Estrone Sulfate Sulfatase Activity Is Increased duringin VitroDecidualization of Stromal Cells from Human Endometrium*

1990 ◽  
Vol 70 (2) ◽  
pp. 342-345 ◽  
Author(s):  
M. T. BENEDETTO ◽  
S. TABANELLI ◽  
E. GURPIDE
Keyword(s):  
Stromal Cells ◽  
Human Endometrium ◽  
Estrone Sulfate ◽  
Sulfatase Activity

Regulation of estrogen activity in human endometrium: effect of IL-1β on steroid sulfatase activity in human endometrial stromal cells

Steroids ◽  
2002 ◽  
Vol 67 (7) ◽  
pp. 655-659 ◽  
Author(s):  
Ryu Matsuoka ◽  
Atsushi Yanaihara ◽  
Hiroshi Saito ◽  
Yoshiaki Furusawa ◽  
Yoshiro Toma ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Human Endometrium ◽  
Steroid Sulfatase ◽  
Endometrial Stromal Cells ◽  
Estrogen Activity ◽  
Sulfatase Activity

Focal cellular origin and regulation of interstitial collagenase (matrix metalloproteinase-1) are related to menstrual breakdown in the human endometrium

1996 ◽  
Vol 109 (8) ◽  
pp. 2151-2160 ◽  
Author(s):  
I. Kokorine ◽  
E. Marbaix ◽  
P. Henriet ◽  
Y. Okada ◽  
J. Donnez ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Steroid Receptors ◽  
Progesterone Receptors ◽  
Human Endometrium ◽  
Functional Layer ◽  
Cellular Origin ◽  
Matrix Metalloproteinase 1 ◽  
Essential Enzyme ◽  
Interstitial Collagenase

Recent studies suggest that interstitial collagenase (MMP-1) is an essential enzyme in the early events leading to menstruation. This study analyses its cellular origin, regulation and relation to extracellular matrix breakdown in the human endometrium, both in cultured and non-cultured samples. The source of MMP-1 was identified by in situ hybridization and by immunohistochemistry on serial sections. This was compared with the immunolocalization of other MMPs, steroid receptors, macrophages, and laminin. In non-cultured endometrium, MMP-1 was only expressed during the perimenstrual period. It was either restricted to superficial foci of stromal cells or extended towards the entire functional layer. MMP-1 expression remarkably correlated with matrix breakdown, as assessed by silver staining, and was prominent at the periphery of shedding fragments and along some arterioles. In cultured non-menstrual explants, MMP-1 expression was induced within two days after deprivation of sex steroids. Both in cultured and non-cultured samples, progesterone receptors were not detectable in epithelial cells at foci of MMP-1 expression. The same stromal cells could synthesize MMP-1, MMP-2 (gelatinase A) and MMP-3 (stromelysin-1), as well as laminin, and did not correspond to macrophages. In conclusion, MMP-1 is focally expressed in stromal cells of the functional layer of the endometrium, when and where steroid receptors disappear, and especially where tissue breakdown is prominent. These observations point to an essential role for MMP-1 in the early stages of menstruation.

scholarly journals An improved method for isolation of epithelial and stromal cells from the human endometrium

2016 ◽  
Vol 62 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Ayako MASUDA ◽  
Noriko KATOH ◽  
Kazuhiko NAKABAYASHI ◽  
Kiyoko KATO ◽  
Kenzo SONODA ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Human Endometrium ◽  
Improved Method

Intercellular contacts between stromal cells in the normal human endometrium throughout the menstrual cycle

1990 ◽  
Vol 21 (10) ◽  
pp. 1063-1066 ◽  
Author(s):  
T.H. Parmley ◽  
D.K. Roberts ◽  
N.J. Walker ◽  
D.V. Horbelt
Keyword(s):  
Menstrual Cycle ◽  
Stromal Cells ◽  
Human Endometrium ◽  
Intercellular Contacts ◽  
Normal Human

scholarly journals Characteristics of the human endometrial regeneration cells as a potential source for future stem cell-based therapies: A lab resources study

2020 ◽  
Author(s):  
Fatemeh Akyash ◽  
Mahdieh Javidpou ◽  
Ehsan Farashahi Yazd ◽  
Jalal Golzadeh ◽  
Fatemeh Hajizadeh-Tafti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stromal Cells ◽  
Human Endometrium ◽  
Hormonal Changes ◽  
Alizarin Red ◽  
Differentiation Capacity ◽  
Endometrial Cells ◽  
Cell Based Therapy ◽  
Regeneration Capability

Background: Human endometrium with consecutive regeneration capability undergoes monthly hormonal changes for probable implantation, which confirms the presence of the cells in the basalis layer known as stem cell. Objective: Previously, we reported the isolation and culture of the mesenchymal-like cells from human endometrium. In this study, we evaluated the biological and stemness characteristics of these cells. Materials and Methods: The characterization of Yazd human endometrialderived mesenchymal stem/stromal cells (YhEnMSCs) was assessed using immunofluorescence (IF) staining for CD105, VIMENTIN, and FIBRONECTIN as markers and RT-PCR for CD166, CD10, CD105, VIMENTIN, FIBRONECTIN, MHCI, CD14, and MHCII genes. Flow cytometry (FACS) was performed for CD44, CD73, CD90, and CD105 markers. Moreover, the differentiation capacity of the YhEnMSCs to the osteoblast and adipocytes was confirmed by Alizarin Red and Oil Red staining. Results: YhEnMSCs expressed CD105, VIMENTIN, FIBRONECTIN, CD44, CD73, and CD90 markers and CD166, CD10, CD105, VIMENTIN, FIBRONECTIN, and MHCI, but, did not express CD14, MHCII. Conclusion: Our data confirm previous reports by other groups indicating the application of endometrial cells as an available source of MSCs with self-renewal and differentiation capacity. Accordingly, YhEnMSCs can be used as a suitable source for cell-based therapies. Key words: Cell-based therapy, Endometrium, Mesenchymal stem/stromal cells, Regenerative medicine, Stem cells, Uterus.

240. Spatial and temporal expression pattern of furin in the human endometrium

2005 ◽  
Vol 17 (9) ◽  
pp. 95
Author(s):  
C. Freyer ◽  
L. Kilpatrick ◽  
L. Salamonsen ◽  
G. Nie
Keyword(s):  
Menstrual Cycle ◽  
Expression Pattern ◽  
Early Pregnancy ◽  
Stromal Cells ◽  
Human Endometrium ◽  
Mrna Levels ◽  
Temporal Expression ◽  
Proliferative Phase ◽  
Membrane Bound ◽  
Temporal Expression Pattern

Furin is a proprotein convertase (PC) implicated in the endoproteolytic maturation of inactive protein precursors of growth factors, hormones, receptors, and viral envelope glycoproteins.1 Two functionally active forms of furin, one membrane-bound containing a C-terminal transmembrane domain (TD) and a cytoplasmic tail (CT), and one soluble without the TD and CT, have been characterised. We have previously shown that PC6, one of the PCs closely related to furin, is expressed in the human endometrium and is closely associated with decidualization of stromal cells during implantation.2 Although furin is ubiquitously expressed, its expression in the human endometrium is unknown. In this study, we investigated the spatial and temporal expression pattern of furin in the human endometrium using RT-PCR and immunohistochemistry. While furin expression is detected throughout the menstrual cycle and during early pregnancy, lowest mRNA levels are seen during the proliferative phase. Using an antibody directed against the C-terminus of the membrane bound form, furin is detected in the stroma, glandular and luminal epithelium, as well as in endothelia and neutrophils throughout the menstrual cycle and during early pregnancy. In the stroma, highest levels of furin are present during menstruation (n = 3), they are also high during the proliferative phase (n = 4), but significantly lower levels are detected during the secretory phase (n = 10, P < 0.05, Tukey HSD). In the first trimester decidua, furin is present in well decidualised stromal cells. The overall expression pattern of furin is different to that of PC6; in particular, furin expression is associated only with well decidualized stromal cells whereas PC6 is involved in the initial stages of decidualization. These data suggest that furin and PC6 play different roles in the human endometrium, especially during embryo implantation. (1)Nakayama K. (1997). Biochem. J. 327, 625–635.(2)Nie et al. (2005). Biol. Reprod. 72, 1029–1036.

271. Prospective isolation of human endometrial mesenchymal stem cells using CD146 and platelet-derived growth factor receptor-β

2005 ◽  
Vol 17 (9) ◽  
pp. 111
Author(s):  
K. E. Schwab ◽  
C. E. Gargett
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Stromal Cells ◽  
Growth Factor Receptor ◽  
Small Population ◽  
Human Endometrium ◽  
Platelet Derived Growth Factor ◽  
Endometrial Stromal Cells ◽  
Prospective Isolation

The human endometrium has an immense regenerative capacity. Previously we identified a small population of clonogenic endometrial stromal cells or mesenchymal stem cells (MSC).1 Prospective isolation of MSC allows for their characterisation. We hypothesise that the expression of MSC marker, CD146, and pericyte/fibroblast marker, platelet-derived growth factor receptor-β (PDGFRβ), will enable the prospective isolation of endometrial MSCs. The aims of this study were to (1) determine if CD146 and PDGFRβ will prospectively isolate endometrial MSCs with clonogenic activity, (2) identify their location in human endometrium, and (3) determine the differentiation capacity of CD146+PDGFRβ+ stromal cells. Endometrial tissue from 13 ovulating women undergoing hysterectomy was digested with collagenase to produce single cell suspensions. Leukocytes and epithelial cells were removed. Stromal cells were analysed by flow cytometry, FACS sorted into enriched and depleted populations, and cultured for clonal analysis.1 Immunohistochemistry was performed on full thickness human endometrium. Sorted populations of stromal cells were passaged for culture in various differentiation media, and analysed for adipogenic, myogenic, chondrogenic or osteogenic differentiation by histological stains and RT-PCR. A small, consistent population of CD146+ endometrial stromal cells was identified (7.8 ± 1.1%, n = 8). In contrast, PDGFRβ expression varied (34.1 ± 9.7%, n = 5), and 2.5% of cells were CD146+PDGFRβ+. Clonogenicity of CD146+ stromal cells was significantly higher than CD146- stromal cells, 2.5 ± 1.1% and 1.2 ± 0.6%, respectively (n = 6, P = 0.03). CD146+ stromal cells were located perivascularly, similar to bone marrow MSCs, whereas PDGFRβ weakly stained the stroma, with stronger staining observed around the blood vessels. CD146+ cells differentiated into adipocytes, smooth muscle cells, chondrocytes and osteoblasts. This study identified CD146 as a marker of clonogenic endometrial stromal cells, and supports the perivascular location of endometrial MSCs. It also demonstrated that CD146+ cells can differentiate into four mesenchymal lineages. These data suggest that CD146 can be used for the prospective isolation of endometrial MSCs, which may be further enriched by PDGFRβ co-expression. (1)Chan RW, Schwab KE and Gargett CE (2004) Biology of Reproduction 70, 1738.

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