Oral Cancer Cells Differ From Normal Oral Epithelial Cells in Tissuelike Organization and in Response to Lycopene Treatment: An Organotypic Cell Culture Study

2003 ◽  
Vol 47 (2) ◽  
pp. 195-209 ◽  
Author(s):  
Orly Livny ◽  
Ilana Kaplan ◽  
Ram Reifen ◽  
Sylvie Polak-Charcon ◽  
Zecharia Madar ◽  
...  
Keyword(s):  
Cell Culture ◽  
Oral Cancer ◽  
Epithelial Cells ◽  
Cancer Cells ◽  
Culture Study ◽  
Cell Culture Study ◽  
Oral Epithelial Cells ◽  
Oral Cancer Cells ◽  
Oral Epithelial

MiR-195 Enhances the Sensitivity of Oral Cancer Cells to Cisplatin (DDP) by Down-Regulating the Expression of MAPK Kinase 1 (MEK1)

2020 ◽  
Vol 10 (2) ◽  
pp. 228-233
Author(s):  
Guoliang Niu ◽  
Meiling Yang ◽  
Shuai Tan ◽  
Hong Qian
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
Target Gene ◽  
Resistant Cell ◽  
Mapk Kinase ◽  
Oral Epithelial Cells ◽  
Oral Cancer Cells ◽  
Oral Epithelial ◽  
Proliferative Ability ◽  
Cancer Bioinformatics

MAPK kinase 1 (MEK1) is involved in the development and cisplatin (DDP) resistance of various tumors. Abnormal miR-195 expression is associated with oral cancer. Bioinformatics analysis revealed a relationship between miR-195 and MEK1. This study investigated whether miR-195 affects oral cancer cells through MEK1. The DDP-resistant cell OSCC-15/DDP was separated into miR-NC group and miR-195 mimic group followed by analysis of the expression of MEK1, p-MEK1 and p-ERK1/2 protein by Western blot, cell apoptosis and proliferation by flow cytometry. MEK1 mRNA is the target gene of miR-195. miR-195 was significantly lower in OSCC-15/DDP cells than OSCC-15 cells and human normal oral epithelial cells (HOEC) cells. MEK1 was significantly upreg-ulated in OSCC-15/DDP cells compared to OSCC-15 cells and HOEC cells. miR-195 mimic significantly elevated miR-195 in OSC-15/DDP cells, downregulated MEK1, p-MEK1 and p-ERK1/2 protein, promoted apoptosis and inhibited cell proliferative ability. miR-195 is related to oral cancer cell DDP resistance and upregulation of miR-195 down-regulates MEK1 expression and reduces DDP resistance.

Effect of Breast Milk Calcium and Fluidity on Breast Cancer Cells: An In Vitro Cell Culture Study

2016 ◽  
Vol 11 (9) ◽  
pp. 474-478 ◽  
Author(s):  
Recep Bayram ◽  
Muhsine Zeynep Yavuz ◽  
Bedri Selim Benek ◽  
Ayşenur Aydoğar Bozkurt ◽  
Ali Ucbek ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Breast Milk ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Culture Study ◽  
Cell Culture Study ◽  
In Vitro Cell Culture
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