Determination of aromatase cytochrome P450 messenger ribonucleic acid in human breast tissue by competitive polymerase chain reaction amplification.

1992 ◽  
Vol 74 (6) ◽  
pp. 1247-1252
Author(s):  
T Price ◽  
J Aitken ◽  
J Head ◽  
M Mahendroo ◽  
G Means ◽  
...  
Keyword(s):  
Breast Tissue ◽  
Polymerase Chain Reaction Amplification ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Messenger Ribonucleic Acid ◽  
Reaction Amplification ◽  
Polymerase Chain

scholarly journals DNA Amplified Fingerprinting, A Useful Tool for Determination of Genetic Origin and Diversity Analysis in Citrus

HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1063-1067 ◽  
Author(s):  
François Luro ◽  
Frédéric Laigret ◽  
Joseph-Marie Bové ◽  
Patrick Ollitrault
Keyword(s):  
Polymerase Chain Reaction Amplification ◽  
Hybrid Plant ◽  
Chain Reaction ◽  
Genetic Origin ◽  
Volkamer Lemon ◽  
Citrus Volkameriana ◽  
Reaction Amplification ◽  
Successful Technique ◽  
Polymerase Chain

We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related.

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