Effects of vitamin A on growth and differentiation of human tracheobronchial epithelial cell cultures in serum-free medium

1989 ◽  
Vol 93 (1) ◽  
pp. 133-142
Author(s):  
D.P. Chopra ◽  
M.M. Klinger ◽  
J.K. Sullivan
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Vitamin A ◽  
Cell Cultures ◽  
Cell Multiplication ◽  
Serum Free Medium ◽  
Free Medium ◽  
Plasma Membrane Vesicles ◽  
Serum Free ◽  
Epithelial Cell Cultures

Differentiating epithelial cell cultures from human tracheobronchial epithelium have been propagated in serum-free medium. The major objective of this study was to examine the trophic effects of vitamin A on cell multiplication and morphology of the tracheal cell cultures. The cellular responses were analyzed in terms of growth kinetics, morphological and ultrastructural alterations and secretion of glycoconjugates. Cell cultures in control medium exhibited characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and numerous secretory vesicles in the cytoplasm. Vitamin A at 10(−6) M and 10(−7) M inhibited cell replication and enhanced the secretion of [3H]glucosamine-labeled glycoconjugates. Further, vitamin A increased the production of plasma membrane vesicles and acquisition by the cells of a highly secretory ultrastructure. This in vitro model of human epithelial cells will be important in the investigation of various aspects of growth and differentiation.

Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology

1991 ◽  
Vol 125 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Bertil G. Casslén ◽  
Michael J. K. Harper
Keyword(s):  
Epithelial Cells ◽  
Primary Cultures ◽  
Collagen Matrix ◽  
Human Endometrium ◽  
Serum Free Medium ◽  
Free Medium ◽  
Endometrial Stromal Cells ◽  
Prolonged Incubation ◽  
Serum Free ◽  
Endometrial Epithelial Cells

Abstract. The aim of the study was to explore the possibility of using human endometrial epithelial cells in serum-free culture as a sensitive assay for hormonal effects on the human endometrium. Glands were isolated following enzymatic digestion of the endometrial tissue and plated on a collagen matrix. The epithelial cells were grown in either medium containing serum or in supplemented serum-free medium. No morphologic difference was found between cells grown in these two media for up to 5 days, using either light or scanning electron microscopy. Secretion of prostaglandin F2α (PGF2α) in response to estradiol was not lower in serum-free medium than in medium containing serum for the first 2 days of culture, whereas secretion declined after prolonged incubation in the serum-free medium. This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid, the precursor for prostaglandin synthesis, to the medium. Co-culture of endometrial stromal cells did not influence the secretion of PGF2α by epithelial cells. We conclude that the secretion of PGF2α from primary cultures of human endometrial epithelial cells grown on collagen in serum-free medium can be used for a limited period as an assay of estrogenic effects on the human endometrium.

Characterization of growth and differentiation of normal human submandibular gland epithelial cells in a serum-free medium

1993 ◽  
Vol 54 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Masaya Okura ◽  
Kanemitsu Shirasuna ◽  
Tsutomu Hiranuma ◽  
Hideo Yoshioka ◽  
Hirokazu Nakahara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Submandibular Gland ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Normal Human

Growth of distal fetal rat lung epithelial cells in a defined serum-free medium

1991 ◽  
Vol 27 (8) ◽  
pp. 625-632 ◽  
Author(s):  
D. Jassal ◽  
R. N. N. Han ◽  
I. Caniggia ◽  
M. Post ◽  
A. K. Tanswell
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Rat Lung ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Fetal Rat Lung

Growth of mouse endometrial luminal epithelial cells in vitro: functional integrity of the oestrogen receptor system and failure of oestrogen to induce proliferation

1991 ◽  
Vol 128 (1) ◽  
pp. 115-120 ◽  
Author(s):  
F.-D. A. Uchima ◽  
M. Edery ◽  
T. Iguchi ◽  
H. A. Bern
Keyword(s):  
Epithelial Cells ◽  
Oestrogen Receptor ◽  
Continuous Exposure ◽  
Oestrogen Receptors ◽  
Serum Free Medium ◽  
Free Medium ◽  
Receptor System ◽  
Serum Free ◽  
Progestin Receptors ◽  
Luminal Epithelial Cells

ABSTRACT Normal endometrial luminal epithelial cells isolated from ovariectomized approximately 40-day-old BALB/cCrgl mice were purified by Percoll density gradient centrifugation and grown as primary cultures in collagen gel matrix and serum-free medium. Cells increased threefold in number during the 9-day culture period. Deletion of insulin, epidermal growth factor or bovine serum albumin resulted in decreased growth. Addition of any single factor to the unsupplemented medium had no effect. Relatively high levels of cytosolic oestrogen receptors and progestin receptors were demonstrable in the cultures. Addition of oestrogen did not enhance epithelial cell proliferation. On the contrary, all doses of oestrogen (180 fmol/l to 218 nmol/l) were inhibitory. Continuous exposure to oestradiol-17β (1·8 nmol/l) for 9 days in serum-free medium resulted in a decrease in cytosolic oestrogen receptors with an associated nuclear accumulation of oestrogen receptors. A corresponding increase in cytosolic progestin receptors was also observed, indicating that no qualitative modification of the oestrogen receptor system had occurred. Thus, as previously reported for vaginal epithelial cells, oestrogen, despite its stimulation of specific product synthesis (progestin receptors), did not increase proliferation of endometrial luminal epithelial cells in this culture system. Journal of Endocrinology (1991) 128, 115–120

Isolation and culture of colonic epithelial cells in serum-free medium

1985 ◽  
Vol 9 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Ariane A. Thompson ◽  
Sonya Dilworth ◽  
Robert J. Hay
Keyword(s):  
Epithelial Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Colonic Epithelial Cells ◽  
Serum Free
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