scholarly journals Comparison of Early (2 Days) and Later (28 Days) Response of Adipose Tissue to Very Low-Calorie Diet in Obese Women

2016 ◽  
Vol 101 (12) ◽  
pp. 5021-5029 ◽  
Author(s):  
Veronika Šrámková ◽  
Lenka Rossmeislová ◽  
Eva Krauzová ◽  
Jana Kračmerová ◽  
Michal Koc ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Obese Women ◽  
Very Low Calorie Diet ◽  
Low Calorie ◽  
Low Calorie Diet ◽  
Calorie Diet

scholarly journals Expression of Inflammation-Related Genes in Gluteal and Abdominal Subcutaneous Adipose Tissue During Weight-Reducing Dietary Intervention in Obese Women

2014 ◽  
pp. 73-82 ◽  
Author(s):  
L. MALIŠOVÁ ◽  
L. ROSSMEISLOVÁ ◽  
Z. KOVÁČOVÁ ◽  
J. KRAČMEROVÁ ◽  
M. TENCEROVÁ ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Dietary Intervention ◽  
Weight Maintenance ◽  
Maintenance Phase ◽  
Obese Women ◽  
Very Low Calorie Diet ◽  
Low Calorie ◽  
Paired Samples ◽  
Low Calorie Diet ◽  
Calorie Diet

Accumulation of adipose tissue in lower body lowers risk of cardiovascular and metabolic disorders. The molecular basis of this protective effect of gluteofemoral depot is not clear. The aim of this study was to compare the profile of expression of inflammation-related genes in subcutaneous gluteal (sGAT) and abdominal (sAAT) adipose tissue at baseline and in response to multiphase weight-reducing dietary intervention (DI). 14 premenopausal healthy obese women underwent a 6 months’ DI consisting of 1 month very-low-calorie-diet (VLCD), subsequent 2 months’ low-calorie-diet and 3 months’ weight maintenance diet (WM). Paired samples of sGAT and sAAT were obtained before and at the end of VLCD and WM periods. mRNA expression of 17 genes (macrophage markers, cytokines) was measured using RT-qPCR on chip-platform. At baseline, there were no differences in gene expression of macrophage markers and cytokines between sGAT and sAAT. The dynamic changes induced by DI were similar in both depots for all genes except for three cytokines (IL6, IL10, CCL2) that differed in their response during weight maintenance phase. The results show that, in obese women, there are no major differences between sGAT and sAAT in expression of inflammation-related genes at baseline conditions and in response to the weight-reducing DI.

scholarly journals Expression of Lipolytic Genes in Adipose Tissue Is Differentially Regulated During Multiple Phases of Dietary Intervention in Obese Women

2013 ◽  
pp. 527-535 ◽  
Author(s):  
K. KOPPO ◽  
C. VALLE ◽  
M. ŠIKLOVÁ-VÍTKOVÁ ◽  
E. CZUDKOVÁ ◽  
I. DE GLISEZINSKI ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Time Course ◽  
Dietary Intervention ◽  
Hormone Sensitive Lipase ◽  
Mrna Levels ◽  
Obese Women ◽  
Very Low Calorie Diet ◽  
Low Calorie ◽  
Low Calorie Diet ◽  
Calorie Diet

The aim of this study was to investigate the time-course of the expression of key lipolysis-regulating genes in the subcutaneous adipose tissue (SCAT) during different phases of a 6-month dietary intervention. Fifteen obese women (BMI 34.7±1.0 kg.m-2) underwent a 6-month dietary intervention consisting of 1 month very low calorie diet (VLCD), followed by 2 months low calorie diet (LCD) and 3 months weight maintenance diet (WM). At each phase of the dietary intervention, a needle microbiopsy of the abdominal SCAT was obtained to evaluate mRNA expression of key lipolysis-regulating genes and a hyperinsulinemic euglycemic clamp (HEC) was performed. Dietary intervention induced a body weight reduction of 9.8 % and an improvement of insulin sensitivity as assessed by a HEC. Compared to pre-diet levels, mRNA levels of the adrenergic β2-receptor in SCAT were higher at the end of VLCD and not different at the end of LCD and WM. In contrast, the expression of the adrenergic α2-receptor was lower at the end of VLCD and LCD compared to the pre-diet levels and did not differ at WM. Adipose triglyceride lipase and hormone-sensitive lipase levels were lower than the pre-diet levels at the end of LCD only, while phosphodiesterase-3B and the insulin receptor levels did not change throughout the dietary intervention. The results suggest that the regulation pattern of the genes that are involved in the control of lipolysis is different at the respective phases of the dietary intervention and depends on the duration of the diet and the status of energy balance.

scholarly journals Lipoprotein Metabolism in Normolipidemic Obese Women during Very Low Calorie Diet

1991 ◽  
Vol 37 (Supplement) ◽  
pp. S57-S64 ◽  
Author(s):  
Tetsuo SHOJI ◽  
Yoshiki NISHIZAWA ◽  
Hidenori KOYAMA ◽  
Satoshi HAGIWARA ◽  
Hideyuki ARATANI ◽  
...  
Keyword(s):  
Lipoprotein Metabolism ◽  
Obese Women ◽  
Very Low Calorie Diet ◽  
Low Calorie ◽  
Low Calorie Diet ◽  
Calorie Diet

Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women

2012 ◽  
Vol 302 (2) ◽  
pp. E226-E232 ◽  
Author(s):  
Katrien Koppo ◽  
Michaela Siklová-Vitková ◽  
Eva Klimčáková ◽  
Jan Polák ◽  
Marie A. Marques ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Subcutaneous Adipose Tissue ◽  
Dietary Intervention ◽  
Glycerol Concentration ◽  
Obese Women ◽  
Low Calorie ◽  
Low Calorie Diet ◽  
Calorie Diet ◽  
Subcutaneous Adipose

The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α2-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.

scholarly journals Increased serum concentrations of macrophage inhibitory cytokine-1 in patients with obesity and type 2 diabetes mellitus: the influence of very low calorie diet

2009 ◽  
Vol 161 (3) ◽  
pp. 397-404 ◽  
Author(s):  
Ivana Dostálová ◽  
Tomáš Roubíček ◽  
Markéta Bártlová ◽  
Miloš Mráz ◽  
Zdena Lacinová ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Mrna Expression ◽  
Obese Women ◽  
Very Low Calorie Diet ◽  
Low Calorie ◽  
Low Calorie Diet ◽  
Calorie Diet

ObjectiveMacrophage inhibitory cytokine-1 (MIC-1) is a novel regulator of energy homeostasis. We explored whether alterations in MIC-1 levels contribute to metabolic disturbances in patients with obesity and/or obesity and type 2 diabetes mellitus (T2DM).DesignWe measured serum MIC-1 levels and its mRNA expression in subcutaneous and visceral adipose tissue of 17 obese nondiabetic women, 14 obese women with T2DM and 23 healthy lean women. We also explored the relationship of MIC-1 with anthropometric and biochemical parameters and studied the influence of 2-week very low calorie diet (VLCD) on serum MIC-1 levels.MethodsSerum MIC-1 levels were measured by ELISA and its mRNA expression was determined by RT-PCR.ResultsBoth obese and T2DM group had significantly elevated serum MIC-1 levels relative to controls. T2DM group had significantly higher serum MIC-1 levels relative to obese group. Serum MIC-1 positively correlated with body weight, body fat, and serum levels of triglycerides, glucose, HbAlc, and C-reactive protein and it was inversely related to serum high-density lipoprotein cholesterol. Fat mRNA MIC-1 expression did not significantly differ between lean and obese women but it was significantly higher in subcutaneous than in visceral fat in both groups. VLCD significantly increased serum MIC-1 levels in obese but not T2DM group.ConclusionElevated MIC-1 levels in patients with obesity are further increased by the presence of T2DM. We suggest that in contrast to patients with cancer cachexia, increased MIC-1 levels in obese patients and diabetic patients do not induce weight loss.

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