scholarly journals Mitochondria of Human Adrenal Cortex Have Tubular Cristae with Bulbous Tips

2003 ◽  
Vol 88 (4) ◽  
pp. 1903-1906 ◽  
Author(s):  
Alessandro Riva ◽  
Felice Loffredo ◽  
Alessandro Uccheddu ◽  
Francesca Testa Riva ◽  
Bernard Tandler
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Adrenal Cortex ◽  
Thin Sections ◽  
Transmission Electron ◽  
Soluble Material ◽  
Bulbous Tip ◽  
Scanning Electron

By taking advantage of a modified osmium maceration technique, we have been able to examine by high resolution scanning electron microscopy (HRSEM) the interior of human adrenocortical mitochondria from which all soluble material has been extracted. The so-called vesicles apparent in thin sections examined by transmission electron microscopy actually are finger-like cristae as determined by HRSEM. These digitiform cristae have a segmented appearance and a bulbous tip. The segmented form of the cristae may have important metabolic implications.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Radiation – induced preparation of polyaniline/poly vinyl alcohol nanocomposites and their properties

2019 ◽  
Vol 107 (8) ◽  
pp. 725-735
Author(s):  
Hoda H. Saleh ◽  
Rehab Sokary ◽  
Zakaria I. Ali
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Vinyl Alcohol ◽  
Oxidative Polymerization ◽  
Nanocomposite Films ◽  
Poly Vinyl Alcohol ◽  
Transmission Electron ◽  
Scanning Electron

Abstract Polyaniline (PANI) nanoparticles and PANI/poly vinyl alcohol (PVA) nanocomposite films were synthesized by the oxidative polymerization of aniline and ammonium peroxodisulfate (APS), as an oxidizing agent in aqueous medium. The PANI/PVA nanocomposite films were exposed to γ-irradiation after oxidative polymerization. Synthesized polyaniline (PANI) nanoparticles and PANI/PVA nanocomposite films were characterized by attenuated total reflectance infrared spectroscopy (FTIR-ATR), X-ray diffraction, high resolution scanning electron microscopy, (HRSEM) high resolution transmission electron microscopy, (HRTEM) and UV-VIS absorption spectroscopy. Energy band gap of PANI nanofibers was determined from Tauc’s plots which equal 4.2 eV. Scanning electron microscopy images show that chemically synthesized of polyaniline has nanofibers structure and irradiated PANI/PVA nanocomposite have a mixture of nanorod and nanosphere structures. The transmission electron microscopy show that chemically synthesized of polyaniline has average length in the range 34 ± 10 nm with less wide distribution, where as the irradiated PANI/PVA nanocomposite has coreshell structure.

scholarly journals A Rational Self-Sacrificing Template Route toLiMn2O4Nanotubes and Nanowires

2011 ◽  
Vol 2011 ◽  
pp. 1-5
Author(s):  
Baojun Yang ◽  
Xinsong Yuan ◽  
Duoli Chai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Spinel Phase ◽  
Synthesis Method ◽  
Molten Salt Synthesis ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Single-crystallineLiMn2O4nanotubes and nanowires have been synthesized via a low-temperature molten salt synthesis method, using the preparedβ-MnO2nanotubes andα-MnO2nanowires as the precursors and self-sacrificing template. The materials were investigated by a variety of techniques, including X-ray powder diffraction (XRD), transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). The results indicate that the preparedLiMn2O4nanotube and nanowire samples are both spinel phase, have lengths up to several micrometers and diameters of hundreds and tens of nanometers, respectively.

Image simulation in scanning electron microscopy

1987 ◽  
Vol 45 ◽  
pp. 74-75
Author(s):  
David C Joy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Image Interpretation ◽  
Image Simulation ◽  
Dimensional Metrology ◽  
Transmission Electron ◽  
Contrast Mechanisms ◽  
Scanning Electron

Image simulation, as a means of image interpretation, has been an important component of high resolution transmission electron microscopy for many years. By contrast scanning electron microscopists have relied almost exclusively on simple visual analogies to understand the micrographs that they produce. However with the development of SEMs capable of near atomic levels of resolution, and with the requirement for accurate dimensional metrology of sub-micron features in semiconductor devices using the SEM, a more quantitative approach to image formation and analysis is needed.Image simulation in the SEM encounters several special problems. Firstly, the signal displayed to form the micrograph is not well defined in terms of either the contrast mechanisms which give rise to it, or the energy and origin of the electrons actually collected to produce the signal.

scholarly journals Colloidal gold, a useful marker for transmission and scanning electron microscopy.

1977 ◽  
Vol 25 (4) ◽  
pp. 295-305 ◽  
Author(s):  
M Horisberger ◽  
J Rosset
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Yeast Cells ◽  
Erythrocyte Membranes ◽  
Optimum Conditions ◽  
Thin Sections ◽  
Good Spatial Resolution ◽  
Transmission Electron ◽  
Scanning Electron

Electron dense markers of a size suitable for transmission electron microscopy and scanning electron microscopy have been prepared with gold granules labeled with a monolayer of specific macromolecules. The optimum conditions for preparing the markers have been ascertained. The method is simple, rapid and seems to be general since gold granules have been labeled with polysaccharides and proteins. As homogeneous populations of gold granules having different sizes can be prepared, the method is also suitable for double marking experiments. The gold technique is illustrated by the localization of polysaccharides and glycoproteins on yeast cell walls and erythrocyte membranes by transmission electron microscopy and on yeast cells and intact erythrocytes by scanning electron microscopy. Good spatial resolution of the marker was achieved in all cases. The method is also suitable for marking thin sections. Spectrophotometric measurements were used to determine the number of gold granules adsorbed per cell.

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