A new technique for measuring water transport across the seawater eel intestine

1986 ◽  
Vol 122 (1) ◽  
pp. 257-268 ◽  
Author(s):  
M. Ando ◽  
H. Sasaki ◽  
K. C. Huang
Keyword(s):  
Water Flux ◽  
Experimental System ◽  
New Technique ◽  
Perfusion Method ◽  
Marker Substances ◽  
Perfusate Flow ◽  
The Difference ◽  
A New Technique ◽  
Net Water Flux

A new technique was developed for measuring net water flux across the eel intestine in vitro. The new perfusion method was suitable for long duration experiments because of continuous oxygen supply to both the external and the perfusion medium. Net water flux was calculated directly from the difference between the rates of effluent and perfusate flow without measuring the concentration of marker substances. The calculated value of net flux appears to be reliable because: its direction is always from mucosa to serosa in both everted and non-everted intestine; ouabain diminishes it to zero; it is identical with the standard water flux obtained under zero perfusion; and it is identical with the value obtained by means of [14C]PEG following the previous perfusion method. The net water fluxes obtained by the new method were steadier than those obtained with the previous complicated perfusion method. In this new experimental system, the net water flux and the transepithelial potential difference (PD) decreased gradually with time, and were not restored by application of adrenergic agonists or cortisol. These parameters were stimulated by 5 mmol l-1 L-alanine, but not by D-glucose, L-valine or L-glycine, indicating a specific action of L-alanine.

A New Imaging Logging Technology Based on Magnetoacoustic Tomography with Magnetic Induction

2012 ◽  
Vol 433-440 ◽  
pp. 63-67 ◽  
Author(s):  
Hui Xia ◽  
Guo Qiang Liu ◽  
Wen Jing He
Keyword(s):  
High Resolution ◽  
Magnetic Induction ◽  
Experimental System ◽  
New Technique ◽  
Acoustic Tomography ◽  
Practical Application ◽  
Measurement Result ◽  
A New Technique ◽  
Copper Coil

Imaging logging is a new technique which has been developing in recent years, its’ silo facilities could collect large information with high resolution, and the measurement result is usually presented in graphics after processed. This paper presents a new imaging logging technique based on magnetic-acoustic Tomography with Magnetic Induction(MAT-MI), introduces its’ logging principle, gives detailed experimental system, makes a simple copper coil experiment and verifies the feasibility of the technique. These studies will lay a foundation for practical application of new imaging logging technology.

Applied pressures and net water flux across in vitro frog gastric mucosa.

1978 ◽  
Vol 235 (4) ◽  
pp. E361
Author(s):  
L Villegas
Keyword(s):  
Hydrostatic Pressure ◽  
Gastric Mucosa ◽  
Acid Secretion ◽  
Pressure Difference ◽  
Water Flux ◽  
Potential Difference ◽  
Electrical Potential Difference ◽  
Net Water Flux ◽  
Hydrostatic Pressure Difference

The effects of hydrostatic pressure differences up to 0.4 atm/413 cmH2O were studied on frog gastric mucosa in vitro. Net water flux, transmucosal electrical potential difference, and acid secretion were measured. A significant correlation between hydrostatistic pressure difference and net water flow (r=0.77) was obtained. The intercept of the regression line, at zero hydrostatic pressure difference, is 9.3 +/- 0.5 microliter/cm2.h, and the slope 42.9 +/- 3.2 microliter/cm2.atm.h. No significant correlation was obtained between the hydrostatic pressure difference and the transmucosal potential difference (P greater than 0.20), the acid secretion (P greater than 0.20), or the nonacidic chloride transport, measured as short-circuit current (P greater than 0.20). Hydrostatic water flux is compared to osmotically induced flux previously reported. It is proposed that the difference between hydrostatic and osmotic induced water fluxes is due to the area of cells exposed to the pressures. Only part of surface cells are directly exposed to the osmotic pressure due to the presence of restricted extracellular compartments.

New technique for detection of changes in QRS morphology of ECG signals

1983 ◽  
Vol 244 (4) ◽  
pp. H560-H566
Author(s):  
S. L. Blumlein ◽  
G. Harvey ◽  
V. K. Murthy ◽  
L. J. Haywood
Keyword(s):  
Low Frequency ◽  
New Technique ◽  
Frequency Noise ◽  
Third Order ◽  
Ecg Signals ◽  
Qrs Morphology ◽  
Ectopic Beats ◽  
The Difference ◽  
A New Technique ◽  
Electrocardiogram Ecg

With the use of the electrocardiogram (ECG) as a prototype signal, a new technique was devised for detecting signals embedded in noise. Averaged "normal" digitized ECG signals formed a template to which subsequent ECG QRS complexes were compared. The difference between the averaged template signals and subsequent normal beats was white noise, whereas the difference between the template and ectopic beats consisted of nonrandom signal variation. The template to new signal comparison for the zero-, first-, second-, and third-order differences utilized an approximate F test. Accurate detection of abnormal signals associated with high- and low-frequency noise is accomplished with this method, and the practical clinical utility of the method is under study.

A New Technique for the Three-Dimensional Study of the Spine In Vitro and In Vivo by Using a Motion-Analysis System

1997 ◽  
Vol 10 (4) ◽  
pp. 329???338 ◽  
Author(s):  
Xue-Cheng Liu ◽  
Guy Fabry ◽  
Luc Labey ◽  
Luc Van Den Berghe ◽  
Remi Van Audekercke ◽  
...  
Keyword(s):  
Motion Analysis ◽  
Three Dimensional ◽  
New Technique ◽  
Motion Analysis System ◽  
A New Technique ◽  
Analysis System
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