scholarly journals A Bayesian nonparametric approach to super-resolution single-molecule localization

2021 ◽  
Vol 15 (4) ◽  
Author(s):  
Mariano I. Gabitto ◽  
Herve Marie-Nelly ◽  
Ari Pakman ◽  
Andras Pataki ◽  
Xavier Darzacq ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Bayesian Nonparametric ◽  
Nonparametric Approach ◽  
Resolution Single

scholarly journals A Bayesian Nonparametric Approach to Image Super-Resolution

2015 ◽  
Vol 37 (2) ◽  
pp. 346-358 ◽  
Author(s):  
Gungor Polatkan ◽  
Mingyuan Zhou ◽  
Lawrence Carin ◽  
David Blei ◽  
Ingrid Daubechies
Keyword(s):  
Super Resolution ◽  
Bayesian Nonparametric ◽  
Nonparametric Approach ◽  
Image Super Resolution

Revealing dynamically-organized receptor ion channel clusters in live cells by a correlated electric recording and super-resolution single-molecule imaging approach

2018 ◽  
Vol 20 (12) ◽  
pp. 8088-8098 ◽  
Author(s):  
Rajeev Yadav ◽  
H. Peter Lu
Keyword(s):  
Ion Channel ◽  
Single Molecule ◽  
Super Resolution ◽  
Live Cells ◽  
Imaging Approach ◽  
Resolution Imaging ◽  
Function Relationship ◽  
Bleaching Step ◽  
Relationship Of ◽  
Resolution Single

Correlating single-molecule fluorescence photo-bleaching step analysis and single-molecule super-resolution imaging, our findings for the clustering effect of the NMDA receptor ion channel on the live cell membranes provide a new and significant understanding of the structure–function relationship of NMDA receptors.

scholarly journals Deep-STORM: super-resolution single-molecule microscopy by deep learning

Optica ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 458 ◽  
Author(s):  
Elias Nehme ◽  
Lucien E. Weiss ◽  
Tomer Michaeli ◽  
Yoav Shechtman
Keyword(s):  
Deep Learning ◽  
Single Molecule ◽  
Super Resolution ◽  
Single Molecule Microscopy ◽  
Resolution Single

scholarly journals Defining the Basis of Cyanine Phototruncation Enables a New Approach to Single Molecule Localization Microscopy

2021 ◽  
Author(s):  
Siddharth Matikonda ◽  
Dominic Helmerich ◽  
Mara Meub ◽  
Gerti Beliu ◽  
Philip Kollmannsberger ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Single Molecule ◽  
Computational Analysis ◽  
Super Resolution ◽  
Underlying Mechanism ◽  
New Approach ◽  
Localization Microscopy ◽  
Order Of Magnitude ◽  
Resolution Single ◽  
Single Molecule Localization Microscopy

<p>The light-promoted conversion of extensively used cyanine dyes to blue-shifted emissive products has been observed in various contexts. However, both the underlying mechanism and the species involved in this photoconversion reaction have remained elusive. Here we report that irradiation of heptamethine cyanines provides pentamethine cyanines, which, in turn, are photoconverted to trimethine cyanines. We detail an examination of the mechanism and substrate scope of this remarkable two-carbon phototruncation reaction. Supported by computational analysis, we propose that this reaction involves a singlet oxygen-initiated multi-step sequence involving a key hydroperoxycyclobutanol intermediate. Building on this mechanistic framework, we identify conditions to improve the yield of photoconversion by over an order of magnitude. We then demonstrate that cyanine phototruncation can be applied to super-resolution single-molecule localization microscopy, leading to improved spatial resolution with shorter imaging times. We anticipate these insights will help transform a common, but previously mechanistically ill-defined, chemical transformation into a valuable optical tool.</p>

Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction v1

protocols.io ◽  
2020 ◽  
Author(s):  
Joshua S. Titlow ◽  
Lu Yang ◽  
Richard M. Parton ◽  
Ana Palanca ◽  
Ilan Davis
Keyword(s):  
Neuromuscular Junction ◽  
Single Molecule ◽  
Super Resolution ◽  
Resolution Single
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