Amorphous phase MnOx/TiO2 catalysts were prepared by two different methods, their catalytic activities for low temperature selective catalytic reduction (SCR) of NOx with NH3 in the presence of excess oxygen were investigated. The catalysts were characterized by XRD, XPS and HRTEM. The results showed that the catalyst prepared by soft template method had better catalytic active than those prepared by sol-gel method, its catalytic property could reach 98.2% at 200°C. From the microstructure characterization, it could be known that the catalyst prepared by soft template had the shape of nanorod, this shape was contributed to the dispersion of the manganese oxides and possessed higher surface lattice oxygen concentration. Furthermore, narrow slit-shaped pores associated with rod-like particles could provide efficient transport pathways to reactant molecules and products. Due to these, the catalyst performed catalytic active very well.