The role of Golgi bodies in polysaccharide sulphation in Fucuszygotes

1978 ◽  
Vol 32 (1) ◽  
pp. 337-356
Author(s):  
M.E. Callow ◽  
S.J. Coughlan ◽  
L.V. Evans
Keyword(s):  
Cell Wall ◽  
Alginic Acid ◽  
Soluble Fraction ◽  
Amorphous Layer ◽  
Acceptor Molecule ◽  
Golgi Bodies ◽  
Isolation And Characterization ◽  
Fucus Serratus

The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers—an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pole. Light- and electron-microscope autoradiographic pulse-chase experiments on 22-h zygotes using 35SO2-(4) show the Golgi bodies to be the sites of fucan sulphation. The isolation and characterization of isolated Golgi-rich fractions from 22-h zygotes shows that the first detectable labelled macromolecule is associated with these fractions 2 min after addition of 35SO2-(4). The sulphate acceptor molecule has been partially characterized. 35S-APS and 35S-paps are detectable in the soluble fraction 0.5 min after addition of 35SO2-(4). The results are discussed in relation to other published work on the differentiation of Fucus embryos and on polysaccharide sulphation.

Isolation and characterization of SRF accessory proteins

1993 ◽  
Vol 340 (1293) ◽  
pp. 325-332 ◽  
Keyword(s):  
Ternary Complex ◽  
Map Kinases ◽  
Serum Response Factor ◽  
Regulatory Element ◽  
Isolation And Characterization ◽  
Activation Of Transcription ◽  
Serum Response ◽  
Dna Binding Properties

Many genes which are regulated by growth factors contain a common regulatory element, the serum response element (SRE). Activation of transcription by the SRE involves a ternary complex formed between a ubiquitous factor, serum response factor (SRF), and a second protein, p62/TCF. We used a yeast genetic screen to isolate cDNAs encoding a protein, SAP-1, with the DNA binding properties of p62/TCF. The SAP-1 sequence contains three regions of homology to the previously uncharacterized Elk-1 protein, which also acts as an SRF accessory protein. Only two of these regions are required for cooperative interactions with SRF in the ternary complex. The third contains several conserved sites for the MAP kinases, whose activity is regulated in response to growth factor stimulation. We discuss the potential role of these proteins in regulation of the c-fos SRE.

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

Isolation and Characterization of Plant Cell Walls and Cell Wall Components

1988 ◽  
pp. 3-40 ◽  
Author(s):  
WILLIAM S. YORK ◽  
ALAN G. DARVILL ◽  
MICHAEL MCNEIL ◽  
THOMAS T. STEVENSON ◽  
PETER ALBERSHEIM
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Cell Wall Components ◽  
Isolation And Characterization ◽  
Wall Components

Isolation and characterization of plant cell walls and cell wall components

1986 ◽  
pp. 3-40 ◽  
Author(s):  
William S. York ◽  
Alan G. Darvill ◽  
Michael McNeil ◽  
Thomas T. Stevenson ◽  
Peter Albersheim
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Cell Wall Components ◽  
Isolation And Characterization ◽  
Wall Components

scholarly journals Isolation and Characterization of the PISTILLATA Ortholog Gene from Cymbidium faberi Rolfe

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 425 ◽  
Author(s):  
Yue Fei ◽  
Zhi-Xiong Liu
Keyword(s):  
Floral Development ◽  
Economic Value ◽  
Ectopic Expression ◽  
Anther Development ◽  
Protein Alignment ◽  
Isolation And Characterization ◽  
Petal Development ◽  
Potted Plant

Cymbidium faberi Rolfe is a very popular potted plant in China, Japan and Korea where it has been cultivated for centuries. The economic value of this popular native Asian orchid could be enhanced by changes in its floral traits. In Arabidopsis, PISTILLATA (PI) is involved in regulating petal and stamen development. In order to investigate the possible role of the PI ortholog involved in floral development, we isolated CyfaPI from C. faberi. Protein alignment and a phylogenetic tree grouped CyfaPI in the PI lineage. CyfaPI transcripts were detected in all floral organs, but were absent in leaves. Moreover, in flowers, the highest expression level of CyfaPI was present in the gynostemium and the lowest level was found in anther caps. In addition, ectopic expression of CyfaPI in Arabidopsis pi-1 mutant rescued petal development, and complement the development of filament-like structure (part of stamen), but failed to complement anther development in the stamen whorl. All these finding suggest that CyfaPI is mainly responsible for perianth and gynostemium development in C. faberi. Our data may help to trace the development of the gynostemium program and evolution in orchids.

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