scholarly journals Long-Term Treatment of Anterior Pituitary Cells with Nitric Oxide Induces Programmed Cell Death

Endocrinology ◽  
2004 ◽  
Vol 145 (4) ◽  
pp. 2064-2070 ◽  
Author(s):  
Miguel Omar Velardez ◽  
Ariel Hernán Poliandri ◽  
Jimena Paula Cabilla ◽  
Cristian Carlos Armando Bodo ◽  
Leticia Inés Machiavelli ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Anterior Pituitary ◽  
Term Treatment ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Long Term Treatment

Nitric oxide protects the mitochondria of anterior pituitary cells and prevents cadmium-induced cell death by reducing oxidative stress

2006 ◽  
Vol 40 (4) ◽  
pp. 679-688 ◽  
Author(s):  
Ariel H.B. Poliandri ◽  
Leticia I. Machiavelli ◽  
Alnilan F. Quinteros ◽  
Jimena P. Cabilla ◽  
Beatriz H. Duvilanski
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Cell Death ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells

scholarly journals Expression of Transgenes in Normal and Neoplastic Anterior Pituitary Cells Using Recombinant Adenoviruses: Long Term Expression, Cell Cycle Dependency, and Effects on Hormone Secretion*

Endocrinology ◽  
1997 ◽  
Vol 138 (5) ◽  
pp. 2184-2194 ◽  
Author(s):  
Maria G. Castro ◽  
Rodolfo G. Goya ◽  
Yolanda E. Sosa ◽  
Joanna Rowe ◽  
Adriana Larregina ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Anterior Pituitary ◽  
Hormone Secretion ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells

Long-term treatment with nipradilol, a nitric oxide–releasing β-adrenergic blocker, enhances postischemic recovery and limits infarct size

2002 ◽  
Vol 73 (1) ◽  
pp. 173-179 ◽  
Author(s):  
Yoshihiro Suematsu ◽  
Toshiya Ohtsuka ◽  
Hitoshi Horimoto ◽  
Katsuhide Maeda ◽  
Yasunari Nakai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Infarct Size ◽  
Term Treatment ◽  
Long Term Treatment ◽  
Adrenergic Blocker ◽  
Nitric Oxide Releasing ◽  
Postischemic Recovery

Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells

1998 ◽  
Vol 146 (1-2) ◽  
pp. 19-26 ◽  
Author(s):  
Beatriz H. Duvilanski ◽  
Miguel O. Velardez ◽  
Arturo Gonzalez Iglesias ◽  
Susana Theas ◽  
Adriana Seilicovich ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Intracellular Calcium ◽  
Anterior Pituitary ◽  
Nitric Oxide Donors ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Free Intracellular Calcium

Nitric oxide protects anterior pituitary cells from cadmium-induced apoptosis

2004 ◽  
Vol 37 (9) ◽  
pp. 1463-1471 ◽  
Author(s):  
Ariel H.B. Poliandri ◽  
Miguel O. Velardez ◽  
Jimena P. Cabilla ◽  
Cristian C.A. Bodo ◽  
Leticia I. Machiavelli ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Induced Apoptosis

scholarly journals Exocytotic protein components in rat pituitary gland after long-term estrogen administration

1999 ◽  
Vol 161 (2) ◽  
pp. 323-331 ◽  
Author(s):  
G Majo ◽  
MJ Lorenzo ◽  
J Blasi ◽  
F Aguado
Keyword(s):  
Pituitary Gland ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Fischer 344 Rats ◽  
Fischer 344 ◽  
Anterior Pituitary Cells ◽  
Pituitary Glands ◽  
Protein Components

Recently, a set of proteins involved in the docking and fusion machinery of secretory organelles has been identified in anterior pituitary cells. In this study we analyzed, by Western blotting and immunocytochemistry, the expression of several proteins involved in exocytosis after long-term administration of 17beta-estradiol (E2) in Fischer 344 rats. No differences were observed in the amount of synaptosomal-associated protein of 25 kDa, synaptobrevin 2, syntaxin 1, synaptotagmin I and Rab3a in total brain homogenates from treated rats after E2 administration. In striking contrast, the levels of all of these exocytotic proteins, including cellubrevin, were notably decreased in pituitary glands of E2-treated rats. In addition, no differences were observed in the in vitro basal and 8-Br-cAMP-induced prolactin (PRL) release between pituitary cells from control and E2-treated rats, whereas TRH-induced PRL release in anterior pituitary cells from E2-treated animals was higher than in control donors. In conclusion, this study shows that protein components of the exocytotic machinery are specifically down-regulated in the pituitary gland of E2-treated Fischer 344 rats.

scholarly journals Gonadotrophin storage patterns in the ewe during the oestrous cycle or after long-term treatment with a GnRH agonist

1998 ◽  
Vol 156 (1) ◽  
pp. 149-157 ◽  
Author(s):  
C Taragnat ◽  
A Bernier ◽  
J Fontaine
Keyword(s):  
Pituitary Gland ◽  
Gnrh Agonist ◽  
Term Treatment ◽  
Oestrous Cycle ◽  
Pituitary Cells ◽  
Double Immunostaining ◽  
Gonadotrophin Secretion ◽  
Long Term Treatment ◽  
Lh Cells

The storage pattern of gonadotrophins in the ewe pituitary was investigated during the oestrous cycle and after desensitization to GnRH using long-term treatment with a GnRH agonist, buserelin. Oestrous cycles in ewes were synchronized with progestagen sponges. Animals were allocated to two experiments. In the first, ewes were killed 36 h (before the preovulatory surge, n = 4), 48 h (end of the preovulatory surge, n = 5), 72 h (post-ovulation, n = 4) and 240 h (luteal phase, n = 3) after sponge removal. In the second experiment, another progestagen sponge was inserted in ewes 84 h after removal of the first sponge. Four ewes were infused continuously with buserelin (50 micrograms/day) for 15 days before killing. A further four ewes received no buserelin (controls). Pituitaries were collected and processed for immunocytochemistry to detect monohormonal (LH or FSH) and multihormonal (LH/FSH) cells. The percentages of LH or FSH immunoreactive cells in the pituitary were lower at the end of the preovulatory surge (7.4 +/- 0.3% and 1.2 +/- 0.3% respectively) compared with the other stages (11.4 +/- 0.5% and 5.4 +/- 0.7% respectively). Analysis of dual immunostaining showed the existence of monohormonal cells for LH and multihormonal cells (LH/FSH). No monohormonal cell for FSH was detected except at the end of the preovulatory surge when a few monohormonal FSH cells appeared (0.1 +/- 0.01% of pituitary cells). The percentage of monohormonal LH cells in the pituitary gland was similar in all studied stages of the oestrous cycle, whereas the percentage of multihormonal cells was lower at the end of the surge. In agonist-treated ewes, the percentages of LH or FSH immunoreactive cells (5.3 +/- 0.5% and 1.5 +/- 0.8% respectively) were decreased compared with controls (9.4 +/- 1% and 7.5 +/- 1.1% respectively). Analysis of the double immunostaining revealed a few monohormonal FSH cells (0.2 +/- 0.01% of pituitary cells) in agonist-treated ewes but not in controls. The percentage of monohormonal LH cells in the pituitary gland increased from 1.9 +/- 0.2% in controls to 3.8 +/- 0.3% in agonist-treated ewes, whereas multihormonal cells dropped from 7.5 +/- 1.1% to 1.3 +/- 0.7%. Our data suggest, therefore, that multihormonal cells contribute to gonadotrophin secretion, either during the preovulatory surge of the oestrous cycle or during the 'flare-up' effect initially induced by a GnRH agonist. Moreover, the appearance of monohormonal FSH cells in some conditions reflects a differential regulation of LH and FSH.

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