Elemental Analysis ◽  
Coordination Compounds ◽  
Transition Metal Ions ◽  
Biologically Active ◽  
Carbonic Anhydrase Inhibitors

Complexes of the diuretic benzothiadiazine derivative chlorothiazide (6-chloro-7-sulfamoyl- 1,2,4-benzothiadiazine-1,1-dioxide) with V(IV); Fe(II); Co(II); Ni(II); Cu(II), Ag(I) and U(VI) were prepared and characterized by elemental analysis, spectroscopic, thermogravimetric, magnetic and conductimetric measurements. The complexes behave as effective inhibitors for two isozymes (I and II) of carbonic anhydrase (CA).

scholarly journals Complexes With Biologically Active Ligands. Part 111. Synthesis and Carbonic Anhydrase Inhibitory Activity of Metal Complexes of 4,5-Disubstituted-3-Mercapto-1,2,4-Triazole Derivatives

1998 ◽  
Vol 5 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Andrea Scozzafava ◽  
Christine Cavazza ◽  
Claudiu T. Supuran ◽  
Ioana Saramet ◽  
Fabrizio Briganti ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Nmr Spectroscopy ◽  
Animal Models ◽  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Metal Complexes ◽  
Elemental Analysis ◽  
Inhibitory Activity ◽  
Biologically Active ◽  
Standard Procedures

Complexes containing five 4,5-disubstituted-3-mercapto-1,2,4-triazoles and Zn(II), Hg(II) and Cu(I) were synthesized and characterized by standard procedures (elemental analysis; IR, electronic and NMR spectroscopy, conductimetry and TG analysis). Both the thione as well as the thiolate forms of the ligands were evidenced to interact with the metal ions in the prepared complexes. The original mercaptans and their metal complexes behave as inhibitors of three carbonic anhydrase (CA) isozymes, CA I, II and IV, but did not lower intraocular pressure in rabbits in animal models of glaucoma.

scholarly journals Complexes With Biologically Active Ligands. Part 1. Synthesis of Coordination Compounds of Diazoxide With Transition- and Main-Group Cations

1996 ◽  
Vol 3 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Claudiu T. Supuran
Keyword(s):  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Elemental Analysis ◽  
Coordination Compounds ◽  
Main Group ◽  
Pharmacological Agent ◽  
Biologically Active ◽  
Modest Activity

Complexes of diazoxide (3-methyl-7-chloro-1,2,4-benzothiadiazine-1,1-dioxide) - an antihypertensive and hyperglycemic pharmacological agent - with a series of transition- and main-group di-, triand tetravalent metal ions were prepared and characterized by elemental analysis, spectroscopic, thermogravimetric, magnetic and conductimetric measurements. The complexes were tested as inhibitors of the enzyme carbonic anhydrase (CA), proving modest activity towards CA II and better inhibition of CA I.

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